Amperometric aptasensor for carcinoembryonic antigen based on the use of bifunctionalized Janus nanoparticles as biorecognition-signaling element

Paniagua, Gonzalo; Villalonga, Anabel; Eguílaz, Marcos; Vegas, Borja; Parrado, Concepción; Rivas, Gustavo A.; Díez, Paula; Villalonga, Reynaldo

doi:10.1016/j.aca.2019.02.015

Cited by 51 publications

(31 citation statements)

References 43 publications

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“…In Step B, amperometric signals obtained without CEA (signal‐OFF). The designed assembly successfully detected CEA biomarker within a linear range of 1–5000 ng/mL with a LOD of 1.2 pM in serum samples [39].…”

Section: Aptasensors For Biological Analytes Detectionmentioning

confidence: 99%

Electrochemical Aptasensors for Biological and Chemical Analyte Detection

Topkaya

Çetin²

2020

Electroanalysis

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Aptamers are short length, single‐stranded DNA or RNA affinity molecules which interact with any desired targets such as biomarkers, cells, biological molecules, drugs or chemicals with high sensitivity. They have been extensively employed for medical applications due to having more advantages than the antibodies such as easier preparation and modification, higher stability, lower batch‐to‐batch variability and cost. Moreover, aptamers can be easily integrated efficiently with sensors, biosensors, actuators and other devices. In this review article, different applications of aptamers for biological and chemical molecules detection within the scope of electrochemical methods were presented with recent studies. In addition, the present status and future perspectives for highly‐effective aptasensors for specific and selective analyte detection were discussed. As in stated throughout the review, combining of extraordinary properties of aptamers with the electrochemical‐based biosensors could have improved the sensitivity of the assay and reduced limit of detection.

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Section: Aptasensors For Biological Analytes Detectionmentioning

confidence: 99%

Electrochemical Aptasensors for Biological and Chemical Analyte Detection

Topkaya

Çetin²

2020

Electroanalysis

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“…Moreover, Janus nanoparticles may incorporate a magnetic material to allow magnetic driving [43,44]. Regarding biosensing, these unique nanoparticles have been exploited as transducer modifiers [47,48] and advanced nanocarriers of signaling molecules for signal amplification [49,50].…”

Section: Aunps Aunws Janus and Magnetic Nanoparticles: Synthesismentioning

confidence: 99%

“…Janus Au-mesoporous silica nanoparticles (Au-MS JNPs) were previously exploited as scaffolds to design integrated electrochemical enzymatic biosensors [47,48]. Recently, they have also been used as labels for signal amplification in the electrochemical aptasensing of carcinoembryonic antigen (CEA) [49]. The strategy involved proper functionalization of Janus nanoparticles silica surfaces with HRP to act as signaling element while the Au face was modified with a dual biotin thiol-functionalized anti-CEA DNA hairpin aptamer (Figure 7a top).…”

Section: Biosensing and Delivery Of Nucleic Acids: Roles Opportunmentioning

confidence: 99%

“…This resulted in an enhanced amperometric signal in the presence of H 2 O 2 /HQ (hydroquinone) upon their magnetic capture on the working electrode of SPCEs (Figure 7a down). This aptasensor was able to detect CEA in the range from 5.5 pM to 28 nM, with a LOD of 1.2 pM and was applied to the analysis of spiked lyophilized human serum [49]. A closely related method has been proposed recently by the same group using mesoporous silica nanoparticles (MSNs) loaded with the redox probe methylene blue and capped with an avidin–imminobiotin (Av–ImB) stimulus–responsive gate-like ensemble (Figure 7b top) as the signal amplification element in connection with an aptasensor for CEA constructed by attaching a biotin and thiol-functionalized anti-CEA DNA hairpin aptamer on AuNPs–SPCEs [50].…”

Section: Biosensing and Delivery Of Nucleic Acids: Roles Opportunmentioning

confidence: 99%

“…Schematic display of the protocols used to functionalize the nanoparticles (top) and their use for electrochemical CEA aptasensing (down). Reprinted and adapted from [49] ( a ) and [50] ( b ) with permission. Copyrights Elsevier, 2019 ( a , b ).…”

Section: Figurementioning

confidence: 99%

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Biosensing and Delivery of Nucleic Acids Involving Selected Well-Known and Rising Star Functional Nanomaterials

et al. 2019

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In the last fifteen years, the nucleic acid biosensors and delivery area has seen a breakthrough due to the interrelation between the recognition of nucleic acid’s high specificity, the great sensitivity of electrochemical and optical transduction and the unprecedented opportunities imparted by nanotechnology. Advances in this area have demonstrated that the assembly of nanoscaled materials allows the performance enhancement, particularly in terms of sensitivity and response time, of functional nucleic acids’ biosensing and delivery to a level suitable for the construction of point-of-care diagnostic tools. Consequently, this has propelled detection methods using nanomaterials to the vanguard of the biosensing and delivery research fields. This review overviews the striking advancement in functional nanomaterials’ assisted biosensing and delivery of nucleic acids. We highlight the advantages demonstrated by selected well-known and rising star functional nanomaterials (metallic, magnetic and Janus nanomaterials) focusing on the literature produced in the past five years.

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Simple “Signal‐Off” Electrochemical Aptasensor Based on Aptamer‐Cu₃(PO₄)₂ Hybrid Nanoflowers/Graphene Oxide for Carcinoembryonic Antigen Detection

Zou

Guo

et al. 2020

ChemElectroChem

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A signal-off aptasensor was described based on well-designed aptamer-Cu 3 (PO 4 ) 2 hybrid nanoflowers&graphene oxide composites (aptamer-Cu 3 (PO 4 ) 2 HNF&GO) as an effective sensing platform and molybdophosphate precipitation as the signaling element. The aptamer, as the biorecognition unit grafted on aptamer-Cu 3 (PO 4 ) 2 HNF&GO by the specificity of streptavidin and biotin, captured the carcinoembryonic antigen (CEA) through specific interaction. The molybdophosphate precipitation were formed by the reaction of the aptamer-Cu 3 (PO 4 ) 2 HNF&GO with molybdate (MoO 4 2 À ). However, the CEAaptamer bioconjugate layer films not only hinder electron transfer on the sensing interface, but also impede the formation of molybdophosphate precipitation, resulting that the current signal significantly decreased. So, a simple aptasensor for CEA was constructed with high sensitivity and signal-off model. Under optimal experimental conditions using square wave voltammetry (SWV), the response peak currents decreases linearly with the logarithm of CEA concentrations in the range from 10 fg mL À 1 to 500 ng mL À 1 . Also, the feasibility of its applications has also been demonstrated in the analysis of clinical serum samples.

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Amperometric aptasensor for carcinoembryonic antigen based on the use of bifunctionalized Janus nanoparticles as biorecognition-signaling element

Cited by 51 publications

References 43 publications

Electrochemical Aptasensors for Biological and Chemical Analyte Detection

Electrochemical Aptasensors for Biological and Chemical Analyte Detection

Biosensing and Delivery of Nucleic Acids Involving Selected Well-Known and Rising Star Functional Nanomaterials

Simple “Signal‐Off” Electrochemical Aptasensor Based on Aptamer‐Cu₃(PO₄)₂ Hybrid Nanoflowers/Graphene Oxide for Carcinoembryonic Antigen Detection

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Amperometric aptasensor for carcinoembryonic antigen based on the use of bifunctionalized Janus nanoparticles as biorecognition-signaling element

Cited by 51 publications

References 43 publications

Electrochemical Aptasensors for Biological and Chemical Analyte Detection

Electrochemical Aptasensors for Biological and Chemical Analyte Detection

Biosensing and Delivery of Nucleic Acids Involving Selected Well-Known and Rising Star Functional Nanomaterials

Simple “Signal‐Off” Electrochemical Aptasensor Based on Aptamer‐Cu3(PO4)2 Hybrid Nanoflowers/Graphene Oxide for Carcinoembryonic Antigen Detection

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Resources

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Simple “Signal‐Off” Electrochemical Aptasensor Based on Aptamer‐Cu₃(PO₄)₂ Hybrid Nanoflowers/Graphene Oxide for Carcinoembryonic Antigen Detection