Evaluation of six commercial identification kits for the identification of Staphylococcus aureus isolated from bovine mastitis

Zschöck, M.; Nesseler, A.; Sudarwanto, I.

doi:10.1111/j.1365-2672.2004.02470.x

Cited by 16 publications

(8 citation statements)

References 22 publications

(20 reference statements)

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“…Only 5 strains isolated from cow mastitis failed in the test. Similar to our results are the findings of Zschöck et al (29) who evaluated 6 commercial agglutination kits for S. aureus detection in bovine mastitis. The authors conclude that slide agglutination methods can provide rapid identification of S. aureus from bovine mastitis as well, however the sensitivity and specificity of these tests is lower than those reported for human isolates.…”

Section: Discussionsupporting

confidence: 92%

Detection of Staphylococcus Aureus Among Coagulase Positive Staphylococci from Animal Origin Based on Conventional and Molecular Methods

Rusenova

Rusenov

2017

Macedonian Veterinary Review

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The present study aimed to detect Staphylococcus aureus (S. aureus) among other coagulase positive staphylococci from animal origin by using conventional methods (biochemical tests and latex agglutination) and a molecular method, based on the nuc gene, as the gold standard and to assess the usefulness of these methods. For this purpose, total of 344 staphylococcal isolates were collected and analysed. A total of 156 isolates suspicious for S. aureus were detected by a conventional biochemical method -88 from cows, 18 from goats, 7 from pigs, 17 from poultry, 7 from rabbits and 19 from dogs. The majority of S. aureus strains gave typical biochemical reactions with the exception of 30 (19.2%) and 25 (16%) that were VP negative and weak positive in fermenting mannitol, respectively. Twelve strains were found to be non-haemolytic (7.7%) and four strains did not ferment trehalose (2.6%). Other staphylococci were identified as S. pseudintermedius (n = 103), S. hyicus (n = 23) and the rest were coagulase-negative staphylococci. Latex agglutination test resulted in rapid positive reactions with S. aureus with exception of 5 strains (3.2%) from cow mastitis milk. Positive agglutination reactions were also established with S. pseudintermedius, and S. hyicus. PCR confirmed all strains that were preliminary identified as S. aureus by amplification of 270 bp fragment of nuc gene specific for this species. The atypical reactions in certain strains established in this study have shown that the precise detection of S. aureus from animal origin should be done by combination of conventional and molecular methods.

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Section: Discussionsupporting

confidence: 92%

Detection of Staphylococcus Aureus Among Coagulase Positive Staphylococci from Animal Origin Based on Conventional and Molecular Methods

Rusenova

Rusenov

2017

Macedonian Veterinary Review

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“…A latex agglutination test that detects PBP2a, the penicillin-binding protein that mediates methicillin resistance, and nucleic acid amplification methods to detect mecA , the gene encoding PBP2a, are also available. 105–107 A detailed discussion of the advantages and disadvantages of these various methods is beyond the scope of this article, except to note that sensitivity and specificity of any single test is about 95%, no test is perfect, and most clinical laboratories perform confirmatory testing. Presently, the only way to determine susceptibility to non-beta-lactam antibiotics is by standard susceptibility testing.…”

Section: Diagnosismentioning

confidence: 99%

Community-associated meticillin-resistant Staphylococcus aureus

et al. 2010

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Summary Methicillin-resistant Staphylococcus aureus (MRSA) is endemic in hospitals worldwide and a significant cause of morbidity and mortality. Healthcare-associated MRSA infections occur in individuals with predisposing risk factors for disease, such as surgery or presence of an indwelling medical device. By contrast, community-associated MRSA (CA-MRSA) infections often occur in otherwise healthy individuals who lack such risk factors. In addition, CA-MRSA infections are epidemic in some countries. These observations suggest that CA-MRSA strains are more virulent and transmissible than traditional hospital-associated MRSA strains. Relatively limited treatment options for CA-MRSA infections compound the problem of enhanced virulence and transmission. Although progress has been made toward understanding emergence of CA-MRSA, virulence, and treatment of infections, our knowledge in these areas remains incomplete. Here were review the most current knowledge in these areas and provide perspective on future outlook for prophylaxis and/or new therapies for CA-MRSA infections.

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“…As such, the specificity and sensitivity of this diagnostic test depend on the presence and proper function of the above-mentioned proteins on the surface of S. aureus. Some studies have reported low sensitivities and specificities for bovine S. aureus detection with latex agglutination-based tests, but the possible reasons for such observations have not yet been examined (35,45).…”

mentioning

confidence: 99%

SpA, ClfA, and FnbA Genetic Variations Lead to Staphaurex Test-Negative Phenotypes in Bovine Mastitis Staphylococcus aureus Isolates

2011

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Staphylococcus aureus encodes many proteins that act as virulence factors, leading to a variety of diseases, including mastitis in cows. Among these virulence factors, SpA, ClfA, ClfB, FnbA, and FnbB are important for the ability of S. aureus to adhere to and invade host cells as well as to evade host immune responses. The interaction between these S. aureus surface proteins and human immunoglobulin G and fibrinogen that are coupled to latex particles is utilized to induce latex agglutination reactions, which are used widely in diagnostic kits for confirmation of presumptive S. aureus isolates. In this study, the Staphaurex latex agglutination test was performed on a collection of confirmed bovine mastitis S. aureus isolates. Notably, 54% (43/79 isolates) of these isolates exhibited latex agglutination-negative phenotypes (Staphaurex-negative result). To gain insights into the reasons for the high frequency of Staphaurex-negative bovine mastitis S. aureus isolates, the spa, clfA, clfB, fnbA, and fnbB genes were examined. Specific genetic changes in spa, clfA, and fnbA, as well as a loss of fnbB, which may impair SpA, ClfA, FnbA, and FnbB functions in latex agglutination reactions, were detected in Staphaurex-negative S. aureus isolates. The genetic changes included a premature stop codon in the spa gene, leading to a truncated SpA protein that is unable to participate in S. aureus cell-mediated agglutination of latex particles. In addition, clfA and fnbA genetic polymorphisms were detected that were linked to ClfA and FnbA amino acid changes that may significantly reduce fibrinogen-binding activity. The genetic variations in these S. aureus isolates might also have implications for their bovine mastitis virulence capacity.

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Evaluation of six commercial identification kits for the identification of Staphylococcus aureus isolated from bovine mastitis

Cited by 16 publications

References 22 publications

Detection of Staphylococcus Aureus Among Coagulase Positive Staphylococci from Animal Origin Based on Conventional and Molecular Methods

Detection of Staphylococcus Aureus Among Coagulase Positive Staphylococci from Animal Origin Based on Conventional and Molecular Methods

Community-associated meticillin-resistant Staphylococcus aureus

SpA, ClfA, and FnbA Genetic Variations Lead to Staphaurex Test-Negative Phenotypes in Bovine Mastitis Staphylococcus aureus Isolates

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