A coagulase-negative variant of<i>Staphylococcus aureus</i>from bovine mastitis milk

Akineden, Ömer; Hassan, Abdulwahed Ahmed; Schneider, Elisabeth; Usleber, Ewald

doi:10.1017/s0022029910000774

Cited by 23 publications

(22 citation statements)

References 38 publications

(48 reference statements)

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“…Six S. aureus isolates did not clot rabbit plasma, but showed positive results in Voges-Proskauer and latex agglutination tests and amplified femA and coa genes. S. aureus strains isolated from bovine mastitis that failed to coagulate rabbit plasma have been previously described (Vieira-Da-Mota et al, 2001;Capurro et al, 2009;Akineden et al, 2011). Vieira-Da-Mota et al (2001) reported S. aureus strains isolated from bovine mastitis that were negative in the tube coagulase test, but showed a positive result in the PCR amplification of the coa gene.…”

Section: Discussionmentioning

confidence: 96%

Species-level identification of staphylococci isolated from bovine mastitis in Brazil using partial 16S rRNA sequencing

Lange

Brito

Reis

et al. 2015

Veterinary Microbiology

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Section: Discussionmentioning

confidence: 96%

Species-level identification of staphylococci isolated from bovine mastitis in Brazil using partial 16S rRNA sequencing

Lange

Brito

Reis

et al. 2015

Veterinary Microbiology

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“…However, they are called S. aureus-like strains because of the need to reaffirm their belonging to the species S. aureus. For this purpose, further molecular identification of the isolates, which included sequencing of 16S rRNA gene and RT-PCR of coagulase (coa) is needed (1). In recent years, several authors reported a significant role of atypical (freecoagulase negative) S. aureus infections in animals and humans.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Staphylococcus aureus and Staphylococcus aureus – like strains isolated from table eggs

Pyzik

Marek

Hauschild

2014

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to provide a detailed phenotypic and genotypic characterisation of Staphylococcus aureus strains and the group of microorganisms with unusual biochemical patterns (called Staphylococcus aureus-like) isolated from table chicken eggs. All of the strains tested exhibited resistance to at least one of the 17 antibiotics tested, and 55.55% of isolates were found to be resistant to five or more of them. PCR used for detection of the methicillin resistance gene (mecA) confirmed the presence of a specific product of 533 bp in the case of two of the isolated S. aureus-like strains. Analysis of the phylogenetic relationship between eight of S. aureus and ten S. aureus-like strains distinguished 18 macrorestriction profiles following digestion with SmaI endonuclease, indicating that there were no identical strains with the same macrorestriction profile. However, the presence of methicillin-resistant strains indicates a serious risk to consumer health.

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“…Staphylocoagulase production represents the major criterion for the detection of S. aureus isolates. As coagulase-deficient clinical isolates of S. aureus have been described, additional use of chromogenic media in S. aureus detection was postulated to represent a highly specific (97%) and sensitive (99% after 48 h) tool in the identification of the organism (1,3,4,7,8,10). In this study, we further investigated the genotypic and phenotypic characteristics of a coagulase-and ␣-glucosidase-deficient variant of S. aureus obtained from bovine mastitis milk.…”

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confidence: 99%

A Coagulase- and α-Glucosidase-Negative Variant of Staphylococcus aureus: a Challenge for Routine Microbiological Diagnostics

Johler¹,

Moser²,

Engl³

et al. 2012

J Clin Microbiol

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Staphylococcus aureus can cause serious infections, toxinoses, and life-threatening illnesses. Staphylocoagulase production represents the major criterion for the detection of S. aureus isolates. As coagulase-deficient clinical isolates of S. aureus have been described, additional use of chromogenic media in S. aureus detection was postulated to represent a highly specific (97%) and sensitive (99% after 48 h) tool in the identification of the organism (1,3,4,7,8,10). In this study, we further investigated the genotypic and phenotypic characteristics of a coagulase-and ␣-glucosidase-deficient variant of S. aureus obtained from bovine mastitis milk.Strain MSSA_129 (methicillin-susceptible S. aureus) was identified as S. aureus by species-specific PCR, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) (99.90% identification), and DNA microarray and could be assigned to spa type t543, clonal complex CC479, agr type II, and capsule type 8. It exhibited alpha, beta, and delta hemolysis and was slightly yellow pigmented. Staphaureux (Thermo Fisher Scientific, Wohlen, Switzerland), DNase, and egg yolk test yielded positive results. Still, no opaque zone was visible on rabbit plasma fibrinogen (RPF) agar (Oxoid, Cambridge, United Kingdom) ( Fig. 1). On S. aureus ID agar (SAID; bioMérieux, La Balme les Grottes, France), bright yellow colonies instead of the characteristic green colonies were formed (Fig. 2). DNA microarray detected neither antibiotic resistance determinants nor genes involved in toxic shock and staphylococcal scalded-skin syndrome. While the classical enterotoxin genes (sea, seb, sec, sed, and see) were not detected, the strain exhibited the egc cluster carrying genes encoding newly described staphylococcal enterotoxins and staphylococcal enterotoxin-like superantigens.Determination of the nucleotide sequence of coa revealed a deletion at nucleotide position 653 within the D2 region of the gene, leading to a frameshift. Although coa is known to represent a highly polymorphic region, it can be divided into six common regions: the signal sequence, the D1 and D2 regions enabling contact with prothrombin, the central region, a repeat region, and the C-terminal sequence (5, 9, 11, 12). The frameshift detected results in the reading of a premature stop codon at amino acid position 224, thus rendering the polypeptide abnormally short and most likely not functional.The ␣-glucosidase gene of MSSA_129 and its upstream region were compared to sequences available at GenBank. Although no strain possesses an ␣-glucosidase gene exactly identical over its full length to the one found in MSSA_129, no unique amino acid changes were found. However, variability of amino acid sequences on 12 positions (position 43, 83, 88, 133, 439, 448, 454, 462, 492, 514, 519, and 539) within the available sequences was noticed. Considering the amino acids found at these positions, strain MSSA_129 showed a unique combination. Directly upstream of the ␣-glucosidase gene (malA), we identified a sequence...

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A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk

Cited by 23 publications

References 38 publications

Species-level identification of staphylococci isolated from bovine mastitis in Brazil using partial 16S rRNA sequencing

Species-level identification of staphylococci isolated from bovine mastitis in Brazil using partial 16S rRNA sequencing

Characterisation of Staphylococcus aureus and Staphylococcus aureus – like strains isolated from table eggs

A Coagulase- and α-Glucosidase-Negative Variant of Staphylococcus aureus: a Challenge for Routine Microbiological Diagnostics

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