&amp;lt;i&amp;gt;In Vitro&amp;lt;/i&amp;gt; Propagation and Cytological Analysis of &amp;lt;i&amp;gt;Boerhaavia diffusa &amp;lt;/i&amp;gt;L—An Important Medicinal Plant Species of Bangladesh

Majumder, Sweety; Biswas, Animesh; Rahman, Mohammad Mahbubur

doi:10.4236/ajps.2021.129095

Cited by 3 publications

(1 citation statement)

References 15 publications

(14 reference statements)

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“…[ 38 ]. Meanwhile, in Boerhaavia diffusa L., 2.0 mg·L −1 BAP produced the maximum mean number of shoot buds (6.65 per explant) [ 39 ]. Taken together, these reports imply that the variation in the optimum BAP concentration for shoot formation is linked to the type of explant and plant species [ 13 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

et al. 2023

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Background Codonopsis pilosula (Franch.) Nannf. is a medicinal plant traditionally used in China, Korea, and Japan to treat many diseases including poor gastrointestinal function, low immunity, gastric ulcers, and chronic gastritis. The increasing therapeutic and preventive use of C. pilosula has subsequently led to depletion of the natural populations of this species thus necessitating propagation of this important medicinal plant. Here, we developed an efficient and effective in vitro propagation protocol for C. pilosula using apical shoot segments. We tested various plant tissue culture media for the growth of C. pilosula and evaluated the effects of plant growth regulators on the shoot proliferation and rooting of regenerated C. pilosula plants. Furthermore, the tissues (roots and shoots) of maternal and in vitro-regenerated C. pilosula plants were subjected to Fourier-transform near-infrared (FT-NIR) spectrometry, Gas chromatography-mass spectrometry (GC–MS), and their total flavonoids, phenolics, and antioxidant capacity were determined and compared. Results Full-strength Murashige and Skoog (MS) medium augmented with vitamins and benzylaminopurine (1.5 mg·L−1) regenerated the highest shoot number (12 ± 0.46) per explant. MS medium augmented with indole-3-acetic acid (1.0 mg·L−1) produced the highest root number (9 ± 0.89) and maximum root length (20.88 ± 1.48 mm) from regenerated C. pilosula shoots. The survival rate of in vitro-regenerated C. pilosula plants was 94.00% after acclimatization. The maternal and in vitro-regenerated C. pilosula plant tissues showed similar FT-NIR spectra, total phenolics, total flavonoids, phytochemical composition, and antioxidant activity. Randomly amplified polymorphic DNA (RAPD) test confirmed the genetic fidelity of regenerated C. pilosula plants. Conclusions The proposed in vitro propagation protocol may be useful for the rapid mass multiplication and production of high quality C. pilosula as well as for germplasm preservation to ensure sustainable supply amidst the ever-increasing demand.

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Section: Discussionmentioning

confidence: 99%

In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

et al. 2023

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An assessment for in vitro propagation and genetic stability of Phoebe goalparensis Hutchinson, an endemic valuable timber tree of North East India

Mudoi

Gogoi

Borah

et al. 2023

Journal of Genetic Engineering and Biotechnology

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Background Phoebe goalparensis is an endemic forest species of North East India that belongs to Lauraceae family. P. goalparensis is used as timbers yielding plants for commercial importance in the local furniture markets of North East India. A rapid in vitro micropropagation protocol was established by using apical and axillary shoot tips on Murashige and Skoog medium with varied concentrations of plant growth regulators. Results In this study, 5.0 mg/l BAP augmented medium was chosen as the best for shoot multiplication of the plant. However, IBA (2.0 mg/l) was the most responsive for root induction. Moreover, 70% of root induction was recorded during rooting experiment and 80–85% survivability was observed during the acclimatization of this species. Clonal fidelity of P. goalparensis was determined with ISSR marker and it was observed that in vitro raised plantlets were polymonomorphic. Conclusion Hence, an efficient protocol with high proliferation and rooting was established for P. Goalparensis that could aid in massive propagation in future.

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Shoot Induction, Multiplication, Rooting and Acclimatization of Black Turmeric (Curcuma caesia Roxb.): An Important and Endangered Curcuma Species

et al. 2022

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Curcuma caesia Roxb., commonly known as Kali Haldi or black turmeric, is one of the important species in the genus Curcuma. This species has been classified as one of the endangered Curcuma species due to the drastic decrement of this plant in its natural habitat. C. caesia has been overharvested for various purposes, including bioactive compound extraction to fulfill the pharmaceutical industry demand. Hence, this study was conducted to establish a protocol for the propagation of C. caesia via plant tissue culture techniques. In the shoot induction stage, three basal medium formulations, including Murashige and Skoog (MS medium), the combination of Murashige and Skoog macronutrients and B5 micronutrients (MSB5 medium) and woody plant medium (WPM medium) supplemented with 15 μM of 6-benzylaminopurine (BAP), were used. The results found that the MSB5 medium was the most suitable basal medium formulation for shoot induction of C. caesia. In the subsequent experiment, different types of cytokinin, including BAP, kinetin and 2-iP at concentrations of 5, 10, 15 and 20 μM, were fortified in the MSB5 medium for shoot multiplication. The shoot multiplication was further enhanced by supplementing the MSB5 medium with indole-3-butyric acid (IBA) or 1-napthaleneacetic acid (NAA) at the concentrations of 2, 4, 6 and 8 μM. The results showed that a combination of 15 μM of BAP and 6 μM of IBA significantly increased the shoot multiplication with 100% shoot induction, 3.53 shoots/explant, 10.81 cm of shoot length, 9.57 leaves, 0.486 g of leaves fresh weight and 0.039 g of leaves dry weight. After the multiplication, the rooting stage was carried out by altering the basal medium strength into half and full strength and supplementing with 2.5, 5, 7.5 and 10 μM of indole-3-acetic acid (IAA). The full strength of MSB5 medium supplemented with 5 μM of IAA exhibited the highest number of roots and length of roots, with 6.13 roots and 5.37 cm, respectively. After the rooting stage, the plantlets were successfully acclimatized in the potting medium with the combination of cocopeat and peatmoss, and the ratio of 1:1 was found to produce the highest survival rate with 77.78%. In conclusion, the protocol established in this study could be useful for large-scale raw material production, either for conservation or bioactive compound extraction.

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<i>In Vitro</i> Propagation and Cytological Analysis of <i>Boerhaavia diffusa </i>L—An Important Medicinal Plant Species of Bangladesh

Cited by 3 publications

References 15 publications

In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

An assessment for in vitro propagation and genetic stability of Phoebe goalparensis Hutchinson, an endemic valuable timber tree of North East India

Shoot Induction, Multiplication, Rooting and Acclimatization of Black Turmeric (Curcuma caesia Roxb.): An Important and Endangered Curcuma Species

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