Amniotic fluid l-ergothioneine concentrations in pregnant sheep after natural mating and transfer of vitrified/thawed in-vitro produced embryos

Sotgia, Salvatore; Zinellu, Angelo; Arru, Dionigia; Nieddu, Stefano; Strina, Alessandro; Ariu, Federica; Pintus, Gianfranco; Carru, Ciriaco; Bogliolo, Luisa; Ledda, Sergio

doi:10.1016/j.rvsc.2015.09.003

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…Leaving aside early efforts such as the colorimetric methods of Hunter (365) , of Melville and colleagues (76,85,366) and of Carlsson et al (367) , a variety of analytical methods have been proposed (4) , mostly involving capillary electrophoresis (368,369) or chromatography (368,(370)(371)(372) coupled to absorbance (373,374) , fluorescence detection (375)(376)(377)(378) , electrochemical detection (379) or MS (72,127,256,368,378,(380)(381)(382) . A useful feature is that ERG is unusually stable, in that anhydrous ERG decomposes only at 275-276°C (76) , allowing its isolation at temperatures close to that of boiling water (72) .…”

Section: Analyticsmentioning

confidence: 99%

The biology of ergothioneine, an antioxidant nutraceutical

et al. 2020

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Ergothioneine (ERG) is an unusual thio-histidine betaine amino acid that has potent antioxidant activities. It is synthesised by a variety of microbes, especially fungi (including in mushroom fruiting bodies) and actinobacteria, but is not synthesised by plants and animals who acquire it via the soil and their diet, respectively. Animals have evolved a highly selective transporter for it, known as solute carrier family 22, member 4 (SLC22A4) in humans, signifying its importance, and ERG may even have the status of a vitamin. ERG accumulates differentially in various tissues, according to their expression of SLC22A4, favouring those such as erythrocytes that may be subject to oxidative stress. Mushroom or ERG consumption seems to provide significant prevention against oxidative stress in a large variety of systems. ERG seems to have strong cytoprotective status, and its concentration is lowered in a number of chronic inflammatory diseases. It has been passed as safe by regulatory agencies, and may have value as a nutraceutical and antioxidant more generally.

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Section: Analyticsmentioning

confidence: 99%

The biology of ergothioneine, an antioxidant nutraceutical

et al. 2020

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“…This implies that ET can cross the placenta into the baby (presumably via OCTN1, which is known to be present in placenta ) and/or that ET is absorbed from breast milk through OCTN1 in the intestines of the baby. Additionally, ET has been detected in amniotic fluid, in sheep . Studies revealed that the OCTN1 mRNA expression levels in cultured human mammary epithelial cells are elevated more than sixfold during lactation, relative to nonlactating mammary epithelial cells .…”

Section: Ergothioneine and The Mother–baby Axismentioning

confidence: 99%

Ergothioneine – a diet‐derived antioxidant with therapeutic potential

2018

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Ergothioneine is a thiol/thione molecule synthesised only by some fungi and bacteria. Nonetheless, it is avidly taken up from the diet by humans and other animals through a transporter, OCTN1, and accumulates to high levels in certain tissues. Ergothioneine is not rapidly metabolised, or excreted in urine and is present in many, if not all, human tissues and body fluids. Ergothioneine has powerful antioxidant and cytoprotective properties in vitro and there is evidence that the body may concentrate it at sites of tissue injury by raising OCTN1 levels. Decreased blood and/or plasma levels of ergothioneine have been observed in some diseases, suggesting that a deficiency could be relevant to the disease onset or progression. This brief Review explores the possible roles of ergothioneine in human health and disease.

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“…Through a specific organic protein transporter (ETT), ERT accumulates in mitochondria, cells, and tissues normally exposed to oxidative stress and involved in the inflammatory response process [1,3]. Unexpected high levels of ERT in red blood cells has been observed in some autoimmune disorders [5,6], such as rheumatoid arthritis and Crohn’s disease, in pre-eclamptic women [7] and, recently, in amniotic fluid of pregnant sheep after transfer of vitrified/thawed in vitro produced embryos [8]. Similar to glutathione (GSH), several observations suggest that ERT might act as antioxidant and scavenger of oxidizing species [9].…”

Section: Introductionmentioning

confidence: 99%

Identification of the Main Intermediate Precursor of l-Ergothioneine Biosynthesis in Human Biological Specimens

et al. 2016

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A capillary electrophoresis coupled to tandem mass spectrometry (CE-MS/MS) has been used to make a qualitative determination of hercynine-the main precursor of L-ergothioneine biosynthesis-in some key human biological specimens, such as urine, whole blood, plasma, and saliva. From semiquantitative analysis results, the highest concentrations of hercynine were detected in saliva and whole blood, whereas much lower concentrations were measured in urine and plasma. Whole blood was the biological matrix with the highest concentration of L-ergothioneine followed by plasma, saliva, and urine. The antioxidant effects attributed to L-ergothioneine, along with its peculiar antioxidant mechanism, offer a possible explanation for the presence of the hercynine, as well as its concentration, in the considered biological matrices.

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Amniotic fluid l-ergothioneine concentrations in pregnant sheep after natural mating and transfer of vitrified/thawed in-vitro produced embryos

Cited by 5 publications

References 29 publications

The biology of ergothioneine, an antioxidant nutraceutical

The biology of ergothioneine, an antioxidant nutraceutical

Ergothioneine – a diet‐derived antioxidant with therapeutic potential

Identification of the Main Intermediate Precursor of l-Ergothioneine Biosynthesis in Human Biological Specimens

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