Amino Acid Composition of Semen and the Secretions of the Male Reproductive Tract

Pd, Brown-Woodman; White, I. G.

doi:10.1071/bi9740415

Cited by 20 publications

(7 citation statements)

References 24 publications

(26 reference statements)

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“…This seems a real possibility as high concentrations of Glu were found in epididymal homogenates (Table 2). This is consistent with findings (Hopwood & Gassner, 1962;Brown-Woodman & White, 1974) which have shown that Glu, the predominant amino acid in seminal plasma, is derived mainly from epididymal secretions. We suggest that this Glu may originate from GSH by the hydrolytic activity of y-GT.…”

Section: Discussionsupporting

confidence: 93%

Glutathione, L‐glutamic acid and γ‐glutamyl transpeptidase in the bull reproductive tissues

Agrawal

Vanha‐Perttula

1988

Int J of Andrology

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The distribution of glutathione (GSH), L-glutamic acid (Glu) and gamma-glutamyl transpeptidase (gamma-GT) was studied in bull reproductive organs and fluids. Glutathione, the physiological substrate of gamma-GT, was localized specifically by a fluorescence method in the testis, epididymis and spermatozoa. Of the reproductive tissues, the testis, caput epididymis and ampulla had the highest levels of GSH, but it was also present in seminal fluid. Washed caput epididymal sperm had three times the GSH content of cauda epididymal or ejaculated sperm. In spermatozoa, GSH displayed maximal staining in the midpiece and tail regions. The highest levels of gamma-GT were encountered in the epididymis. The concentration of Glu was also high in the epididymis. Its formation may be due to the hydrolytic activity of gamma-GT, which, in addition, may have an important role in the transfer of Glu residues to reactive groups on the sperm surface.

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Section: Discussionsupporting

confidence: 93%

Glutathione, L‐glutamic acid and γ‐glutamyl transpeptidase in the bull reproductive tissues

Agrawal

Vanha‐Perttula

1988

Int J of Andrology

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“…Data in Table 3 indicate that glutamic acid is the principal free amino acid in buffalo bull seminal plasma. This agrees with the earlier reports on bull semen (MANN, 1964, AL-HAKIM et al, 1970and BROWN-WOODMAN and WHITE, 1974. The pattern of free amino acids i. e. the rank of their molar concentration, was glutamic acid followed by glycine, serine, alanine and aspartic acid.…”

Section: Discussionsupporting

confidence: 93%

Nitrogenous Constituents and Free Amino acid Content in the Seminal Plasma of Buffalo (Bos Bubalis) in Different Periods of the Year in Relation to Fertility

Chaudhary

Gangwar

2010

Zentralblatt Für Veterinärmedizin Reihe A

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Summary Semen of eight Murrah buffalo bulls was analysed for different nitrogenous constituents in different periods of the year and for the free amino acid content of seminal plasma. Total nitrogen, protein nitrogen, non‐protein nitrogen and amino acid nitrogen were found to be 649.10 ± 12.83, 532 ± 11.56, 116.26 ± 2 and 99.13 ± 4.4 mg. / 100 ml., of seminal plasma. All the nitrogen fractions vary significantly (P ≤ 0.01) between the bulls and between the periods. All the nitrogen fractions tended to be higher in colder periods as compared to the hot ones. Protein contributed 82 % of the total nitrogen. Conception rate was lowest during the hot period. NPN and amino acid nitrogen had a positive correlation with conception rate. Ten free amino acid‐aspartic acid, glutamic acid, serine, alanine, glycine, lysine, arginine, histidine and tyrosine (tryptophan) were identified by two dimensional TLC. Glutamic acid was the predominant amino acid (59.78 %) followed by glycine, serine, alanine and aspartic acid. Glutamic acid and glycine alone constituted 78.49 % of the total amino acids. Zusammenfassung Stickstoffhaltige Bestandteile und freie Aminosäuren im Samenplasma von Büffeln (Bos Bubalis) in Relation zur Fruchtbarkeit während verschiedener Jahreszeiten Bei 8 Murrah‐Büffeln wurde während verschiedener Jahreszeiten im Spermaplasma der Gehalt an stickstoffhaltigen Komponenten und freien Aminosäuren analysiert. Befunde (mg / 100 ml): Totalstickstoff 649,10 ± 12,83; Proteinstickstoff 532 ± 11,56; Nichtproteinstickstoff 116,26 ± 2; Aminosäurenstickstoff 99,13 ± 4,4. Alle N‐Fraktionen variierten signifikant sowohl von Tier zu Tier als auch von Jahreszeit zu Jahreszeit. Alle N‐Fraktionen zeigten in den kühlen Perioden steigende Tendenz im Vergleich zu den warmen Perioden. 82 % des N entfielen auf das Eiweiß. Während der warmen Periode war die Konzeptionsrate am kleinsten. Der NPN und Aminosäuren‐N zeigten positive Korrelationen zur Konzeptionsrate. Mittels der zweidimensionalen Dünnschichtchromatographie wurden 10 verschiedene Aminosäuren (Asparaginsäure, Glutaminsäure, Serin, Alanin, Glycin, Lysin, Arginin, Histidin, Tyrosin, Tryptophan) identifiziert. Die Glutaminsäure dominierte gegenüber den anderen Aminosäuren (59,78 %), gefolgt von Glycin, Serin, Alanin und Asparaginsäure. Glutaminsäure und Glycin machten 78,49 % aller Aminosäuren aus. Résumé Proportion azotée et des acides aminés libres dans le plasma séminal chez des buffles (Bos bubalis) par rapport à la fertilité durant les différentes époques de l'année On a analysé à différentes périodes de l'année le taux des composés azotés et des acides aminés libres dans le plasma séminal chez 8 buffles Murrah. Résultats en mg/100 ml: azote total 649,10 ± 12,83; azote protéique 532 ± 11,56; azote non protéique 116,26 ± 2; azote d'acides aminés 99,13 ± 4,4. Toutes les fractions N ont varié significativement aussi d'un animal à l'autre que d'une période de l'année à l'autre. Toutes les fractions N montrèrent une tendance à la hausse durant les périodes froides par rapport a...

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“…The hydrolysis of glutathione results in the release of L-glutamic acid, which is the most abundant amino acid in the seminal plasma of most mammals [6]. The postnatal increase of gamma-GT in epididymal caput and cauda is asso-ciated with a decline of its substrate GSH and an accumulation of the product L -G~u [l].…”

Section: Discussionmentioning

confidence: 99%

Gamma-Glutamyl Transpeptidase Activity in the Epididymis During Fetal and Postnatal Development in Rats

Hatier

Malaprade

Sabolovic

et al. 1991

Archives of Andrology

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The histochemical and biochemical distributions of gamma-glutamyl transpeptidase (gamma-GT) were investigated in the epididymis of rats during fetal and postnatal development. In the epididymal homogenates, gamma-GT activity was detected on the fifth day after birth. A sharp increase was observed after 30 days of life in the caput homogenates. Moderate levels of the enzyme were found in the cauda epididymis. Gamma-GT is histochemically detected from the 15th day of gestation in Wolffan ducts and in 17-to 18-day-old fetuses in newly differentiated epididymal tubules. Enzyme activity, was associated with the plasma membranes (apical, lateral, and basal), was preponderant on the apical part of the epithelial cells. During the first 15 days of the postnatal life, the histochemical reaction intensities were identical from the caput to the cauda epididymidis. From the 18th day onwards, enzyme activity decreased in the corpus and in the cauda, while gamma-GT increased in the caput epididymidis, and a strong activity was found on the apical surface of epithelial cells. Weak or moderate gamma-GT activity of spermatozoa in the caput tubules, increasing steadily from caput to cauda epididymidis, suggests that gamma-GT may be related to the functional maturation of spermatozoa.

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Amino Acid Composition of Semen and the Secretions of the Male Reproductive Tract

Cited by 20 publications

References 24 publications

Glutathione, L‐glutamic acid and γ‐glutamyl transpeptidase in the bull reproductive tissues

Glutathione, L‐glutamic acid and γ‐glutamyl transpeptidase in the bull reproductive tissues

Nitrogenous Constituents and Free Amino acid Content in the Seminal Plasma of Buffalo (Bos Bubalis) in Different Periods of the Year in Relation to Fertility

Gamma-Glutamyl Transpeptidase Activity in the Epididymis During Fetal and Postnatal Development in Rats

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