We introduce a novel structure of ZnO nanorods (NRs) grown on ZnO NRs (ZnO NRs/NRs) via a facile, low-cost, and environmentally friendly synthesis for galactose biosensor application. The galactose oxidase enzyme (GalO x ) is immobilized on the ZnO NR/NR surface to form the novel electrode structure (GalO x |ZnO NRs/NRs). The GalO x |ZnO NR/NR electrode has a linear detection range of current density from 11.30 μA/mm 2 to 18.16 μA/mm 2 over a galactose concentration range from 40 mM to 230 mM, indicating the increment of electrode sensitivity up to 60.7%. The ZnO NR/NR morphology with a high surface area to volume ratio has a great contribution to the electrochemical performance of galactose biosensor. Our results propose a straightforward approach to fabricate architecturally ZnO-based nanostructure for biosensor application.
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