The antagonistic activity of <i>Padina arborescens</i> extracts on mPRα

Acharjee, Mrityunjoy; Ali, Md. Hasan; Jyoti, Md. Maisum Sarwar; Rezanujjaman, Md.; Hassan, Md. Maksudul; Rana, Md. Rubel; Hossain, Md. Forhad; Kodani, Shinya; Tokumoto, Toshinobu

doi:10.1080/14786419.2022.2120873

Cited by 5 publications

(4 citation statements)

References 27 publications

(2 reference statements)

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“…Sampling and HPLC purification were carried out as previously described 24 . Two peaks (Peak1 and 2) were obtained in the final purification step.…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, we are also trying to isolate and purify mPR-reactive natural products using these screening methods 22 . We have discovered the presence of mPR-reactive substances in the seawater of coral reefs in Mauritius and recently succeeded in separating them into two peaks 23,24 . In this study, the chemical structure of the main component of one of these two peaks was successfully determined.…”

Section: Introductionmentioning

confidence: 99%

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Discovery of specific activity of 2-HPA acting on the membrane progestin receptor α (paqr7) by purification of natural products from the marine algaePadina

Amin,

Acharjee,

Sarwar Jyoti

et al. 2023

Preprint

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Membrane progestin receptors (mPRs) are members of the progestin and adipoQ (PAQR) receptor family that are stimulated by endogenous steroids to initiate rapid intracellular signalling through a nongenomic pathway. Previously, water-soluble compounds with mPRα-binding activity from the marine algaePadina arborescenswere fractionated by HPLC steps. In this study, the structure of one of the major compounds in the fraction was identified as 2-hydroxypentanoic acid (2-HPA) using Nuclear Magnetic Resonance spectroscopy. 2-HPA showed a substantial competitive binding affinity for hmPRα in the GQD-hmPRα binding assay. In contrast, synthetic structural analogues of 2-HPA showed no competitive binding activity. The physiological activity of 2-HPA and its analogues was then investigated usingin vitrogoldfish andin vivozebrafish oocyte maturation and ovulation assays. As with the hmPRα binding assay, only 2-HPA showed inhibitory activity on oocyte maturation and ovulation of fish oocytes. Furthermore, the inhibitory activity of 2-HPA was compared between S- and R-type 2-HPA. The results showed that both types had the same level of activity. These results indicate that 2-HPA, found as a secreted compound fromPadina arborescens, is a novel mPRα antagonist and its chemical structure is highly restricted to show its activity.

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“…Sampling and HPLC purification were carried out as previously described 24 . Two peaks (Peak1 and 2) were obtained in the final purification step.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Discovery of specific activity of 2-HPA acting on the membrane progestin receptor α (paqr7) by purification of natural products from the marine algaePadina

Amin,

Acharjee,

Sarwar Jyoti

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sampling and HPLC puri cation were carried out as previously described 24 . Two peaks (Peak1 and 2) were obtained in the nal puri cation step.…”

Section: Separation Of Components In Padina Secretions By Hplcmentioning

confidence: 99%

Discovery of specific activity of 2-HPA acting on the membrane progestin receptor alpha (paqr7) by purification of natural products from the marine algae Padina

Amin,

Acharjee,

Jyoti

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Membrane progestin receptors (mPRs) are members of the progestin and adipoQ (PAQR) receptor family that are stimulated by endogenous steroids to initiate rapid intracellular signalling through a nongenomic pathway. Previously, water-soluble compounds with mPRα-binding activity from the marine algae Padina arborescens were fractionated by HPLC steps. In this study, the structure of one of the major compounds in the fraction was identified as 2-hydroxypentanoic acid (2-HPA) using Nuclear Magnetic Resonance spectroscopy. 2-HPA showed a substantial competitive binding affinity for hmPRα in the GQD-hmPRα binding assay. In contrast, synthetic structural analogues of 2-HPA showed no competitive binding activity. The physiological activity of 2-HPA and its analogues was then investigated using in vitro goldfish and in vivo zebrafish oocyte maturation and ovulation assays. As with the hmPRα binding assay, only 2-HPA showed inhibitory activity on oocyte maturation and ovulation of fish oocytes. Furthermore, the inhibitory activity of 2-HPA was compared between S- and R-type 2-HPA. The results showed that both types had the same level of activity. These results indicate that 2-HPA, found as a secreted compound from Padina arborescens, is a novel mPRα antagonist and its chemical structure is highly restricted to show its activity.

show abstract

“…Also, finding of other receptors for progestins it raises the necessity of analysis of ligand binding specificity assay on different receptors [ 19 ]. In fact, recently we have purified natural compounds that act on mPRα from seaweed [ 20 ]. It is necessary to analyze the reactivity of these compounds with Pgr.…”

Section: Introductionmentioning

confidence: 99%

The antagonistic activity of Padina arborescens extracts on mPRα

Cited by 5 publications

References 27 publications

Discovery of specific activity of 2-HPA acting on the membrane progestin receptor α (paqr7) by purification of natural products from the marine algaePadina

Discovery of specific activity of 2-HPA acting on the membrane progestin receptor α (paqr7) by purification of natural products from the marine algaePadina

Discovery of specific activity of 2-HPA acting on the membrane progestin receptor alpha (paqr7) by purification of natural products from the marine algae Padina

Establishment of a graphene quantum dot (GQD) based steroid binding assay for the nuclear progesterone receptor (pgr)

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