&amp;lt;i&amp;gt;In Situ&amp;lt;/i&amp;gt; Hydroxyapatite Synthesis: Influence of Collagen on Its Structural and Morphological Characteristic

Mendes, Luís Claudio; Ribeiro, Geysy L.; Marques, Raphaella C.

doi:10.4236/msa.2012.38083

Cited by 24 publications

(16 citation statements)

References 32 publications

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“…These infrared characteristic peaks in Fig. 3a are consistent with the spectra of type I collagen of fish reported in the literatures, indicating that the triple helix structure was retained [41,42]. Fig.…”

Section: Ftir Characterizationsupporting

confidence: 89%

Preparation and characterization of novel poly (vinyl alcohol)/collagen double-network hydrogels

Wang

et al. 2018

International Journal of Biological Macromolecules

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Hydrogels prepared by conventional methods show poor performance in many aspects. Many double network (DN) hydrogels prepared by crosslinking agents have disadvantages such as toxicity. In this work, we prepared novel DN hydrogels with fish-originated collagen (Col) and poly (vinyl alcohol) (PVA), in which self-assembly of collagen and self-crosslinking of PVA were achieved. Infrared spectra indicated the existence of double network with chemical interactions between Col and PVA. X-ray diffraction (XRD) patterns showed that characteristic peak of freeze-thaw PVA in DN hydrogel was retained. Scanning electron microscope (SEM) images before and after degradation and swelling property tests indicated that the morphology of the hydrogel was a compact meshwork, which is consistent with the high water-retention rate. The degradation rate of the DN hydrogels was controlled by the ratio of Col and PVA. Compared with a pure collagen hydrogels, the stress of DN hydrogels was greatly enhanced from 6 to 33 kPa at a strain of 40%. This study indicates that DN hydrogels prepared by Col and PVA are an ideal biomaterial for tissue engineering.

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Section: Ftir Characterizationsupporting

confidence: 89%

Preparation and characterization of novel poly (vinyl alcohol)/collagen double-network hydrogels

Wang

et al. 2018

International Journal of Biological Macromolecules

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“…Furthermore, precipitation in simulated body fluid has such a low yield and long reaction time that it would not be practically feasible for large scale production. Similarly there are many reports on collagen mediated mineralization like Mendes et al where a temperature of 80˚C for approximately 40 min was required for the synthesis [39]. We are not the first to report biomimetic synthesis of HA.…”

Section: Introductionmentioning

confidence: 49%

Biomimetic matrix mediated room temperature synthesis and characterization of nano-hydroxyapatite towards targeted drug delivery

2016

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The nucleation and growth of hydroxyapatite is closely associated with the extracellular matrix environment. Bovine Serum Albumin, Collagen and poly vinyl alcohol were used to mimic the extracellular matrix. An attempt to understand the role of these matrices on the synthesis and function of Hydroxyapatite has been made. XRD, FT-IR, XPS, TG-DTA, SEM and TEM confirmed the formation of hydroxyapatite synthesized by the biomimetic route. Further the role of organic matrix in controlling the nucleation and growth of hydroxyapatite particles in the nano level is understood by the in-depth analysis of the XPS spectra. The in vitro release of the anti-cancer drug methotrexate, in aqueous solution was studied and the in vitro release profile was assayed by elution in phosphate buffered saline with pH 7.4 and pH 5 at 37 º C. The percentage of loading and release profiles of drug were evaluated. The results show that the use of matrix increased the drug release efficiency from 44.5% to 66% at pH 7.4 and 78% to 98.92% at pH 5. These results suggest that the synthesized hydroxyapatite can be used as a pH responsive vehicle for delivering drugs. Further the release profile was predicted by Higuchi and Peppas model. The results suggested that the release mechanism was governed by the Fickian diffusion for initial 8 hrs followed by anomalous transport for longer time. The cytocompatibility of the materials was evaluated by in vitro cytotoxicity test. Both MTT and live/dead assay observations indicated that the material had no adverse impact on the cell proliferation. The results imply these composites to be bioactive having a good cytocompatibility. Although all the matrices showed good results, the one with poly vinyl alcohol exhibited higher biocompatibility and drug release efficiency. A plausible explanation was proposed for the enhanced drug delivery efficiency of these materials.

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“…Nanoscience can be applied in different fields such as pharmaceutical, agricultural, medical and industrial fields. Gelatin is a soluble polypeptide protein derived from insoluble collagen, which is the most abundant protein in animal skin and bone [2, 3]. Gelatin is classified according to the method of separation: type A gelatin is derived from acid‐cured tissues and type B is derived from lime‐cured tissues.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles using bovine skin gelatin and its antibacterial effect on clinical bacterial isolates

Salaheldin¹,

Almalki

Osman

2017

IET nanobiotechnol.

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Nanoparticles are being increasingly used in day-to-day life. Therefore, concerns have been raised regarding their interactions with the surrounding environment. This study focused on a simple green method for synthesizing silver nanoparticles (Ag-NPs) in an autoclave at 15 psi (103 kPa) and 121°C. An aqueous solution of AgNO as a precursor of Ag-NPs and gelatin (type B) reducing and/or stabilizing (capping) agent were used. The effect of various AgNO concentrations of certain gelatin concentration and various gelatin concentrations at constant AgNO concentration, and autoclaving time, was studied. UV-Vis spectra ascribed that the presence of localized surface plasmon resonance (SPR) of the synthesized Ag-NPs. TEM images and the selected area of electron diffraction confirmed, the formation of Ag-NPs with a diameter of approximately 5 ±0.35 nm. Furthermore, FT-IR revealed that a gelatin polymer matrix stabilized the synthesized Ag-NPs. The Well diffusion assay was used to test the effect of Ag-NPs on six clinical bacterial isolates, where Gram positive bacteria were more susceptible to Ag-NPs than Gram negative bacteria. Therefore, Ag-NPs capped by gelatin have remarkable potential effect as an antibacterial agent, and they not only have various medical applications but can also be used in biological, pharmaceutical and industrial fields.

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<i>In Situ</i> Hydroxyapatite Synthesis: Influence of Collagen on Its Structural and Morphological Characteristic

Cited by 24 publications

References 32 publications

Preparation and characterization of novel poly (vinyl alcohol)/collagen double-network hydrogels

Preparation and characterization of novel poly (vinyl alcohol)/collagen double-network hydrogels

Biomimetic matrix mediated room temperature synthesis and characterization of nano-hydroxyapatite towards targeted drug delivery

Green synthesis of silver nanoparticles using bovine skin gelatin and its antibacterial effect on clinical bacterial isolates

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