An amperometric glucose biosensor based on PEDOT nanofibers

Abstract

Here we present a simple, low cost approach for the production of PEDOT nanofiber biosensors using simple techniques. Firstly, nanofibers of PEDOT were produced by the chemical vapor polymerization of EDOT on FeCl 3 containing electrospun PAN nanofiber mats. The nanofibers were characterized by SEM, FTIR, CV and conductivity studies, which indicated the formation of homogeneous, porous, electroactive PEDOT nanofibers. The fabrication of biosensors was achieved through the loading of various amounts of GOx on the nanofibers. To uncover their capability, the biosensors were operated under both hydrogen peroxide production and oxygen consumption conditions. For each biosensor current response versus glucose concentration calibration curves were plotted. The sensitivity, linear range, LOD, K m and I max values of the biosensors were determined and the stabilities of all the sensors were investigated. The biosensor operating at 0.6 V revealed a lower LOD with a wider linear range, higher stability, good sensitivity and selectivity. For example, the PEDOT-NFs/GOx-3 nanofiber biosensor showed good sensitivity (74.22 mA mM À1 cm À2 ) and LOD (2.9 mM) with a response time of 2-3 s without any interference effects. The PEDOT-NFs/GOx-2 biosensor operating at À0.6 V exhibited extreme sensitivity of 272.58 mA mM À1 cm À2. Our studies have shown that having good sensitivity, LOD and stability makes these interference-free, easy to construct sensors viable candidates for commercialization.

Custom-Made Ion Exchange Membranes at Laboratory Scale for Reverse Electrodialysis

“…In Figure 2 we present the FTIR spectrum of the different reagents used to synthesize the AEMs before and after modification. First, the spectrum of PECH and PAN are presented with their principal peaks [53,54] (panels a and b), followed by the synthesized AEM in panel c. The three characteristic peaks verify the proper crosslinking of the polymers above the first peak, derived from the PECH, corresponds to the OH group at 3378 cm −1 ; the second peak, at 2242 cm −1 belongs to the C-N of the PAN polymer; lastly, the third peak at 1640 cm −1 is associated with the C-N group that also corresponds to the PECH polymer [39]. This membrane was superficially modified immersing it alternately in PEI and GA solutions.…”

Deposition of nanomaterials: A crucial step in biosensor fabrication

“…Notably, the direct electrodeposition of composite nanomaterials has been preferred for realizing the high conductivity and synergistic effect between the composite materials for biosensing applications. However, to explore the Copyright 2018 Elsevier) polymerization of EDOT and deposited it onto an electrode surface by electrospinning followed by GOx loading in various amounts [265]. This study showed that the excessive GOx loading on PEDOT nanofibers had an adverse effect on response time due to excessive swelling of the PEDOT matrix.…”

Electroactive Smart Polymers for Biomedical Applications

“…However, for biomedical applications, their use is limited mainly because of their poor processability and mechanical properties [36]. The doping of these polymers with long chains can overcome these limitations although it can affect the conductivity of the resulting materials [34,42]. Another solution is to blend the intrinsic conductive polymer with another polymer possessing easier processability in order to obtain a composite with improved mechanical and biocompatibility properties [30].…”