An animal model of Fusarium solani endophthalmitis.

O’Day, Denis M.; Akrabawi, Patricia L.; Richmond, L D; Jones, Barrie R.; Clayton, Yvonne M.

doi:10.1136/bjo.63.4.277

Cited by 11 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increased rate of infectivity was observed with steroid pretreatment. Finally, O'Day et al (159) developed a rat model of fulminant ocular infection culminating in endophthalmitis that resembled remarkably that encountered in humans. F. solani was the infectious agent, and infection could be established and maintained without the need for immunosuppression.…”

Section: Pathogenesis Of Infectionmentioning

confidence: 99%

Taxonomy, biology, and clinical aspects of Fusarium species

1994

View full text Add to dashboard Cite

There are several taxonomic systems available for identifying Fusarium species. The philosophy used in each taxonomic system is discussed as well as problems encountered in working with Fusarium species in culture. Fusarium species are toxigenic, and the mycotoxins produced by these organisms are often associated with animal and human diseases. The implications for the association of the carcinogens, fumonisins, produced by Fusarium moniliforme and other Fusarium species with human diseases are discussed. Foreign-body-associated fusarial infection such as keratitis in contact lens wearers, onychomycosis, skin infections, and disseminated multiorgan infections are discussed. Disseminated fusarial hyalohyphomycosis has emerged as a significant, usually fatal infection in the immunocompromised host. Successful outcome is determined by the degree of immunosuppression, the extent of the infection, and the presence of a removable focus such as an indwelling central venous catheter. These infections may be clinically suspected on the basis of a constellation of clinical and laboratory findings, which should lead to prompt therapy, probably with one of the newer antifungal agents. Perhaps the use of such agents or the use of colony-stimulating factors may improve the outcome of this devastating infection. However, until new approaches for treatment develop, effective preventive measures are urgently needed.

show abstract

Section: Pathogenesis Of Infectionmentioning

confidence: 99%

Taxonomy, biology, and clinical aspects of Fusarium species

1994

View full text Add to dashboard Cite

show abstract

“…O'Day et al 4 injected Fusarium solani into the anterior chamber of outbread Wistar rats for the study of oculomycosis, while Fine and Zimmerman5 studied the ocular effectiveness of nystatin by intravitreal injection of Aspergillus fumigatus in albino rabbits.…”

mentioning

confidence: 99%

Development of quantitative methods of measuring antifungal drug effects in the rabbit cornea.

Oji¹

1981

British Journal of Ophthalmology

View full text Add to dashboard Cite

SUMMARY By means of multiple inoculation in each cornea with microtrephination a highly reproducible quantitative model of fungal infection of the rabbit corneal stroma has been produced. A known suspension of the chosen pathogen was systematically implanted into the trephine sites in the cornea. The degree of infectivity was monitored in both the preinoculation treated corneae (prophylaxis) and the postinoculation treated corneae (therapy). Examples measuring and comparing the antifungal effect of various imidazole drugs against Candida albicans are discussed.

show abstract

“…The potential virulence for ocular structures of F. solani, the most frequent pathogenic Fusarium species, has been confirmed in different animal models. The inoculation of this fungus into the anterior chambers of rats (14) or intravenously in mice (12) Although the in vitro antifungal susceptibility of F. polyphialidicum had not been previously tested, the low susceptibility shown by the case strain was partly expected because of the demonstrated resistance of Fusarium species to the available antifungal drugs (2,17). The high MIC of amphotericin B for this isolate correlated with the clinical outcome of the patient.…”

mentioning

confidence: 99%

Case of Keratitis Caused by an Uncommon Fusarium Species

Guarro

Rubio²,

Gené

et al. 2003

J Clin Microbiol

View full text Add to dashboard Cite

Fusarium polyphialidicum caused a corneal ulcer in a Spanish man. Diagnosis was established by a histopathological study and repeated cultures. The isolate was clearly resistant in vitro to the antifungal agents tested. This is the first case of human or animal mycosis by this rare fungus. CASE REPORTA 65-year-old Spanish male was referred to the Ophthalmology Service of the Lozano Blesa University Hospital in Zaragoza on 5 June 2000 because of acute keratitis in his left eye. Approximately 1 month earlier, the patient suffered a nonpenetrating foreign-body injury when he was working on his farm. Since then, he had complained of increasing disturbance in the eye. On examination, he showed a central corneal ulcer of ca. 4 mm in diameter with irregular borders, slight infiltration, and a thinned weakness but with no risk of perforation. A hypopyon 1.2 mm high was also observed. Visual acuity was 1 to 200. The right eye showed no abnormality. Results of other local and systemic examinations were negative.Deep corneal scrapings were collected with a sterile scalpel blade for fungal and bacteriological cultures. The corneal scrapings were inoculated directly onto Sabouraud glucose agar (Oxoid, Basingstoke, England) and incubated at 25 and 37°C. After 2 days, numerous small whitish colonies appeared on all cultures. All the colonies were apparently identical, and the fungus was identified as Fusarium sp. The results of routine bacteriological cultures were negative. Since the fungus was detected, treatment was initiated with 0.5% amphotericin B applied topically every hour. However, after a week, the condition of the eye worsened; the corneal infiltration and hypopyon increased. The ulcer became torpid and enlarged into a deepening corneal abscess and endophthalmitis. More corneal scrapings were collected for new cultures, which were again negative for bacteria and positive for the same fungus recovered previously. A corneal transplant was performed on 30 June. Cultures from a biopsy of the excised cornea again yielded the same Fusarium sp. Portions of the cornea were fixed in 10% formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin. Sections of the stained tissue revealed short, contorted, hyaline, septate, hyphal fragments (Fig. 1). The transplanted eye improved considerably, and therefore the patient was discharged. In September 2000, the patient presented a visual acuity of 20 to 40 in the affected eye.The clinical strain was sent to the Faculty of Medicine of the Rovira i Virgili University in Reus, Spain, to be identified and to determine its antifungal susceptibility.Mycological study and diagnosis. For identification purposes, the fungus was subcultured on potato dextrose agar (PDA; Difco Laboratories, Detroit, Mich.) and oatmeal agar (30 g of oat flakes, 1 g of MgSO 4 ⅐ 7H 2 O, 1.5 g of KH 2 PO 4 , 15 g of agar, 1,000 ml of tap water) and incubated at 25, 37, and 40°C in darkness. After 12 days at 25°C, the colonies on PDA attained a diameter of 68 to 70 mm and those on oatmeal ag...

show abstract

An animal model of Fusarium solani endophthalmitis.

Cited by 11 publications

References 3 publications

Taxonomy, biology, and clinical aspects of Fusarium species

Taxonomy, biology, and clinical aspects of Fusarium species

Development of quantitative methods of measuring antifungal drug effects in the rabbit cornea.

Case of Keratitis Caused by an Uncommon Fusarium Species

Contact Info

Product

Resources

About