Development of a multiplex PCR system for the simultaneous detection of white spot syndrome virus and hepatopancreatic parvovirus infection

Jeeva, Subbiah; Kim, Namil; Jang, In‐Kwon; Choi, Tae-Jin

doi:10.1111/are.12045

Cited by 11 publications

(6 citation statements)

References 57 publications

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“…), Multiplex PCR (Jeeva et al . ) and qPCR(Mendoza‐Cano and Sánchez‐Paz ), in situ hybridization method(Chang et al . ) and recently developed loop‐mediated isothermal method (Chang et al .…”

Section: Introductionmentioning

confidence: 99%

Prevalence and distribution of W hite S pot S yndrome V irus in cultured shrimp

Hossain

Nandi

Siddique

et al. 2014

Lett Appl Microbiol

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White Spot Syndrome Virus (WSSV) is a dsDNA virus causing White Spot Syndrome Disease (WSSD) in shrimp with almost 100% morality rate within 3-10 days. In Bangladesh, WSSD is one of the major impediments of shrimp farming. This study first investigated the prevalence and distribution of WSSV in cultured shrimps of the coastal regions in Bangladesh. A total of 60 shrimp samples, collected from the 25 shrimp farms of different coastal regions (Satkhira, Khulna, Bagerhat and Cox's Bazar), were analysed during 2013-2014 by conventional PCR using VP28 and VP664 gene-specific primers; 39 of 60 samples were found WSSV positive. SYBR green real-time PCR using 71-bp amplicon for VP664 gene correlated well with conventional PCR data. The prevalence rates of WSSV among the collected 60 samples were Satkhira 79%, Khulna 50%, Bagerhat 38% and Cox's Bazar 25%. Sequencing of WSSV-positive PCR amplicons of VP28 showed 99% similarity with WSSV NCBI Ref/Seq Sequences. Molecular analysis of the VP28 gene sequences of WSSV revealed that Bangladeshi strains phylogenetically affiliated to the strains belong to India. This work concluded that WSSV infections are widely distributed in the coastal regions cultured shrimp in Bangladesh.

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Section: Introductionmentioning

confidence: 99%

Prevalence and distribution of W hite S pot S yndrome V irus in cultured shrimp

Hossain

Nandi

Siddique

et al. 2014

Lett Appl Microbiol

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“…In Korea, co‐infection with a combination of WSSV and Vibrio anguillarum or DHPV and EHP has been reported in cultured P. vannamei (Jang et al, 2014; Lee et al, 2023). Co‐infection with WSSV and DHPV was reported in 2008 and 2014 in Korea, with WSSV being the primary pathogen (40–100% prevalence) while DHPV had a lower prevalence (3.5–23.5%) (Jang et al, 2008; Jeeva et al, 2014). However, in this study, the detection rate of DHPV (100%) was higher than that of WSSV (49.4%) in P. vannamei .…”

Section: Discussionmentioning

confidence: 99%

“…For DHPV detection, PCR was performed using primers 1414F/1750R, yielding a 338-bp product (Jeeva et al, 2014). Each PCR mixture was prepared in a final volume of 25 μL, which included 1 μL of extracted DNA, 1 μL (0.4 μM) of each primer, 22 μL of DNase-free water, and AccuPower ® PCR Premix.…”

Section: Pcr Analysis Of White Spot Syndrome Virus and Decapod Hepanh...mentioning

confidence: 99%

Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus

Lee,

Jeon,

Kim

et al. 2023

J World Aquaculture Soc

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In this study, Pacific white shrimp (Penaeus vannamei), pond water, crab, Helice tridens, and live food samples were collected from farms in three provinces of Korea to investigate the presence of white spot syndrome virus (WSSV) and decapod hepanhamaparvovirus (DHPV). By polymerase chain reaction (PCR), DHPV was detected in all shrimp samples (83 of 83); among these, 41 samples were detected with WSSV. Amplicon sequencing and phylogenetic analyses indicated that all DHPV samples were classified as a novel genotype of DHPV. Additionally, the collected squid, polychaeta, and crab were also confirmed to be detected with novel genotypes of DHPV. By WSSV quantitative PCR, the average concentrations of viruses detected in shrimp and pond water samples were 2.58 × 107 copies/μL and 1.27 × 102 copies/μL, respectively. Simultaneous detection of WSSV and DHPV was observed in shrimp, pond water, and crab samples, and co‐infection with both viruses in shrimp could be demonstrated by histopathological analysis. Viral infection can result in significant economic losses in shrimp production. Therefore, precise monitoring and management of WSSV and DHPV prevalence in pond water, live food, and habitats are essential to preventing the spread of viral diseases in the Korean shrimp culture industry.

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“…The most common shrimp pathogen detection methods include polymerase chain reaction (PCR), , in situ hybridization, antigen-antibody-based detection methods, and loop-mediated isothermal amplification (LAMP) . There has also been a shift toward the development of multiplex PCR assays, − which allow for the simultaneous detection of multiple shrimp pathogens in a single sample, saving time and reducing the cost of diagnostics.…”

Section: Introduction To Indian Aquaculture Settingmentioning

confidence: 99%

An Indian Perspective on the Infection and Diagnostic Landscape of Shrimp Aquaculture

Zwetlana

Tanwer

Evans

et al. 2023

ACS Agric. Sci. Technol.

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The shrimp aquaculture industry has seen substantial growth over the past two decades, which is driven by the increasing global demand for food. Farmed shrimp products are a highly valued commodity, with Indian market revenue from this industry alone being more than 10 billion USD. With this growth, the importance of addressing diseases caused by infectious agents such as bacteria, viruses, fungi, and protozoa has become increasingly important. Additionally, the mismanagement of antibiotics and the rise of antimicrobial resistance pose significant challenges in the industry. Efficient diagnostic solutions are needed to address these challenges and protect the sustainability of industry and consumer health. While traditional methods are used for detecting culturable pathogens, molecular diagnostics are still in their early stages of development. The high cost of molecular testing and the limited availability of laboratories are some of the challenges faced by aquaculture farmers. The development of rapid, sensitive, and deployable diagnostics and research tools at the farm and hatchery levels is essential for a better understanding and management of diseases and antimicrobial resistance. The importance of diagnostics in shrimp aquaculture cannot be overstated, as it is essential for the sustainability of the industry and protection of consumer health. In addition to continued research, industry must also focus on the commercialization of these technologies, as commercialized diagnostic solutions can offer a standardized and trustworthy method of disease management. This, in turn, can result in better disease control and a more sustainable shrimp aquaculture industry.

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Development of a multiplex PCR system for the simultaneous detection of white spot syndrome virus and hepatopancreatic parvovirus infection

Cited by 11 publications

References 57 publications

Prevalence and distribution of W hite S pot S yndrome V irus in cultured shrimp

Prevalence and distribution of W hite S pot S yndrome V irus in cultured shrimp

Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus

An Indian Perspective on the Infection and Diagnostic Landscape of Shrimp Aquaculture

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