An analgetic activity of sedges (Cyperus rotundus L.) extract on white male mice (Mus musculus L.)

Abstract

The objectives of the research were to find out the effect of giving sedges root extract orally on the number of writhing after chemical pain induction and time reaction after thermal pain induction of mice and also to find out the extract dosage which had an influence on decreasing number of writhing after chemical pain induction and length of reaction time after thermal pain induction of mice. The Complete Random Design (CRD) with 6 treatment groups and each treatment used 5 repetitions were used in this study. The groups were: Group I , control group, treated with sedges root extract of 0 mg/ 20 g BW , 0,5 ml; Group II treated with sedges root extract of 1 mg/ 20 g BW, 0,5 ml; Group III treated with sedges root extract of 3 mg/ 20 g BW, 0,5 ml; Group IV treated with sedges root extract of 5 mg/ 20 g BW, 0,5 ml; Group V treated with sedges root extract of 7 mg/ 20 g BW, 0,5 ml; Group VI treated with asetosal 200 mg/ kg BW , 0,5 ml/ 20 g BW and for the activity test, the sedges root extract was suspended in CMC 1%. Induction of chemical pain was done according to Witkin et al. (1965) in Pudjiastuti et al. (2000), in which 0,1 ml 3% of Acetic Acid/ 20 g BB was injected intraperitoneally 30 minutes after giving oral-material test. The mouse gave a respond in the way of writhing. Thermal pain induction was done by placing the mouse on hot plate with constant temperature of 55oC. The mouse gave a respond in the way of lick its back foot or even jumping. The data collected was analyzed using one direction ANOVA model and it was continued with LSD test in order to find out the difference every treatment group. The result of the analysis showed that the sedges root extract dosage of 7 mg/ 20 g BB decreased the number of writhing after chemical pain induction and length of mouse time reaction after thermal pain induction, so that sedges root extract dosage 7 mg/ 20 g BB had an analgetic function.

ANALGETIC ACTIVITY TEST OF ETHANOL EXTRACT SUNGKAI LEAF (Peronema canescens Jack) IN WHITE MALE MOUSE (Mus musculus) INDUCED WITH ACETIC ACID

“…From the data in the Table 1, it can be seen that the three doses of Sungkai leaf ethanol extract and the positive control (paracetamol) showed that there was a difference in the mean total stretchiness compared to the positive control of 11; 13.8; 12.2; and 8.4 while with a negative control of 27.6. The less the average number of stretches obtained, the better the analgesic effect (Puspitasari et al, 2023;Arifin et al, 2018).…”

“…An inverse comparison between the mean number of stretches and the percentage of analgetic protection in the test section against the negative control means that the more total stretches the mean, the smaller the percentage of analgetic protection obtained and vice versa (Puspitasari et al, 2023).…”

Characterization of microencapsulated Saga Leaves Extract (Abrus precatorius L.) and Analgetic Activity Tests in Male Mice (Mus musculus)

“…F2 and F3 microcapsules are more than 50%. according to Puspitasari et al (2003). it was stated that they had analgesic activity.…”

Morphology, biology, and damage behaviors of nutsedge borer (Bactra venosana Zeller) in Tra Vinh Province, Vietnam

“…Several species in this family are used in folk medicine (Hibi et al 2019). The studies stated that the extract of the Cyperus rotundus root had an analgesic effect on mice, and its rhizosphere contains bacteria with antimicrobial abilities (Ambarwati et al 2019;Puspitasari et al 2003). Cyperus malaccensis, which has become a vital fiber crop and grown in Japan and China, is the raw material for making mats (Shioya et al 2019).…”