Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications

Kanyong, Prosper; Hughes, Gareth; Pemberton, R. M.; Jackson, Simon K.; Hart, John P.

doi:10.1080/00032719.2015.1070166

Cited by 17 publications

(14 citation statements)

References 31 publications

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“…In a subsequent report, a microband galactose biosensor [21] was applied to the determination of galactose taken up by hepatocellular carcinoma cells (HepG2). In order to measure the toxicity of paracetamol to HepG2 cells, the cells were incubated with 10 mM galactose and different concentrations of paracetamol for 24 h. The enzyme was immobilised utilizing a similar method.…”

Section: Screen-printed Carbon Based Biosensors For the Determinatmentioning

confidence: 99%

Recent Advances in the Fabrication and Application of Screen-Printed Electrochemical (Bio)Sensors Based on Carbon Materials for Biomedical, Agri-Food and Environmental Analyses

et al. 2016

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This review describes recent advances in the fabrication of electrochemical (bio)sensors based on screen-printing technology involving carbon materials and their application in biomedical, agri-food and environmental analyses. It will focus on the various strategies employed in the fabrication of screen-printed (bio)sensors, together with their performance characteristics; the application of these devices for the measurement of selected naturally occurring biomolecules, environmental pollutants and toxins will be discussed.

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Section: Screen-printed Carbon Based Biosensors For the Determinatmentioning

confidence: 99%

Recent Advances in the Fabrication and Application of Screen-Printed Electrochemical (Bio)Sensors Based on Carbon Materials for Biomedical, Agri-Food and Environmental Analyses

et al. 2016

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“…So far, the most common method for the Gal analysis is the enzyme-based techniques using two types of enzymes: galactose oxidase (GalO x ) or galactose dehydrogenase (GADH), in association with spectrophotometric, polarimetric, and fluorometric detection of enzymatic products [8,9]. The GADH technique has been developed and commercialized; however, it is still expensive, time-consuming, and inconvenient (requires skilled personnel to operate them) [8][9][10]. Thus, electrochemical measurements, especially in conjunction with the use of amperometric biosensors based on GalO x , are becoming more attractive for Gal analysis [11].…”

Section: Introductionmentioning

confidence: 99%

The Facile Synthesis of Novel ZnO Nanostructure for Galactose Biosensor Application

Phan

Tran

Dinh

et al. 2019

Journal of Nanomaterials

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We introduce a novel structure of ZnO nanorods (NRs) grown on ZnO NRs (ZnO NRs/NRs) via a facile, low-cost, and environmentally friendly synthesis for galactose biosensor application. The galactose oxidase enzyme (GalO x ) is immobilized on the ZnO NR/NR surface to form the novel electrode structure (GalO x |ZnO NRs/NRs). The GalO x |ZnO NR/NR electrode has a linear detection range of current density from 11.30 μA/mm 2 to 18.16 μA/mm 2 over a galactose concentration range from 40 mM to 230 mM, indicating the increment of electrode sensitivity up to 60.7%. The ZnO NR/NR morphology with a high surface area to volume ratio has a great contribution to the electrochemical performance of galactose biosensor. Our results propose a straightforward approach to fabricate architecturally ZnO-based nanostructure for biosensor application.

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“…Even though these methods are sensitive towards catechol, they are usually not only time-consuming, laborious, and require skilled-personnel to operate, but also involve complicated operational procedures that makes them unsuitable for point-of-need applications. Owing to the electroactive nature of catechol, the use of electrochemical techniques, especially at modified electrodes, are most attractive because they give rapid response and are simple, relatively inexpensive, selective, and sensitive [ 12 , 13 , 14 , 15 ]. Different nanomaterials including carbon nanomaterials, nanoparticles, metals and metal oxides, conducting polymers [ 16 , 17 , 18 , 19 ] including electrode pre-treatments and/or modifications have been developed for the quantification of catechol [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

A Disposable Amperometric Sensor Based on High-Performance PEDOT:PSS/Ionic Liquid Nanocomposite Thin Film-Modified Screen-Printed Electrode for the Analysis of Catechol in Natural Water Samples

Krampa

Aniweh

Awandare

et al. 2017

Sensors

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A conducting polymer-based composite material of poly(3,4-ethylenedioxythiophene) (PEDOT): poly(4-styrenesulfonate) (PSS) doped with different percentages of a room temperature ionic liquid (IL), 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIM][BF4]), was prepared and a very small amount of the composite (2.0 µL) was drop-coated on the working area of a screen-printed carbon electrode (SPCE). The SPCE, modified with PEDOT:PSS/IL composite thin-film, was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), profilometry and sessile contact angle measurements. The prepared PEDOT:PSS/IL composite thin-film exhibited a nano-porous microstructure and was found to be highly stable and conductive with enhanced electrocatalytic properties towards catechol, a priority pollutant. The linear working range for catechol was found to be 0.1 µM–330.0 µM with a sensitivity of 18.2 mA·mM·cm−2 and a calculated limit of detection (based on 3× the baseline noise) of 23.7 µM. When the PEDOT:PSS/IL/SPCE sensor was used in conjunction with amperometry in stirred solution for the analysis of natural water samples, the precision values obtained on spiked samples (20.0 µM catechol added) (n = 3) were 0.18% and 0.32%, respectively, with recovery values that were well over 99.0%.

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Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications

Cited by 17 publications

References 31 publications

Recent Advances in the Fabrication and Application of Screen-Printed Electrochemical (Bio)Sensors Based on Carbon Materials for Biomedical, Agri-Food and Environmental Analyses

Recent Advances in the Fabrication and Application of Screen-Printed Electrochemical (Bio)Sensors Based on Carbon Materials for Biomedical, Agri-Food and Environmental Analyses

The Facile Synthesis of Novel ZnO Nanostructure for Galactose Biosensor Application

A Disposable Amperometric Sensor Based on High-Performance PEDOT:PSS/Ionic Liquid Nanocomposite Thin Film-Modified Screen-Printed Electrode for the Analysis of Catechol in Natural Water Samples

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