An efficient in vitro plant regeneration system for the medicinal plant Teucrium stocksianum Boiss.

Abstract

An efficient and rapid plant regeneration system via direct organogenesis was established for Teucrium stocksianum Boiss. (Lamiaceae), an endangered and valuable medicinal plant. Hypocotyl explants excised from seedlings germinated in vitro were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of kinetin and indoleacetic acid (IAA) to induce shoot formation. Differentiation of multiple shoots was initiated within 3 weeks of culture. Optimal regeneration was achieved on medium containing 3 mg/l kinetin and 0.5 mg/l IAA. This particular medium composition significantly improved the production of multiple shoots directly from hypocotyl explants compared to other combinations of plant growth regulators. Root induction was achieved on half-strength MS medium containing indole-3-butyric acid. Rooted plantlets were successfully acclimatized, with a survival rate of 75-80%. The protocol developed in this study could be used for long-term in vitro conservation and mass propagation of this species.

In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

“…Similar to our observation, Nathar and Yatoo (2014) found a lower salt concentration to be effective for in vitro root induction. High efficiency of IBA for in vitro root induction compared to other auxins has been earlier reported (Bouhouche and Ksiksi 2007). However, root induction with a medium containing a higher concentration (1.0 mg L -1 ) of IBA was accompanied by a profuse development of calli, which inhibited further development of roots.…”

Evaluation on the effects of P. ostreatus spent mushroom compost and BAP hormone towards C. nutans in vitro culture

“…Tissue culture is one of the techniques, which is powerful for conservation and rapid multiplication of plant species [6]. It is a useful propagation and conservation in faster rate for valuable plant that is really required by pharmaceutical line for medicine production especially from plant herbs extract to treat diseases.…”

“…As for example, pH is maintained at 5.8 throughout the plant tissue culture before being autoclaved [7]. Then, for growing temperature in the growth chamber is at 25°C [6].…”

In vitro propagation and conservation of Satureja avromanica Maroofi—an indigenous threatened medicinal plant of Iran

“…Results also revealed a slight inhibitory effect of high IBA level on rooting, which has been proposed to be a general phenomenon (Swarna and Ravindhran 2012). Optimal rhizogenesis using IBA has been shown for other plant species, including Viola pilosa (Soni and Kaur 2014), Talinum triangulare (Swarna and Ravindhran 2012) and Teucrium stocksianum (Bouhouche and Ksiksi 2007).…”

“…The rooted plantlets were successfully acclimatized and eventually established in plastic pots containing peat moss, coco peat and perlite in the ratio of 1:1:1 under greenhouse condition. Of 100 plants transferred to greenhouse, 60 plants survived showing a survival rate of 60 % which was lower than Isodon wightii with 70 % (Thirugnanasampandan et al 2010), Teucrium stocksianum with 75-80 % (Bouhouche and Ksiksi 2007), and Justicia gendarussa with 90 % of survival rate (Dennis Thomas and Yoichiro 2010).…”