Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus

Lei, Yun; Chen, Hongyu; Dai, Heping; Zeng, Zhaorui; Lin, Yi; Zhou, Feimeng; Pang, Dai‐Wen

doi:10.1016/j.bios.2007.10.004

Cited by 23 publications

(28 citation statements)

References 38 publications

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“…They measured the absorption spectra of the GNP monolayers in which GNPs were immobilized upon a glass slide by a functional organic coupling agent and found an optical response to polymers of different refractive indexes that were spin-cast on the immobilized GNPs [26]. Subsequently, a series of papers from different labs have presented this chip-based format optical biosensor, in which GNPs are immobilized on an optically transparent substrate [22,23,25,[45][46][47][48][49]. For instance, Matsui et al demonstrated a SPR sensor chip for detection of small molecules like dopamine [47].…”

Section: Gnp-based Optical Biosensorsmentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

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The unique properties of gold nanoparticles have stimulated the increasing interest in the application of GNPs in interfacing biological recognition events with signal transduction and in designing biosensing devices exhibiting novel functions. The optical properties of GNPs provide wide range opportunities for construction optical biosensors. The excellent biocompatibility, conductivity, catalytic properties and high surface-to-volume ratio and high density of GNPs facilitate extensive application of GNPs in construction of electrochemical and piezoelectric biosensors with enhanced analytical performance with respect to other biosensor designs. In this article, the recent advances in construction of GNP-based optical, electrochemical and piezoelectric biosensors are reviewed, and some illustrative examples given, with a focus on the roles GNPs play in the biosensing process and the mechanism of GNPs for improving the analytical performances. Finally, the review concludes with an outline of present and future research for the real-world applications.

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Section: Gnp-based Optical Biosensorsmentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

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“…The main methods reported for WSSV detection include: 1) the use of monoclonal and polyclonal antibodies [7-13], which has been described as simple, inexpensive and provides results considerably fast [14], but this method may be hampered by its low sensitivity [14,15]; 2) the use of biosensors [16-18], which have demonstrated a remarkably high sensitivity and a less elaborated sample preparation procedure. However, commercially available biosensors are often very expensive and require instrument-specific consumables [19]; 3) non-PCR molecular methods for WSSV DNA amplification [20-23], which, due to its basic principles of using four to six primers that recognize six to eight areas of the target sequence and the usage of the Bst DNA polymerase, offers the advantage of a high sensitivity and the synthesis of large amounts of amplicons under isothermal conditions.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a quantitative real-time polymerase chain assay for universal detection of the White Spot Syndrome Virus in marine crustaceans

Mendoza–Cano

Sánchez‐Paz

2013

Virol J

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BackgroundThe White Spot Syndrome Virus (WSSV), the sole member of the family Whispoviridae, is the etiological agent that causes severe mortality events in wild and farmed shrimp globally. Given its adverse effects, the WSSV has been included in the list of notifiable diseases of the Office of International Epizootic (OIE) since 1997. To date there are no known therapeutic treatments available against this lethal virus, and a surveillance program in brood-stock and larvae, based on appropriate diagnostic tests, has been strongly recommended. However, some currently used procedures intended for diagnosis of WSSV may be particularly susceptible to generate spurious results harmfully impacting the shrimp farming industry.MethodsIn this study, a sensitive one-step SYBR green-based real-time PCR (qPCR) for the detection and quantitation of WSSV was developed. The method was tested against several WSSV infected crustacean species and on samples that were previously diagnosed as being positive for WSSV from different geographical locations.ResultsA universal primer set for targeting the WSSV VP28 gene was designed. This method demonstrated its specificity and sensitivity for detection of WSSV, with detection limits of 12 copies per sample, comparable with the results obtained by other protocols. Furthermore, the primers designed in the present study were shown to exclusively amplify the targeted WSSV VP28 fragment, and successfully detected the virus in different samples regardless of their geographical origin. In addition, the presence of WSSV in several species of crustaceans, including both naturally and experimentally infected, were successfully detected by this method.ConclusionThe designed qPCR assay here is highly specific and displayed high sensitivity. Furthermore, this assay is universal as it allows the detection of WSSV from different geographic locations and in several crustacean species that may serve as potential vectors. Clearly, in many low-income import-dependent nations, where the growth of shrimp farming industries has been impressive, there is a demand for cost-effective diagnostic tools. This study may become an alternative molecular tool for a less expensive, rapid and efficient detection of WSSV.

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“…The system comprised of a polycarbonate microfluidic chip with an electrochemical detector, a gravity pump, and an automatic sample loading and injection unit. Lei et al [34] reported the analysis of prepared gold films with a surface plasmon resonance (SPR) device to detect the rapid and label-free detection of the white spot syndrome virus (WSSV). This method, key issues in the field of pisciculture and environmental toxicology, has been addressed and supplemented the expansion in the range of applications of the SPR technology.…”

Section: Recent Developments Of Electroless Goldmentioning

confidence: 99%

Electroless Deposition of Nanolayered Metallic Coatings

Sudagar¹,

Tamilarasan²,

Sanjith³

et al. 2017

Nanoscaled Films and Layers

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Electroless metallic coating is referred as the deposition of a substrate material by the process of chemical or autocatalytic reduction of aqueous metal ions deposited to a substrate material without any external supply of power. Electroless nickel alloys are generally considered synonymous to the word "electroless coating" as ~90% of productions in industries are of this alloy coating. Rest of the electroless metallic coatings includes gold, copper, palladium, cobalt, silver, etc. These electroless metallic coatings (other than electroless nickel coatings) are also one of the vibrant areas in the field of materials properties and surface engineering research. From the year 2000 to till date, nearly 1000 SCI indexed research papers were published on this topic. However, no comprehensive studies about the recent progress on this topic were reported elsewhere so far. In this context, the present chapter aims to give a complete overview on various aspects of the rest of the electroless metallic nanocoatings/layer as a whole. More importance will be on the recent developments of the nanocharacteristics and future scopes.

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Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus

Cited by 23 publications

References 38 publications

Gold nanoparticle-based biosensors

Gold nanoparticle-based biosensors

Development and validation of a quantitative real-time polymerase chain assay for universal detection of the White Spot Syndrome Virus in marine crustaceans

Electroless Deposition of Nanolayered Metallic Coatings

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