An aptamer-gated silica mesoporous material for thrombin detection

Oroval, Mar; Climent, Estela; Coll, Carmen; Eritja, Ramón; Aviñó, Anna; Marcos, M. Dolores; Sancenón, Félix; Martínez‐Máñez, Ramón; Amorós, Pedro

doi:10.1039/c3cc42157k

Cited by 87 publications

(50 citation statements)

References 43 publications

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“…The concentration of thrombin in blood varies considerably and can be virtually absent in healthy subjects. However, in the coagulation process, the concentration of thrombin in blood ranges from nM to low mM levels [3]. As the higher concentration of thrombin in blood is known to be relevant with some diseases [4], it is significant to be able to detect this enzyme accurately at trace levels.…”

Section: Introductionmentioning

confidence: 99%

A near-infrared fluorescent bioassay for thrombin using aptamer-modified CuInS2 quantum dots

Lin

Pan

et al. 2015

Microchim Acta

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We describe a near-infrared (NIR) fluorescent thrombin assay using a thrombin-binding aptamer (TBA) and Zn(II)-activated CuInS 2 quantum dots (Qdots). The fluorescence of Zn(II)-activated Q-dots is quenched by the TBA via photoinduced electron transfer, but if thrombin is added, it will bind to TBA to form G-quadruplexes and the Q-dots are released. As a result, the fluorescence intensity of the system is restored. This effect was exploited to design an assay for thrombin whose calibration plot, under optimum conditions, is linear in the 0.034 to 102 nmol L −1 concentration range, with a 12 pmol L −1 detection limit. The method is fairly simple, fast, and due to its picomolar detection limits holds great potential in the diagnosis of diseases associated with coagulation abnormalities and certain kinds of cancer.

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Section: Introductionmentioning

confidence: 99%

A near-infrared fluorescent bioassay for thrombin using aptamer-modified CuInS2 quantum dots

Lin

Pan

et al. 2015

Microchim Acta

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“…Those systems are able to deliver the entrapped cargo using several external stimuli such as light, [10][11] pH, [12][13] changes in redox potential, [14][15] temperature [16][17] and the presence of certain ions, molecules or biomolecules. [18][19][20] In particular, the design of gated mesoporous materials have proved to be a promising starting point for applying the versatility of molecular and supramolecular concepts to the design of gating solids, and a way of studying the factors that can influence the design of molecular gating functions with advanced delivery functionalities. These concepts contrast with the design of traditional delivery systems which are often based on simple diffusion controlled processes.…”

Section: Introductionmentioning

confidence: 99%

Polymer Composites Containing Gated Mesoporous Materials for On-Command Controlled Release

Acosta

Pérez‐Esteve

Fuenmayor

et al. 2014

ACS Appl. Mater. Interfaces

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Polyamidic nanofibrous membranes containing gated silica mesoporous particles, acting as carriers, are described as novel hybrid composite materials for encapsulation and oncommand delivery of garlic extracts. The carrier system consists of MCM-41 solids functionalized in the outer surface, with linear polyamines (Solid P1) and with hydrolyzed starch (Solid P2), both acting as molecular gates. Those particles were adsorbed on electospun nylon-6 nanofibrous membranes yielding to composite materials M1 and M2. FE-SEM analysis confirmed the presence of particles incorporated on the nylon nanofibers. The release of the entrapped molecules (garlic extract) from the P1, P2, M1 and M2 materials was evaluated using 2 cyclic voltammetry measurements. Electrochemical studies showed that at acidic pH P1 and M1 were unable to release their entrapped cargo (closed gate), whereas at neutral pH both materials release their loading (open gate). Dealing with P2 and M2 materials, in the absence of pancreatin a negligible release is observed (closed gate), whereas in the presence of enzyme the load is freely to diffuse to the solution. These newly developed composite nanomaterials, provide a homogeneous easy-to-handle system with controlled delivery and bioactive-protective features, having potential applications on pharmacology, medical and engineering fields INTRODUCTION

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“…This strategy harbors enormous potential for the development of novel signaling systems with enhanced features and it is highly flexible given the possible selection of different porous supports, diverse guest-selective binding sites (molecular gates) and a wide range of indicators. Following this gated approach hybrid sensory materials for the recognition of ATP, [38][39][40][41] long chain carboxylates, [27] methylmercury, [42] borate, [43] sulfathiazole, [44] oligonucleotides, [45] anionic surfactants, [46] nerve agent simulants, [47] finasteride, [48] nitroaromatic explosives, [49,50] mycoplasma, [51] thrombin, [52] TATP, [53] glucose, [54][55][56][57] adenosine [58] and certain cations [59][60][61] have been described recently.…”

Section: Introductionmentioning

confidence: 99%

Delivery modulation in silica mesoporous supports via functionalization in the pore outlets with a Zn(II)–bis(2-pyridylmethyl)amine complex

Bartovský

Ribes

Agostini

et al. 2014

Inorganica Chimica Acta

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ElsevierBartovsky ., P.; Ribes, À.; Agostini, A.; Benito Beorlegui, Á.; Martínez-Máñez, R. (2014). Delivery modulation in silica mesoporous supports via functionalization in the pore outlets with a Zn(II) bis(2-pyridylmethyl)amine complex. release. The delivery observed was clearly related with the ability of the anion to form complexes with the Zn(II)-dipicolylamine groups and with the size and charge of the anion.

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An aptamer-gated silica mesoporous material for thrombin detection

Cited by 87 publications

References 43 publications

A near-infrared fluorescent bioassay for thrombin using aptamer-modified CuInS2 quantum dots

A near-infrared fluorescent bioassay for thrombin using aptamer-modified CuInS2 quantum dots

Polymer Composites Containing Gated Mesoporous Materials for On-Command Controlled Release

Delivery modulation in silica mesoporous supports via functionalization in the pore outlets with a Zn(II)–bis(2-pyridylmethyl)amine complex

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