Development of quantitative methods of measuring antifungal drug effects in the rabbit cornea.

Oji, Erasmus O.

doi:10.1136/bjo.65.2.89

Cited by 12 publications

(1 citation statement)

References 5 publications

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“…evaluate antifungal agents10, 11,12,13). However, the mechanisms of adhesion and invasion of the pathogenic fungi to the cornea have not yet been analyzed, and an analysis of the corneal infection of Candida would be helpful in an understanding of the mechanism and control of fungal keratitis.…”

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confidence: 99%

Pathogenicity of Candida albicans strains with different proteinase activity in the rabbit cornea.

et al. 1989

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Four strains of Candida albicans with different abilities in secreting proteinase in culture were inoculated into rabbit cornea and the severity of the infection was compared by histopathological examination. Generally, the proteinase-secreting strains developed more severe and extensive infections in the cornea than the proteinase-deficient ones. Our results on the comparative studies of a proteinase-producing 9615 with its variant 961VGR4, which had lost the ability of proteinase secretion, in the corneal tissue invasion suggested that proteinase worked to enhance or continue the corneal infection against the host defence mechanism.

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confidence: 99%

Pathogenicity of Candida albicans strains with different proteinase activity in the rabbit cornea.

et al. 1989

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Animal Models for Candidiasis

Guentzel

Cole²,

Pope³

1985

Current Topics in Medical Mycology

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Ketoconazole: a new imidazole antifungal agent has both prophylactic potential and therapeutic efficacy in keratomycosis of rabbits

Oji

1982

Int Ophthalmol

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A highly reproducible quantitative model of fungal infection of the rabbit's corneal stromal was produced using multiple corneal microtrephination. Aspergillus flavus (K4/77), at a concentration of 10(6) spores per ml was systematically implanted into the trephine sites in the cornea, and the degree of corneal infection determined. By pre-inoculation and post-inoculation challenge of these cornea with 1% ketoconazole in arachis oil, the prophylactic potential and the therapeutic usefulness of ketoconazole was determined. Ketoconazole, acetyl-dichlorophenyl-imidazole, has a significant prophylactic potential in inhibiting the development of corneal stromal fungal lesions when it is administered to the cornea of New Zealand white male rabbits as a 1% solution in arachis oil for two consecutive times hourly for two hours before the inoculation of the rabbits cornea with an ocular pathogenic Aspergillus flavus. Ketoconazole also has a therapeutic effect in the reduction of well established A. flavus keratitis in rabbits. When administered as 1% solution in arachis oil for ten consecutive hours daily to well established A. flavus lesions of the cornea of New Zealand albino rabbits, ketoconazole took about sixteen days to cure all the corneal lesions. Finally, using a yeast nitrogen base liquid medium, the in vitro minimal inhibitory concentrations of ketoconazole to twenty-five various human ocular pathogenic fungal isolates were determined and used to recommend those fungi for which ketoconazole would be a good choice for therapy.

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Development of quantitative methods of measuring antifungal drug effects in the rabbit cornea.

Cited by 12 publications

References 5 publications

Pathogenicity of Candida albicans strains with different proteinase activity in the rabbit cornea.

Pathogenicity of Candida albicans strains with different proteinase activity in the rabbit cornea.

Animal Models for Candidiasis

Ketoconazole: a new imidazole antifungal agent has both prophylactic potential and therapeutic efficacy in keratomycosis of rabbits

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