An aptamer assay for aflatoxin B1 detection using Mg2+ mediated free zone capillary electrophoresis coupled with laser induced fluorescence

Sun, Linlin; Li, Yapiao; Wang, Hailin; Zhao, Qiang

doi:10.1016/j.talanta.2019.05.069

Cited by 17 publications

(10 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of cations is fundamental to obtain a good separation of fluorescent aptamers and duplex DNA. Parameters such as the length of cDNA, concentrations of aptamers and cDNA, and their ratio must be carefully optimized together with the composition of BGE and the concentration of MgCl 2 added to the sample and run buffer (BGE) [ 42 ].…”

Section: Sample Preparation For Ce Analysismentioning

confidence: 99%

“…Considering OTA and AFs, OTA is a phenylalanine derivative and AFs have a tetrahydrocyclopenta[c]furo [3′,2′:4,5]furo[2,3-h]chromene skeleton. To supply to their low water solubility, mycotoxin solutions are prepared in organic solvents, such as acetonitrile [ 27 , 72 ] or methanol [ 56 , 58 ] or directly in neutral (pH 7.5) [ 42 , 67 ] and basic buffer (pH 8.5) [ 41 ].…”

Section: Ce Potentialities and Future Perspectivesmentioning

confidence: 99%

“…CE detection: The best detection system combined with CE remains LIF [ 27 , 41 , 42 , 44 , 67 , 72 ]. In particular, this detection is ideal for OTA and AFs, which have a native fluorescence.…”

Section: Ce Potentialities and Future Perspectivesmentioning

confidence: 99%

“…Polymerase Chain Reaction (PCR) protocols represent important routine techniques for the detection of potentially mycotoxigenic molds to prevent the mycotoxin presence in food matrices [ 37 ] and are associated with sensitive methods, such as HPLC-MS, CE, and particularly MEKC [ 38 , 39 ]. In addition, new offline and online/in-capillary pre-concentration techniques (in sample preparation step) or the use of sensitive detectors such as MS [ 36 , 40 ] and Laser-Induced Fluorescence (LIF) allowed food contaminants and residue analysis by CE [ 36 , 40 , 41 , 42 ]. Microchip-CE devices are mainly proposed when rapid analyses are needed to point out frauds and contaminations [ 36 , 43 , 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pre-Concentration and Analysis of Mycotoxins in Food Samples by Capillary Electrophoresis

Colombo

Papetti

2020

Molecules

View full text Add to dashboard Cite

Mycotoxins are considered one of the most dangerous agricultural and food contaminants. They are toxic and the development of rapid and sensitive analytical methods to detect and quantify them is a very important issue in the context of food safety and animal/human health. The need to detect mycotoxins at trace levels and to simultaneously analyze many different mycotoxin types became mandatory to protect public health. In fact, European Commission regulations specified both their limits in foodstuffs and official sample preparation protocols in addition to analytical methods to verify their presence. Capillary Electrophoresis (CE) includes different separation modes, allowing many versatile applications in food analysis and safety. In the context of mycotoxins, recent advances to improve CE sensitivity, particularly pre-concentration techniques or miniaturized systems, deserve remarkable attention, as they provide an interesting approach in the analysis of such contaminants in complex food matrices. This review summarizes the applications of CE combined with different pre-concentration approaches, which have been proposed in the literature (mainly) in the last ten years. A section is also dedicated to recent microchip–CE devices since they represent the most promising CE mode for this application.

show abstract

Section: Sample Preparation For Ce Analysismentioning

confidence: 99%

Section: Ce Potentialities and Future Perspectivesmentioning

confidence: 99%

Section: Ce Potentialities and Future Perspectivesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pre-Concentration and Analysis of Mycotoxins in Food Samples by Capillary Electrophoresis

Colombo

Papetti

2020

Molecules

View full text Add to dashboard Cite

show abstract

“…For example, G‐protein‐BODIPY FL GTPγS, hairpin‐structured DNA‐peptide nucleic acid, concanavalin A‐monosaccharides, dorzolamide‐human carbonicanhydrase II, and tubulin‐anti‐mitotic compounds pairs were examined . Other examples of the aptamer‐based ACE applications are summarized in Table .…”

Section: Aptamer‐based Affinity Cementioning

confidence: 99%

Capillary electrophoresis–based immunoassay and aptamer assay: A review

2020

View full text Add to dashboard Cite

Capillary electrophoresis-based immunoassay and aptamer assay: A reviewOver the last two decades, the group of techniques called affinity probe CE has been widely used for the detection and the determination of several types of biomolecules with high sensitivity. These techniques combine the low sample consumption and high separation power of CE with the selectivity of the probe to the target molecule. The assays can be defined according to the type of probe used: CE immunoassays, with an antibody as the probe, or aptamer-based CE, with an aptamer as the probe. Immunoassays are generally divided into homogeneous and heterogeneous groups, and homogeneous variant can be further performed in competitive or noncompetitive formats. Interacting partners are free in solution at homogeneous assay, as opposed to heterogeneous analyses, where one of them is immobilized onto a solid support. Highly sensitive fluorescence, chemiluminescence or electrochemical detections were typically used in this type of study. The use of the aptamers as probes has several advantages over antibodies such as shorter generation time, higher thermal stability, lower price, and lower variability. The aptamer-based CE technique was in practice utilized for the determination of proteins in biological fluids and environmentally or clinically important small molecules. Both techniques were also transferred to microchip. This review is focused on theoretical principles of these techniques and a summary of their applications in research.Abbreviations: Ag, antigen; CEIA, CE immunoassay; MCE, microchip CE; SELEX, systematic evolution of ligands by exponential enrichment and they are produced by the immune system of living organisms. Ag that start the immunological response are also called immunogens. These Ab-Ag complexes are stabilized by dipole-dipole, van der Waals or hydrophobic interactions and hydrogen bonds [5,6].The most often used immunoassays are ELISA with Ab or Ag immobilized onto solid support (plates, glass fibers, or plastic tubes), immunofluorescence assay, fluorescence polarization immunoassay, luminescence immunoassay, microparticle enzyme immunoassay, and biosensors. The major disadvantages of immunoassays are the tedious process, time-consuming reactions, and problems with reproducibility and nonspecific interactions [6].CE is a powerful analytical method for the separation of a wide range of molecules. The coupling of CE and immunoassay in the new CE immunoassay (CEIA) technique provided both separation power and specificity of interactions. Other advantages are the possibility of multianalyte analyses, automation, low sample consumption, high speed, flexibility, a lower amount of false-positive results, and also the possibility of connection with highly sensitive detectors [5].In CEIA, the Fab fragments of Ab containing Ag-specific regions are more often used than the whole monoclonal and Color online: See article online to view Figs. 4 and 7 in color.

show abstract

Recent trends in fluorescent aptasensors for mycotoxin detection in food: Principles, constituted elements, types, and applications

Yan

Chen

et al. 2022

Food Frontiers

View full text Add to dashboard Cite

Mycotoxins are the secondary metabolites of fungi in food and can be toxic to people. Therefore, developing simple, sensitive, and specific detection methods to detect mycotoxins in food is essential for ensuring food safety. Fluorescent aptasensors for the detection of mycotoxins in food have developed rapidly. They are simple and fast with high affinity and specificity. However, fluorescent aptasensors have not yet been applied in officially approved standards so far. This review elucidates fluorescent aptasensors for mycotoxin detection, including principles, constituted elements, types, and applications. Screening results of aptamers and each aptamer sequence used in fluorescent aptasensors are summarized to facilitate further research for later studies.We also focused on fluorescent probes, nanomaterials, and other strategies in the process of constructing fluorescent aptasensors. We then discuss current challenges and future perspectives in fluorescent aptasensors for mycotoxin detection.

show abstract

An aptamer assay for aflatoxin B1 detection using Mg2+ mediated free zone capillary electrophoresis coupled with laser induced fluorescence

Cited by 17 publications

References 40 publications

Pre-Concentration and Analysis of Mycotoxins in Food Samples by Capillary Electrophoresis

Pre-Concentration and Analysis of Mycotoxins in Food Samples by Capillary Electrophoresis

Capillary electrophoresis–based immunoassay and aptamer assay: A review

Recent trends in fluorescent aptasensors for mycotoxin detection in food: Principles, constituted elements, types, and applications

Contact Info

Product

Resources

About