Amniotic Membrane Wrapping: An Alternative Method to the Splenorrhaphy in the Injured Spleen

Abstract

Human amnion, which has been used experimentally and clinically as an ideal bioprosthesis, was used in the present study to wrap the experimentally injured spleens and the technique was compared to splenorrhaphy. Standard splenic injuries were made on 30 male albino rats. 15 of the spleens were repaired by splenorrhaphy and the other 15 were wrapped with sterile human amniotic membrane. Isotope uptakes of the spleens calculated at the 30th postoperative day were similar in both groups (p less than 0.05). But the histopathological examination revealed a better healing in the amnion group with better preservation of the splenic architecture. It was concluded that in heavily injured spleens, amnion wrapping may be an effective alternative to splenorrhaphy.

Transplantation of Human Umbilical Cord Blood or Amniotic Epithelial Stem Cells Alleviates Mechanical Allodynia after Spinal Cord Injury in Rats

“…Although there are some basic differences between hUCB-MSCs and hAESCs, including their cellular morphology and intrinsic characteristics, they both have the capacity for self-renewal and differentiation into a variety of diverse cell types (37,58). AESCs express surface markers normally present on embryonic stem and germ cells, and several studies have examined the use of these cells for treatment of a variety of conditions including burns (10,11), defects in the abdominal wall and ruptured spleens (15), and oral wounds (31). Additionally, there are several reports that suggest the use of hAESCs for a number of therapeutic applications (36,62).…”

The Use of Dehydrated Amniotic Membrane Allograft for the Augmentation of Dural Repair in Craniotomies

“…Dehydrated amniotic membrane (DAM) has been shown to inhibit fibrosis and scar formation, prevent an immune response, promote epithelization, and have antibacterial and anti-inflammatory properties [ 8 ]; thus, it may be an ideal dural substitute. DAM has been successfully utilized in wound closure in multiple surgical subspecialties, including plastic surgery [ 9 - 10 ], general surgery [ 11 ], otolaryngology [ 12 ], maxillofacial surgery [ 13 ], ophthalmology [ 14 ], and spinal surgery [ 15 - 16 ]. However, its use is not described in the cranial neurosurgery literature.…”

Autologous amnion graft for repair of myelomeningocele: technical note and clinical implication

“…The usefulness of human amniotic membrane, composed of amnion and chorion, has been experimentally and clinically reported mainly in plastic surgical field including dressing burn wounds, 1;2 patching the defects of abdominal wall, ruptured spleen, 3 repair of tympanic membranes, 4 oral wounds, 5 and others. 6 Recently, interest has been ophthalmologically renewed in the use of this tissue for several types of corneal defect.…”