Identification of the Main Intermediate Precursor of l-Ergothioneine Biosynthesis in Human Biological Specimens

Sotgia, Salvatore; Mangoni, Arduino A.; Forteschi, Mauro; Murphy, Rhys B.; Elliot, David J.; Sotgiu, Elisabetta; Pintus, Gianfranco; Carru, Ciriaco; Zinellu, Angelo

doi:10.3390/molecules21101298

Cited by 12 publications

(10 citation statements)

References 29 publications

(52 reference statements)

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“…Hercynine and L‐ergothioneine two metabolites of the histidine have putative antioxidant effect. To assess this theory, they were quantified in urine, whole blood, plasma, and saliva of healthy human patients by CE‐ESI + ‐MS/MS and detected in the range 66–0.2 μM . As oxidative stress balance is crucial for chronic obstructive pulmonary disease and asthma patients, CE/UV was used for L‐ergothioneine and taurine determination .…”

Section: Metabolomics Biomedical Studiesmentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

et al. 2017

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Recent advances in amino acid analysis by capillary electromigration methods: June 2015-May 2017In the tenth edition of this article focused on recent advances in amino acid analysis using capillary electrophoresis, we describe the most important research articles published on this topic during the period from June 2015 to May 2017. This article follows the format of the previous articles published in Electrophoresis. The new developments in amino acid analysis with CE mainly describe improvements in CE associated with mass spectrometry. Focusing on applications, we mostly describe clinical works, although metabolomics studies are also very important. Finally, works focusing on amino acids in food and agricultural applications are also described.

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Section: Metabolomics Biomedical Studiesmentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

et al. 2017

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“…1d ) could be generated through the methylation of the sulfur. Hercynine and S-methyl ET have been detected in human blood and urine, with high correlation to ET concentrations in the body 13 , 41 .…”

Section: Introductionmentioning

confidence: 99%

Distribution and accumulation of dietary ergothioneine and its metabolites in mouse tissues

Tang

Cheah

Yew

et al. 2018

Sci Rep

118

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L-ergothioneine (ET) is a diet-derived amino acid that accumulates at high concentrations in animals and humans. Numerous studies have highlighted its antioxidant abilities in vitro, and possible cytoprotective capabilities in vivo. We investigated the uptake and distribution of ET in various organs by a highly sensitive and specific liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) technique, both before and after oral administration of pure ET (35 and 70 mg/kg/day for 1, 7, and 28 days) to male C57BL6J mice. ET primarily concentrates in the liver and whole blood, and also in spleen, kidney, lung, heart, intestines, eye, and brain tissues. Strong correlations were found between ET and its putative metabolites - hercynine, ET-sulfonate (ET-SO3H), and S-methyl ET. Hercynine accumulates in the brain after prolonged ET administration. This study demonstrates the uptake and distribution of ET and provides a foundation for future studies with ET to target oxidative damage in a range of tissues in human diseases.

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“…Therefore, the overall ERT measurement might not discriminate between the ERT regenerated following oxidation and the whole ERT. As proposed in our previous studies [31,40], ERY measurement, instead of ERT alone, may overcome this issue, as this oxidative metabolite of ERT does not undergo regeneration processes [21,22]. In our study, ERY concentrations in stallion seminal plasma (global median value of 7.50 (IQR 15.26) nmol/L) were much lower and exhibited significant variability when compared to ERT.…”

Section: Discussionmentioning

confidence: 49%

“…Although the lack of any correlation between ERT and sperm motility is of difficult interpretation, given the antioxidant role suggested by the data, the peculiar redox cycle of ERT may account for this apparent discrepancy as it involves a fast recovery of ERT that potentially could hide concentration fluctuations that are linked to its biological activity [31,40]. Therefore, the overall ERT measurement might not discriminate between the ERT regenerated following oxidation and the whole ERT.…”

Section: Discussionmentioning

confidence: 89%

Hercynine, Ergothioneine and Redox State in Stallion’s Seminal Plasma

et al. 2020

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The dependence of a stallion’s spermatozoa on oxidative phosphorylation for energy requirements results in an unconventional relationship between reactive oxygen species (ROS) production and fertility. In such a scenario, antioxidant activity must be finely controlled and not affect the essential functions of ROS. Some in vivo evidence suggests that the naturally occurring antioxidant ergothioneine (ERT) interferes with the critical roles of ROS/reactive nitrogen species (RNS) in pro-oxidant states but not in healthy tissues. The measurement of ERT in seminal plasma collected from 14 stallions (five Anglo-Arab, five Sella Italiano and four Thoroughbreds of which three are Arabian and one English) aged 16 ± 6 years (range 6–25 years) confirms that ERT is present at high concentrations in this biological fluid, between 16.80 and 971.48 µmol/L. Although the presence of high ERT concentrations in the seminal plasma of a stallion has long been known, its exact biological role is uncertain. This might be due to the peculiar antioxidant cycle of ERT, specifically its rapid recovery, which potentially masks concentration fluctuations and, therefore, the extent of its physiological effects. The measurement of the ERT precursor and redox metabolite hercynine (ERY) may overcome such issues, as ERY does not undergo regeneration processes. ERY was detectable and measurable in the seminal plasma of all stallions at a median concentration of 7.50 (IQR 15.26) nmol/L. The analysis of the association between the ERT and ERY, as well as with other established antioxidants such as glutathione and cysteine, suggests that ERT may play a major role in the antioxidant machinery of seminal plasma, and that ERY might serve as a new combined marker of oxidative stress and semen quality.

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Identification of the Main Intermediate Precursor of l-Ergothioneine Biosynthesis in Human Biological Specimens

Cited by 12 publications

References 29 publications

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

Distribution and accumulation of dietary ergothioneine and its metabolites in mouse tissues

Hercynine, Ergothioneine and Redox State in Stallion’s Seminal Plasma

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