An atypical Staphylococcus aureus intramammary infection in a dairy herd

Laevens, Hans; Devriese, L.; Deluyker, Hubert; Hommez, J.; Kruif, Aart de

doi:10.1016/s0378-1135(96)00077-6

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…Coagulase-negative variants of Staph. aureus strains were first isolated from milk samples derived from subclinical mastitis cases in dairy cattle (Fox et al 1996; Laevens et al 1996; Matthews et al 1997; Malinowski et al 2009). In some other clinical studies, detection of coagulase-negative Staph.…”

Section: Discussionmentioning

confidence: 99%

“…The known specificity of all these tests has previously been used to identify atypical variants of Staph. aureus strains isolated from clinical specimens and mastitis milk samples (Kloos & Schleifer, 1975; Heltberg & Bruun, 1984; Smyth et al 1988; Bodén et al 1989; Fox et al 1993; Mackay et al 1993; Laevens et al 1996; Matthews et al 1997; Personne et al 1997; Wilkerson et al 1997; Mlynarczyk et al 1998; Smole et al 1998; Al Obaid et al 1999; van Griethuysen et al 2001; Garbacz et al 2002; Malinowski et al 2009). In fact, Staph.…”

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confidence: 99%

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A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk

Akineden

Hassan

Schneider

et al. 2010

Journal of Dairy Research

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Bacteriological analysis of milk samples from quarters of a dairy cow suffering from subclinical mastitis yielded two isolates of Staphylococcus aureus which gave a negative reaction in the standard coagulase test. Both isolates were also clumping factor and thermonuclease negative, and gave a negative reaction in the Staphaurex® test. The isolates were identified by using commercial biochemical systems, and by PCR analysis of different staphylococcal cell surface protein and exoprotein genes. Further molecular identification of the isolates, which included sequencing of the 16S rRNA gene and RT-PCR of coagulase (coa), clumping-factor (clfA) and thermonuclease (nuc) genes, was consistent with the diagnosis phenotypically 'coagulase-negative variant of Staph. aureus'. The fact that coagulase-negative Staph. aureus variants can occur in the context of intramammary infections in cattle may result in the incorrect diagnosis 'coagulase-negative staphylococci (CNS)' in routine mastitis diagnostic, at least in rare cases. To fully ensure correct species diagnosis, sequencing of the 16S rRNA gene and amplification of specific genes such as coa is necessary in these cases.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk

Akineden

Hassan

Schneider

et al. 2010

Journal of Dairy Research

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“…(1992) reported the lack of clumping factor activity in S. aureus mastitis strains of more than 50% of the investigated field strains. Laevens et al. (1996) isolated an atypical clumping factor negative S. aureus strain as a cause of intramammary infection in a dairy herd.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of six commercial identification kits for the identification of Staphylococcus aureus isolated from bovine mastitis

2005

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Aims: Comparison of six commercially available in human medicine well‐established slide agglutination systems for the identification of Staphylococcus aureus. Methods and Results: Slide agglutination tests were compared with the conventional tube coagulase test, biochemical identification and with the molecular identification by polymerase chain reaction (PCR) amplification of species‐specific parts of the gene encoding the 23S RNA. Systems evaluated included Masta‐Staph® (Mast Diagnostics), Staphylase‐Test® (Oxoid), Staphytect‐Plus® (Oxoid), Staphyloslide Latex® (Becton Dickinson), Slidex Staph Plus® (bioMérieux) and Dry Spot Staphytect Plus® (Oxoid). A total of 141 staphylococcal strains isolated from cases of bovine mastitis including 90 S. aureus, 14 Staphylococcus epidermidis, 10 Staphylococcus warneri, 13 Staphylococcus xylosus, 11 Staphylococcus haemolyticus and three other coagulase‐negative staphylococci were tested with each method. Staphylococcus aureus strains were selected by macrorestriction analysis with pulsed field gel electrophoresis (PFGE). Only genetically unrelated strains were included in the study. The sensitivities and specificities of the test were as follows: Masta‐Staph® 86·7 and 90·1%, Staphylase‐Test® 78·4 and 85·1%, Staphytect‐Plus® 81·1 and 86·5%, Staphyloslide Latex® 77·8 and 84·4%, Slidex Staph Plus® 77·8 and 84·4%, Dry Spot Staphytect Plus® 75·6 and 83·0%. Conclusions: The results of this evaluation suggest that the six slide agglutination methods tested can provide rapid identification of S. aureus also from bovine mastitis. The sensitivity and specificity seems to be less than those reported from human S. aureus isolates. Significance and Impact of the Study: This is one of the first comparative reported investigations about the applicability of different commercially available slide agglutination tests for the detection of S. aureus from bovine mastitis using PFGE selected clinical isolates.

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“…Although there are different typing sets, S. aureus from bovine mastitis has mainly been characterized by using the human international set of phages according to the technique of Blair and Williams (1961), (Wallace et al, 1962;Frost, 1967;Laevens et al, 1996). Other phenotypic characterization includes haemolytic activity, biochemical and enzymatic reactions, including activity on selective media, and antibiotic susceptibility patterns (Frost, 1967;Matsunaga et al, 1993;Aarestrup et al, 1995a;Laevens et al, 1996). These works show marked differences among the characteristics of S. aureus isolates, with the different methods for identification and typing used, without establishing the relationship between bacterial type and the clinicopathological reaction of the mammary gland.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Characteristics of Staphylococcus aureus Isolated from Bovine Mastitis in Israeli Dairy Herds

Younis¹,

Leitner²,

Heller

et al. 2000

Journal of Veterinary Medicine, Series B

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A study of the characterization of the phenotypic patterns of Staphylococcus aureus strains isolated from bovine subclinical mastitis in Israeli dairy herds and their correlation with the severity of the disease was undertaken. A total of 400 chronically S. aureus-infected Israeli-Holstein cows, from 15 dairy herds were included in this study. Based on the results of the biochemical reactions, of the anti-biogram and phage typing, one major type of S. aureus was determined in each herd, its prevalence being between 54 and 100% of the total isolates from that same herd. The majority of the isolates were found to be non-haemolytic (62.7%). The most common phage type was 3/A,3/C,55,71, which was predominant in five herds. In two herds none of the isolates (24) were typable by this set of phages. All isolates were susceptible to methicillin, erythromycin, cephalotin, norfloxacin, trimethoprin-sulphamethoxazole and novobiocin. Most isolates were resistant to penicillin (96.6%) and 52% to oxytetracyclin. Differences in protein patterns between 50 and 36 kDa were found by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis. No correlation between any combination of the phenotypic characteristics was found when correlation was done with milk yield and somatic cell count, corresponding to the 6 months before sampling. Otherwise, a positive correlation was found between type of haemolysis and the N-acetyl-beta-glucosaminidase (NAGase) values. In milk from quarters infected with the-non-haemolytic strains, the level of NAGase was significantly lower (P < 0.05) than that from quarters infected with the haemolytic strains (69.7 and 105.9, respectively). However, the level of NAGase activity in the milk of the quarters infected with the non-haemolytic strains was significantly higher (P < 0.05) when compared to the milk of quarters infected with coagulase-negative staphylococci (43.5).

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An atypical Staphylococcus aureus intramammary infection in a dairy herd

Cited by 3 publications

References 7 publications

A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk

A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk

Evaluation of six commercial identification kits for the identification of Staphylococcus aureus isolated from bovine mastitis

Phenotypic Characteristics of Staphylococcus aureus Isolated from Bovine Mastitis in Israeli Dairy Herds

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