Green synthesis of silver nanoparticles using bovine skin gelatin and its antibacterial effect on clinical bacterial isolates

Abstract

Nanoparticles are being increasingly used in day-to-day life. Therefore, concerns have been raised regarding their interactions with the surrounding environment. This study focused on a simple green method for synthesizing silver nanoparticles (Ag-NPs) in an autoclave at 15 psi (103 kPa) and 121°C. An aqueous solution of AgNO as a precursor of Ag-NPs and gelatin (type B) reducing and/or stabilizing (capping) agent were used. The effect of various AgNO concentrations of certain gelatin concentration and various gelatin concentrations at constant AgNO concentration, and autoclaving time, was studied. UV-Vis spectra ascribed that the presence of localized surface plasmon resonance (SPR) of the synthesized Ag-NPs. TEM images and the selected area of electron diffraction confirmed, the formation of Ag-NPs with a diameter of approximately 5 ±0.35 nm. Furthermore, FT-IR revealed that a gelatin polymer matrix stabilized the synthesized Ag-NPs. The Well diffusion assay was used to test the effect of Ag-NPs on six clinical bacterial isolates, where Gram positive bacteria were more susceptible to Ag-NPs than Gram negative bacteria. Therefore, Ag-NPs capped by gelatin have remarkable potential effect as an antibacterial agent, and they not only have various medical applications but can also be used in biological, pharmaceutical and industrial fields.

Copper/copper oxide nanoparticles synthesis usingStachys lavandulifoliaand its antibacterial activity

“…Some of the important applications of these materials include fabrication of sensors and biosensors [20][21][22], optical and electronic devices [23], energy storage devices [24], and antibacterial materials [25]. Copper and its oxides are relatively non-toxic to mammals [26], low sensitivity for human tissue [27], and antimicrobial activity [25,28].…”

Porcine skin gelatin–silver nanocomposites: synthesis, characterisation, cell cytotoxicity, and antibacterial properties

“…Previously, we investigated the synthesis of AgNPs with bovine skin gelatin (type B), which showed a remarkable effect as antibacterial agent [18]. Whereas, this study demonstrates the antibacterial properties of gram-positive and gram-negative pathogenic bacteria, cell cytotoxicity, and antioxidant activity of AgNPs synthesised using PSG (type A).…”

“…In general, reducing agents are used to reduce the metal ions to metallic NPs. Some of these reducing agents are toxic chemicals such as sodium borohydride [8,9], ethylene glycol [10], dimethyl formamide [11,12], and hydrazine hydrate [13], whereas some are non-toxic in nature, such as glucose [14][15][16], chitosan [4], starch [17], and gelatin [18].…”

Overexpression and Cytocidal Activity of Parasporin-1 from Bacillus thuringiensis against Human Cancer Cell Lines

“…It may be hypothesized that, by an analogy to insecticidal cry proteins, PS3Aa1 may act as a pore-forming toxin on the plasma membrane of cancer cells. This is supported, in part, by the fact that PS3Aa1 increases plasma membrane permeability of the target cells [44][45][46]. It is also well known that the insecticidal Cry1A proteins induce cell death through pore formation in plasma membrane after initial binding to the GPI-anchored receptors, aminopeptidases and cadherin-like proteins in the midgut epithelial cells of the susceptible lepidopteran insects [47].…”