Shoot Induction, Multiplication, Rooting and Acclimatization of Black Turmeric (Curcuma caesia Roxb.): An Important and Endangered Curcuma Species

Haida, Zainol; Sinniah, Uma Rani; Nakasha, Jaafar Juju; Hakiman, Mansor

doi:10.3390/horticulturae8080740

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…Similar effects of mT on the height of micro-plantlets were reported by other researchers who studied various horticultural species (Moyo et al, 2018;Kucharska et al, 2020;Nowakowska et al, 2020). Other studies found that 2-ip might have different effects on the length of the microshoot, but for some tree crops, 2-ip can positively affect shoot growth compared to 6-BAP (Debnath;Kenneth, 2001;Faisal et al, 2006;Singh, 2020;Wulandari et al, 2021;Haida et al, 2022). The studied plant growth regulators could be arranged according to their effect on the ability to promote shoot formation in the following order: mT > 6-BAP > 2-ip.…”

Section: Shoot Multiplicationsupporting

confidence: 76%

Plant growth regulators on the micropropagtion of Actinidia cultivars

Krakhmaleva,

Molkanova,

Orlova

et al. 2023

Ciênc. agrotec.

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Actinidia Lindl., commonly known as kiwifruit, is a valuable berry crop. The area of commercial kiwifruit plantations is increasing; the global production of kiwifruit is about 0.62% of the total production of major fruit crops. The use of biotechnological methods, which can significantly accelerate the propagation of quality planting materials, is considered to be relevant for the propagation of this crop. In this study, we optimized the culture medium composition at the micropropagation stage for the effective cultivation of promising cultivars of A. arguta, A. kolomikta, and A. polygama. We investigated the features of Actinidia morphogenesis depending on the genotype, the concentration of 6-Benzylaminopurine (0.5, 0.8, and 1.0 mg L-1), and plant growth regulators (6-Benzylaminopurine, meta-topolin, and 2-isopentenyladenine at a concentration of 0.5 mg L-1) in the media Quoirin and Lepoivre. Actinidia arguta (multiplication rate of 8.0) and A. polygama (6.8) developed faster at the micropropagation stage compared to A. kolomikta (4.9). The studied Actinidia representatives were cultured most effectively on Quoirin and Lepoivre media supplemented with 0.5 mg L-1 meta-topolin, compared to the media containing 0.5 mg L-1 6-Benzylaminopurine and 0.5 mg L-1 2-isopentenyladenine. The use of meta-topolin in the medium contributed to the increase in various morphometric traits, such as the height of microshoots (up to 28% depending on the species), their number (up to 52%), and their multiplication rate (up to 42%). We also recorded a high morphogenic capacity of the investigated species.

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Section: Shoot Multiplicationsupporting

confidence: 76%

Plant growth regulators on the micropropagtion of Actinidia cultivars

Krakhmaleva,

Molkanova,

Orlova

et al. 2023

Ciênc. agrotec.

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“…Plant tissue culture is used for large-scale plant reproduction, virus elimination, secondary metabolite synthesis, and in vitro cloning (Ebrahimzadegan and Marou 2022; Haida et al 2022). Many studies have shown that the healing rate of Hemerocallis ower organs in tissue culture is higher than that of other parts, and the ower stem, scape, pedicel and petals are commonly used as explants (Wang 2013).…”

Section: Discussionmentioning

confidence: 99%

Establishment of efficient callus genetic transformation system for Hemerocallis fulva ‘Kanai’

Shi

Pan

et al. 2022

Preprint

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Hemerocallis is regarded as a model plant for future breeding because of its high ornamental value and strong resistance. However, there are few reports on regeneration and genetic transformation of this plant. In this study, mature seeds of Hemerocallis fulva ‘Kanai’ were used as explants for regeneration and Agrobacterium infection. The results showed that the germination rate of explants with inner and outer seed coats peeled was 98% and the contamination rate was only 11%. The callus induction efficiency of MS medium supplemented with 3.5 mg∙L− 1 6-Benzylaminopurine (6-BA) and 0.1 mg∙L− 1 1-naphthylcetic acid (NAA) was 95.2%. Based on this, transformation was successfully achieved using the following protocol: callus were soaked in Agrobacterium tumefaciens EHA105 (OD600 = 0.6) containing pCambia1300-35S-FT and pCambia1300-35S-GUS plasmid for 15 min. After 3 days co-culture with 100 uM Acetosyringone (AS) in MS medium, it was transferred to MS medium containing 300 mg∙mL− 1 Timentin for 5 days. The Transgenic plants were obtained by hygromycin (9 mg∙mL− 1) screening. The presence of transgenic plants was confirmed by histochemical GUS detection and PCR (Polymerase chain reaction). Overall, the establishment of this efficient regeneration and genetic transformation will contribute to the functional gene research and genetic improvement of Hemerocallis fulva ‘Kanai’.

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“…For instance, Curcuma caesia Roxb. was successfully acclimatized in the potting medium by combining cocopeat and peat moss, producing the highest survival rate of 77.78% [10]. Success in acclimatizing tissue-cultured plants depends on factors like plantlet size and root condition, significantly affecting nutrient absorption [11].…”

Section: Acclimatization In Greenhousementioning

confidence: 99%

Optimizing Growth Conditions for Green Grass Jelly (Cyclea barbata Miers) Derived in Vitro Culture: Greenhouse Acclimatization and Field Growth

Heryanto,

Fatimah Syahid

2023

E3S Web of Conf.

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Cyclea barbata Miers, a species within the Menispermaceae family, holds significance in medicinal and beverage applications. In vitro propagation is a common practice for conserving this plant; however, a comprehensive investigation of its growth characteristics within controlled greenhouse and natural field environments is essential. This study aims to determine the optimal planting media for acclimatization and the subsequent growth of Cyclea barbata Miers propagated from in vitro cultures. The experimental design encompasses two phases: 1) greenhouse acclimatization and 2) field growth. Various planting media, including combinations of sterile soil, manure, and husk charcoal, were rigorously evaluated during the acclimatization phase. Following the acclimatization period, the plants were transferred to the field. Multiple parameters were assessed: plant growth percentage, branch and node counts, leaf dimensions (length and width), and leaf wet and dry weights. Our findings reveal that a planting medium comprising sterile soil, manure, and husk charcoal provided the best performance growth outcomes during acclimatization. Furthermore, we observed that plant age exerts a discernible influence on the growth dynamics of Cyclea barbata Miers.

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Shoot Induction, Multiplication, Rooting and Acclimatization of Black Turmeric (Curcuma caesia Roxb.): An Important and Endangered Curcuma Species

Cited by 7 publications

References 33 publications

Plant growth regulators on the micropropagtion of Actinidia cultivars

Plant growth regulators on the micropropagtion of Actinidia cultivars

Establishment of efficient callus genetic transformation system for Hemerocallis fulva ‘Kanai’

Optimizing Growth Conditions for Green Grass Jelly (Cyclea barbata Miers) Derived in Vitro Culture: Greenhouse Acclimatization and Field Growth

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