Glutathione, L‐glutamic acid and γ‐glutamyl transpeptidase in the bull reproductive tissues

Agrawal, Yash Pal; Vanha‐Perttula, T.

doi:10.1111/j.1365-2605.1988.tb00988.x

Cited by 41 publications

(24 citation statements)

References 24 publications

(16 reference statements)

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“…known that GGT is involved in the transfer of amino acids across the cellular membrane and in glutathione metabolism by transferring the glutamyl moiety to a variety of acceptor molecules including water, certain L-amino acids, and peptides, leaving the cysteine product to preserve intracellular homeostasis of oxidative stress (Alvarez & Storey 1989). It has been suggested that GGT also play an important role in the transfer of the glutathione residues to reactive groups on the sperm surface (Agrawal & Vanha-Perttula 1988). Therefore, the beneficial effect observed on sperm may be due to GGT protective activity against oxidative stress.…”

Section: Resultsmentioning

confidence: 99%

Influence of γ-glutamyltransferase and alkaline phosphatase activity on in vitro fertilisation of bovine frozen/thawed semen

Pero

Lombardi

Longobardi

et al. 2017

Italian Journal of Animal Science

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The aim of this work was to evaluate whether the residual amount of c-glutamyl-transferase (GGT) and alkaline phosphatase (ALP) in bovine sperm after freezing/thawing is correlated with fertility parameters, including blastocyst rates after in vitro fertilisation (IVF). The enzyme activities were determined in both spermatozoa and supernatant after centrifugation. While ALP was only correlated with sperm viability, GGT activity was correlated with sperm motility (r s ¼ .4; p < .05) both in sperm and supernatant. Interestingly, GGT activity was also correlated with cleavage (r s ¼ .5; p < .05 and .8; p < .01, for sperm and supernatant respectively) and blastocyst (r s ¼ .6 and .9, for sperm and supernatant respectively; p < .01) rates obtained after IVF. These results suggest that GGT could play an important role in the protection of sperm against oxidative stress and could be considered a reliable marker to assess frozen/thawed sperm quality in bovine.ARTICLE HISTORY

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Section: Resultsmentioning

confidence: 99%

Influence of γ-glutamyltransferase and alkaline phosphatase activity on in vitro fertilisation of bovine frozen/thawed semen

Pero

Lombardi

Longobardi

et al. 2017

Italian Journal of Animal Science

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“…Such a distribution was also observed in the bovine epididymal caput (Agrawal & Vanha-Perttula, 1988a). Strong evidence has also been presented that -GT in the rat epididymis is responsible for the high L-glutamic acid concentration in the luminal fluid (Agrawal & Vanha-Perttula, 1988b).…”

Section: Discussionmentioning

confidence: 66%

“…A similar enzyme distribution has previously been described for the bull (Agrawal & Vanha-Perttula, 1988a) and rat (Kohdaira et al, 1986;Agrawal & Vanha-Perttula, 1988b). Histochemically, -GT was most marked in the epithelium of the ductuli efferentes and of a specific area (segment 3) in the mouse epididymal caput.…”

Section: Discussionmentioning

confidence: 72%

“…Recent work indicates that the high levels of free L-glutamic acid in the mammalian epididymis result from the hydrolytic activity of -GT on GSH (Agrawal & Vanha-Perttula, 1988b). It has also been speculated that the transpeptidating activity of -GT may be involved in transferring L-glutamic acid residues to acceptors on the sperm surface with a resultant increase in the negative surface charge during the epididymal transit (Agrawal & Vanha-Perttula, 1988a (Agrawal & Vanha-Perttula, 1985, 1988b. Some tissues, such as different segments of the epididymis, were collected from both sides for the assay.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of -glutamyl transpeptidase in the mouse epididymis and its response to acivicin

Agrawal

Peura²,

Vanha‐Perttula³

1989

Reproduction

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Summary. \g=g\-Glutamyltranspeptidase (\g=g\-GT), its substrate (GSH) and hydrolytic product (l-glutamic acid) were measured biochemically in mouse reproductive tissues. The epididymal caput and seminal vesicles showed the highest specific activities of \g=g\-GT, while GSH and l-glutamic acid were widely distributed in all tissues. Histochemically, \g=g\-GTdisplayed a strong apical and supranuclear reaction and a moderate basal activity in the ductuli efferentes, a weak luminal reaction in the first, a moderate apical reaction in the second and a strong apical and supranuclear reaction in the third segment of the epididymal caput. In the epididymal corpus and cauda, the \g=g\-GT reaction was confined to the tubular lumina but an apical reaction was also present in the cauda. The daily administration of acivicin (12 mg/kg body weight), an irreversible inhibitor of \g=g\-GT, for 14 days resulted in a 60% suppression of the enzyme activity in the epididymal caput, while the \g=g\-GT inhibition in the kidney was >95%. The treatment caused no change in the activity of alanyl aminopeptidase. Histochemically, the basal and supranuclear \g=g\-GT activities in the ductuli efferentes and the third epididymal segment were suppressed, but the apical reactions were maintained. The in-vivo suppression of epididymal \g=g\-GTactivity may have implications in the control of post-testicular sperm maturation.

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“…Substantial quantities of GSH are found in the testis, reproductive tract fluids and epididymal spermatozoa (13) .…”

Section: Issn: 2320-5407mentioning

confidence: 99%

Effect of in Vitro Activation by Glutathione Supplemented Medium on Sperm Motility, Morphology and Vitality During Sperm Cryopreservation of Asthenozoospermic Men.

Amery¹,

Anbari²,

Mossa.³

2017

IJAR

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Background: Sperm cells from asthenozoospermia have humble motility, Glutathione is added in the medium to increase sperm motility and improve morphologically normal sperm. Cryopreservation of human spermatozoa is one of the best active and acceptable methods to preserve male fertility. However, cryopreservation may lead to changes in sperm structure and decline in sperm functional parameters. Objective: This study has been designed to investigate the effect of Glutathione on certain sperm functional parameters in Asthenozoospemic men before and after cryopreservation. Materials and Methods:The study included forty five asthenozoospermic infertile men. Ejaculated semen were obtained from patients and divided in to two equal portions, first portion was activated by free FertiCult Flushing medium as (control), second portion activated by Glutathione(GSH) in 5 mM concentration. The samples were cryopreserved in liquid nitrogen for one month. All the samples examined for sperm motility, morphology and vitality before and after cryopreservation-thawing, and comparison in these parameters between the two groups done. Results:In vitro activation by GSH resulted in significant (P<0.05) decrease in sperm concentration, highly significant increase (P<0.001) in sperm motility, highly significant decrease in immotile sperm (grade D) and significant increase (P<0.05) in morphologically normal sperm(MNS) compared with pre activation. After cryopreservationthawing, there was significant difference (P<0.05) in certain sperm functional parameters compared with control. Conclusion: There is positive effect and improvement in certain sperm functional parameters after in vitro sperm activation by GSH supplemented medium, before and after cryopreservation.

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Glutathione, L‐glutamic acid and γ‐glutamyl transpeptidase in the bull reproductive tissues

Cited by 41 publications

References 24 publications

Influence of γ-glutamyltransferase and alkaline phosphatase activity on in vitro fertilisation of bovine frozen/thawed semen

Influence of γ-glutamyltransferase and alkaline phosphatase activity on in vitro fertilisation of bovine frozen/thawed semen

Distribution of -glutamyl transpeptidase in the mouse epididymis and its response to acivicin

Effect of in Vitro Activation by Glutathione Supplemented Medium on Sperm Motility, Morphology and Vitality During Sperm Cryopreservation of Asthenozoospermic Men.

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