In vitro propagation and conservation of Satureja avromanica Maroofi—an indigenous threatened medicinal plant of Iran

Abstract

An efficient and rapid in vitro propagation system for Satureja avromanica, a rare and endangered folk medicinal plant of Iran was developed through the shoot tip and leaf disc explants. Nodal and leaf explants from wild plants were established on MS and WPM media supplemented with BA, BAP and TDZ (0, 0.1, 0.5, 1, 1.5, 2, 5 and 10 mgl ), respectively. Based on results, the highest mean shoot number (6.21) was obtained on MS medium supplemented with 2 mgl −1 BA. Regarding the shoot elongation, MS supplemented with 2 mgl −1 TDZ and MS containing 5 mgl −1 BA showed the longest shoots (4.82 and 4.39 cm, respectively) after 6 weeks of culture. As a matter of fact, increasing all three tested cytokinins levels led to enhancement of explant response frequency and regenerated shoot number. On the other side, WPM medium supplemented with 0.1 mgl −1 IBA was found suitable for rooting of regenerated shoots. RAPD molecular analysis revealed genetic stability of in vitro raised plants. In conclusion, individual application of BA, BAP and TDZ were in favor of S. avromanica direct shoot regeneration while treatment media with a combination of IBA and BA as well as 2,4-D and TDZ resulted in callogenesis in most explants. Finally, the in vitro raised plantlets were acclimatized and successfully established in the greenhouse conditions. Our developed protocol can be employed for the large-scale micropropagation and conservation of S. avromanica as a threatened medicinal plant.

Establishment of an Improved, Efficient and Eco-Friendly Micropropagation System in Salacia Chinensis L. An Endangered Anti-Diabetic Medicinal Plant

“…BAP is structurally stable and the plant cells ability to its easy assimilation makes this kind of cytokinin stand out among the others (Ahmad et al, 2013). BAP strong promotive effects on shoot regeneration were reported previously (Rao and Purohit, 2006;Mozafari et al, 2015). Suitable individual concentration of BAP was 1.5 mg/l for shoot induction in node, while this rate was 2.0 mg/l for Kin.…”

Micropropagation of arrow cane, Gynerium sagittatum (Aubl.) P. Beauv. cv. Criolla, Criolla 1, and Martinera, in a double-phase medium

“…A second limiting factor for in vitro grown plants transferred to ex vitro is rapid and severe tissue dehydration (Kumari et al, 2017;Mozafari et al, 2015). In vitro plants are maintained in a watersaturated closed atmosphere and established in a cultured medium with > 90 % water.…”

Genetic stability of micropropagated plants of Deschampsia antarctica Desv. during long-term in vitro culture

“…При дослідженні клонованих in vitro рослин цінного лікарського виду Viola pilosa за допомогою RAPD-i ISSR-маркерів сомаклональної мінливості у них не виявлено [27]. Не виявлено також мінливості за даними RAPD-аналізу і серед мікроклонів лікарської рослини Satureja avromanica, яка знаходиться під загрозою зникнення в Ірані, що дало авторам підставу рекомендувати розроблені протоколи мікроклонування для масштабного розмноження і збереження виду [22].…”