An easy-to-use wound dressing gelatin-bioactive nanoparticle gel and its preliminary in vivo study

Abstract

Beyond promoting hard tissue repairing, bioactive glasses (BGs) have also been proved to be beneficial for wound healing. Nano-scale BGs prepared by sol-gel method were found to have a better performance as they have a larger specific surface area. In this work, bioactive nanoparticles (nBPs) with mean diameter of 12 nm (BP-12) instead of conventional BGs were mixed with gelatin to form an easy-to-use hydrogel as a dressing for skin wound. It was found that the composite of BP-12 and gelatin could form a hydrogel (BP-12/Gel) under 25 °C, which showed pronounced thixotropy at a practically accessible shear rate, therefore become easy to be used for wound cover. In vitro, the composite hydrogel of BP-12 and gelatin had good biocompatibility with the fibroblast cells. In vivo, rapid cutaneous-tissue regeneration and tissue-structure formation within 7 days was observed in the wound-healing experiment performed in rats. This hydrogel is thus a promising easy-to-use wound dressing material.

Use of Nanoparticles in Tissue Engineering and Regenerative Medicine

“…nBGs of either unimodal or bimodal (narrow) pore size distribution had higher bioactivity compared to the nBGs with smooth surface morphology. Wang and colleagues studied nBGs with a diameter of 12 nm (BP-12) instead of mixing conventional bioglass (diameter of 200 nm) with gelatin to manufacture a simple hydrogel for wound dressing application (Wang C. et al, 2016). Composition of gelatin with BP-12 could provide hydrogel with pronounced thixotropy (becoming less viscous) characteristic at a practically usable shear rate.…”

Inorganic Nanomaterials in Tissue Engineering

“…Wang and colleagues studied bioactive glasses nanoparticles mixed with gelatin for the production of hydrogels intended for wound dressing. The mixture was able to promote faster tissue regeneration and a more effective wound healing, due to the synergic effect of the bioactive glass and gelatin, within seven days after implantation in a murine model [77]. Samadian et al developed an electrospun cellulose scaffold loaded with hydroxyapatite (HP) by means of electrospinning technique.…”

Design, preparation, and characterization of silk fibroin/carboxymethyl cellulose wound dressing for skin tissue regeneration applications

“…Gelatin has also been a fitted polymer in wound healing due to its mechanical and physicochemical characteristics, and along with its sufficient qualifications, gelatin adopts itself with the skin thermally, which helps to remain in its physical shape. [ 28 ] Moreover, gelatin addition along CMC could increase the hemostatic activity and cell proliferation, and migration of the bottom layer. [ 29,30 ]…”