An agonist for membrane progestin receptor (mPR) induces oocyte maturation and ovulation in zebrafish in vivo

Rezanujjaman,; Tanvir, Razain; Ali, Hasan; Tokumoto, Toshinobu

doi:10.1016/j.bbrc.2020.05.208

Cited by 11 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the initial studies of mPR discovery, Org OD 02 was already discovered as an mPR-specific agonist and has been used for research purposes 11,14 . We have also shown that Org has the ability to induce oocyte maturation in fish 15 . The experimental systems for in vitro oocyte maturation in fish and amphibians have long been shown to be induced by nongenomic actions of progesterone, and were have been used as a test for nongenomic actions, leading to the discovery of mPR 5 .…”

Section: Introductionmentioning

confidence: 53%

See 1 more Smart Citation

Discovery of specific activity of 2-HPA acting on the membrane progestin receptor α (paqr7) by purification of natural products from the marine algaePadina

Amin,

Acharjee,

Sarwar Jyoti

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Membrane progestin receptors (mPRs) are members of the progestin and adipoQ (PAQR) receptor family that are stimulated by endogenous steroids to initiate rapid intracellular signalling through a nongenomic pathway. Previously, water-soluble compounds with mPRα-binding activity from the marine algaePadina arborescenswere fractionated by HPLC steps. In this study, the structure of one of the major compounds in the fraction was identified as 2-hydroxypentanoic acid (2-HPA) using Nuclear Magnetic Resonance spectroscopy. 2-HPA showed a substantial competitive binding affinity for hmPRα in the GQD-hmPRα binding assay. In contrast, synthetic structural analogues of 2-HPA showed no competitive binding activity. The physiological activity of 2-HPA and its analogues was then investigated usingin vitrogoldfish andin vivozebrafish oocyte maturation and ovulation assays. As with the hmPRα binding assay, only 2-HPA showed inhibitory activity on oocyte maturation and ovulation of fish oocytes. Furthermore, the inhibitory activity of 2-HPA was compared between S- and R-type 2-HPA. The results showed that both types had the same level of activity. These results indicate that 2-HPA, found as a secreted compound fromPadina arborescens, is a novel mPRα antagonist and its chemical structure is highly restricted to show its activity.

show abstract

Section: Introductionmentioning

confidence: 53%

“…An in vitro and in vivo zebrafish oocyte maturation and ovulation assay was performed as described previously 15,18 .…”

Section: Methodsmentioning

confidence: 99%

Discovery of specific activity of 2-HPA acting on the membrane progestin receptor α (paqr7) by purification of natural products from the marine algaePadina

Amin,

Acharjee,

Sarwar Jyoti

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…An in vitro and in vivo oocyte maturation and ovulation assay was conducted in zebrafish as described previously (Rezanujjaman et al 2020).…”

Section: In Vitro and In Vivo Oocyte Maturation Assaymentioning

confidence: 99%

The antagonistic activity of Padina arborescens extracts on mPRα

Acharjee

Ali

Jyoti

et al. 2022

Natural Product Research

Self Cite

View full text Add to dashboard Cite

The current study attempted to evaluate the antagonistic activity of compounds isolated and purified from the marine algae Padina arborescens during cultivation. The compounds were collected on a filter, concentrated on ODS columns and separated by HPLC. Two peaks that showed competitive progesterone binding activity with membrane progesterone receptor α (mPRα) were purified. Their physiological activity was further uncovered by in vitro and in vivo oocyte maturation and ovulation-inducing assays using zebrafish. The compounds inhibited the induction of oocyte maturation and ovulation. Moreover, the results showed that the compounds have antagonistic activity against mPRα. The purified compounds with antagonistic activity against mPRα would be considered as new pharmaceutical candidate.

show abstract

“…If the involvement of nuclear progestogen receptors in the FOM remains to be demonstrated in fish (Hanna et al, 2009;Tang et al, 2016), appealing to transactivation studies of these receptors after appropriate transfection in cell lines may be a future guide to discover the most potent endogenous agonists to activate these receptors (Hanna et al, 2009). Once the profile of progestogen receptors involved in FOM is validated, pharmacological approaches using chemicals as agonists or antagonists of these receptors can also be useful to define the chemical structure of the active ligands and thus to propose potential MIH (Rezanujjaman et al, 2020;Tang et al, 2016).…”

Section: Characterization Of the Ligand-receptors Interactionsmentioning

confidence: 99%

Maturation Inducing Hormones in teleosts: Are progestogens always the first to be nominated?

et al. 2022

View full text Add to dashboard Cite

An agonist for membrane progestin receptor (mPR) induces oocyte maturation and ovulation in zebrafish in vivo

Cited by 11 publications

References 24 publications

Discovery of specific activity of 2-HPA acting on the membrane progestin receptor α (paqr7) by purification of natural products from the marine algaePadina

Discovery of specific activity of 2-HPA acting on the membrane progestin receptor α (paqr7) by purification of natural products from the marine algaePadina

The antagonistic activity of Padina arborescens extracts on mPRα

Maturation Inducing Hormones in teleosts: Are progestogens always the first to be nominated?

Contact Info

Product

Resources

About