An assay for quantification of white spot syndrome virus using a capture ELISA

Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus

“…With our method, WSSV present at 2.5 ng/mL can be routinely detected. Such a detection level is 20 times lower than that measurably by ELISA (Chen et al, 2002). We tested SPR measurement of the 50-time diluted hemolymph from infected shrimps without any other pretreatments at A1-modified sensor surface.…”

“…In recent years, the pathological effects of and preventive measures for this virus have been extensively studied in an attempt to control possible massive disease outbreaks. Many diagnostic tools, such as gene probes (Duarand et al, 1996), polymerase chain reaction (PCR) (Lo et al, 1996), flow cytometry (Sahul Hameed et al, 2006) and immunological methods (Chen et al, 2002), have been developed to control the spread of WSSV from parent shrimps and imported shrimps. However, these analyses are usually expensive, lengthy and labor-consuming.…”

Detection and titration of white spot syndrome virus using a Blue‐Cell ELISA

“…White spot syndrome virus (WSSV) is the causative agent of a shrimp disease which affects most of the commercially cultivated marine shrimp species globally and has become the leading cause of production losses (Flegel 1997). To monitor the progression of this disease in infected animals, the number of viral genomes has been determined by a competitive polymerase chain reaction (PCR) (Tang & Lightner 2000), and the concentration of viral proteins has been measured using a capture enzyme‐linked immunosorbent assay (ELISA) (Chen, Wang & Shih 2002). However, study and quantification of WSSV is still hampered by the lack of a continuous cell culture system for shrimp.…”

“…These include PCR (Wang et al . 1997), two‐step PCR (Lo, Leu, Ho, Chen, Peng, Chen, Chou, Yeh, Huang, Chou, Wang & Kou 1996), ISH (Wang, Tsai & Chen 1998), Western blot (Nadala, Tapay, Cao & Loh 1997), dot‐blot nitrocellulose enzyme immunoassay (DB‐NC‐EIA) (Nadala & Loh 2002), competitive ELISA (Hameed, Anilkumar, Raj & Jayaraman 1998), and capture ELISA (Chen et al . 2002).…”

Development of a multiplex PCR system for the simultaneous detection of white spot syndrome virus and hepatopancreatic parvovirus infection

“…Several molecular diagnostic methods, such as polymerase chain reaction (PCR) (Lo, Leu, Ho, Chen, Peng, Chen, Chou, Yeh, Huang & Chou ; Manjanaik, Umesha & Karunasagar ; Pantoja & Lightner ; Sukhumsirichart, Wongteerasupaya, Boonsaeng, Panyim, Sriurairatana, Withyachumnarnkul & Flegel ), PCR‐enzyme‐linked immunosorbent assay (ELISA) (Sukhumsirichart, Kiatpathomchai, Wongteerasupaya, Withyachumnarnkul, Flegel, Boonseang & Panyim ), ELISA (Chen, Wang & Shih ), loop‐mediated isothermal amplification (LAMP) (Kono, Savan, Sakai & Itami ; Nimitphak, Kiatpathomchai & Flegel ), real‐time PCR (Sritunyalucksana, Srisala, McColl, Nielsen & Flegel ; Tan, Soon, Lee, Shariff, Hassan & Omar ; Yuan, Zhang, Chang, Jia, Hemmingsen & Dai ), LAMP combined with lateral flow chromatographic strips (LFCS), in situ hybridization (Pantoja & Lightner ; Phromjai, Boonsaeng, Withyachumnarnkul & Flegel ) and specific immune chromatographic tests (ICTs) (Wang & Zhan ), have been developed for the detection of HPV and WSSV.…”