<i>In Vitro</i> Propagation of Grapevine Cultivars with Potential for South of Brazil

Abstract

The micropropation is an important biotechnological tool for obtaining and maintaining mother vine plants with high quality plant health. The objective was to evaluate the establishment and multiplication in vitro and ex vitro acclimatization of grape genotypes with potential for Southern Brazil. Vine nodal segments were cultured in five culture medium formulations without adding growth regulators. It was evaluated the number of leaves and roots, length of roots and shoots, replication rate, relative chlorophyll index, percentage of regenerated and rooted plants, dry biomass of shoot, root and total plants grown in vitro and after acclimatization. In vitro propagation of IAC 571-6 rootstock and cv. Poloskei Muskotaly through nodal segments provided high rates of regeneration and rooting. High survival rates were obtained in the acclimatization of IAC 571-6 and Pölöskei Muskotaly. Considering all the variables, the culture medium Roubelakis showed the best growth rates and development for shoots and roots, and in vitro multiplication rate for IAC 571-6 and Poloskei Muskotaly grape varieties.

FREE CULTURE MEDIA OF GROWTH REGULATORS ON MICROPROPAGATION OF GRAPEVINE (Vitis labrusca L.) ‘BORDÔ’ CULTIVAR THROUGH NODAL SEGMENTS

“…9 However, inadequate concentrations of cytokinin can promote the formation of anomalous shoots and multiplication in the form callus, 8 which is undesirable for vegetative propagation protocols, especially the risk of induced somaclonal mutation. Recent study described by Bettoni et al, 3 with Vitis, demonstrate that free salt formulations of growth regulators provided high in vitro multiplication rates and grapevines had root formation in various free culture media growth regulators and or specific phase for rooting. Thus, the aim of this study was to evaluate the establishment and multiplication in vitro in different culture media without adding growth regulators.…”

“…However, the availability of free of pathogens materials is a limiting factor for the sustainability of agricultural production and the permanence in the activity. 3 In this sense, micropropagation techniques are important tools for obtaining and maintaining stock plants of good quality phytosanitary. 4 The process enables the mass multiplication of plants and the formation of free virus matrices; besides preservation materials of interest in an aseptic environment.…”

“…8 Thus, the choice of a suitable salt formulation has a direct relationship with the successful development of in vitro cultures. 3,8 Published protocol with Vitis suggest the addition of plant growth regulators in the culture media, particularly from the group of the cytokinins, to promote multibrotation. 9 However, inadequate concentrations of cytokinin can promote the formation of anomalous shoots and multiplication in the form callus, 8 which is undesirable for vegetative propagation protocols, especially the risk of induced somaclonal mutation.…”

Effect of growth regulators on indirect organogenesis of two grapevines (Vitis vinifera L.) cultivars

“…The organogenesis is a biotechnological tool used for obtaining mass production of mother plant with high quality of health (Bettoni et al, 2015). The explants can be grown into whole plant or produce callus.…”

Cryotherapy: A New Technique to Obtain Grapevine Plants Free of Viruses

“…Although there is a favorable commercial scenario, area planted with grapevine and the harvest in the south of Brazil have been declining, it is observed that new vineyards face low productivity and poor quality of grapes due to several complex issues. In relation to plant health, one of the most important obstacles is the infection of vines with viruses, resulting in vine decline and death (BETTONI et al, 2015;GARRIDO et al, 2004).…”