Influence of γ-glutamyltransferase and alkaline phosphatase activity on <i>in vitro</i> fertilisation of bovine frozen/thawed semen

Pero, Maria Elena; Lombardi, Pietro; Longobardi, V.; Boccia, Lorenzo; Vassalotti, Giuseppe; Zicarelli, L.; Ciani, Francesca; Gasparrini, B.

doi:10.1080/1828051x.2017.1290509

Cited by 11 publications

(11 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings are consistent with Pero et al. (2017) who reported a high concentration of GGT with higher sperm motility on frozen bovine semen. In addition, a significantly higher LDH and GGT concentration on frozen stallion semen extract can be observed in the present result.…”

Section: Discussionsupporting

confidence: 93%

Effects of cysteine and ascorbic acid in freezing extender on sperm characteristics and level of enzymes in post‐thawed stallion semen

Alamaary

Wahid

Hiew

et al. 2020

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Present study aimed to investigate the effect of adding antioxidants, cysteine and ascorbic acid on the levels of glutamic oxaloacetic transaminase (GOT), glutamic‐pyruvate (GPT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and γ‐glutamyl transpeptidase (GGT) enzymes of post‐thawed stallion sperm. Ten ejaculates were collected each from four healthy stallions and cryopreserved using HF‐20 freezing extender containing either 0 mg/ml cysteine or ascorbic acid, 0.5 mg/ml cysteine and 0.5 mg/ml ascorbic acid. All samples in freezing extender containing cysteine or ascorbic acid or none of them were assessed for sperm motility, viability, plasma membrane integrity, morphology and enzymes concentration. The ALP, LDH and GGT were significantly higher in 0‐group compared with cysteine and ascorbic acid groups. The sperm motility of frozen‐thawed semen with 0‐group was significantly better compared with cysteine and ascorbic acid groups. The variation on viability, sperm membrane integrity and morphology were insignificant between all treated groups. Therefore, these enzymes were reduced when using antioxidants in the freezing extender. Results of the present study suggest that concentration of ALP, LDH and GGT enzymes could be used as parameters for prediction of frozen‐thawed stallion semen.

show abstract

Section: Discussionsupporting

confidence: 93%

Effects of cysteine and ascorbic acid in freezing extender on sperm characteristics and level of enzymes in post‐thawed stallion semen

Alamaary

Wahid

Hiew

et al. 2020

Veterinary Medicine & Sci

View full text Add to dashboard Cite

show abstract

“…Interestingly, the inhibition of caspase activity was reported to prevent apoptosis and improve cryotolerance of mammalian cells (Stroh et al, ; Yagi et al, ). Likewise, an increased cryotolerance of porcine and bovine embryos was also recorded by inhibiting apoptosis using a caspase inhibitor, benzyloxycarbonyl‐Val‐Ala‐Asp‐fluoromethyl ketone (Z‐VAD‐FMK), during vitrification and subsequent culture (Men, Agca, Riley, & Critser, ; Pero et al, ). Z‐VAD‐FMK is a known pan‐caspase inhibitor that binds to the catalytic sites of caspases 3, 8 and 9, preventing the activation of the initiators (caspase 9 and 8) and/or the effector (caspase 3) of the apoptotic cascade.…”

Section: Introductionmentioning

confidence: 99%

Effect of caspase inhibitor Z‐VAD‐FMK on bovine sperm cryotolerance

Pagano

Longobardi

Canditiis

et al. 2020

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

The aim of this study was to evaluate the treatment of bovine semen with the pan‐caspase inhibitor benzyloxycarbonyl‐Val‐Ala‐Asp‐fluoromethyl ketone (Z‐VAD‐FMK), before or after freezing on semen quality. After the initial assessment, sperm from 4 bulls were pooled (Experiment 1) and cryopreserved in BioXcell containing 0, 20 and 100 μM Z‐VAD‐FMK. After thawing semen viability, motility, membrane integrity, as well as DNA fragmentation and ΔΨm were evaluated. In Experiment 2, bovine frozen/thawed sperm were incubated for 1 hr with 0, 20 and 100 µM Z‐VAD‐FMK before assessing the semen quality. The treatment with Z ‐VAD‐FMK before cryopreservation improved post‐thawing sperm motility compared to the control group (p < .05), while no differences were recorded in sperm viability and membrane integrity among groups (on average 86.8 ± 1.5 and 69.1 ± 1.4, respectively). Interestingly, at the highest concentration, DNA fragmentation decreased (p < .05), while the percentage of spermatozoa with high ΔΨm increased (p < .05). The results of Experiment 2 showed that 1‐hr treatment with Z‐VAD‐FMK did not affect sperm motility and viability (on average 63.4 ± 5.8 and 83.7.1 ± 1.2, respectively). However, Z‐VAD‐FMK improved sperm membrane integrity (p < .05) and at the highest concentration tested decreased the proportion of sperm showing DNA fragmentation (p < .05). No differences were recorded in the percentage of spermatozoa with high ΔΨm (on average 57.0 ± 11.4). In conclusion, the treatment with 100 µM of the caspase inhibitor Z‐VAD‐FMK before freezing increased bovine sperm mass motility and ΔΨm, while decreasing sperm DNA fragmentation. Treatment of semen after thawing with 100 µM Z‐VAD‐FMK improved sperm membrane integrity and reduced DNA fragmentation.

show abstract

“…This enzyme has been correlated with high sperm viability and motility in fresh samples from stallions (11). In bull, seminal GGT has been correlated with high sperm motility, embryo cleavage, and blastocyst rate with post-thawed sperm (40). In humans, low seminal concentrations of seminal GGT have been related to infertility (41).…”

Section: Discussionmentioning

confidence: 99%

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

et al. 2021

View full text Add to dashboard Cite

The Baudet du Poitou is a vanishing donkey breed recognized for engendering robust working mules. In Chile, only two pure breed Poitou males exist, which belong to the Chilean army and are used for mule production. We performed an extensive sperm and seminal analysis of these two jackasses aged 3 and 6 years and investigated the use of a simple hypometabolic extender for sperm cryopreservation. Computer-assisted sperm analysis showed high motility, velocity, and linearity in sperm movement. The seminal plasma analysis revealed that sodium and chloride were the main electrolytes, and globulins were the main metabolites. Active and variable enzymatic activity was observed. New information is reported about gamma-glutamyltransferase, aspartate aminotransferase, zinc, and magnesium concentrations in seminal plasma of Poitou donkeys. Ejaculates among jackasses showed some variability due to individual variability and different stages in sexual maturation according to age. The freezability index analysis based in viability, total motility and progressive motility with Botucrio extender (57.1 ± 11.0%; 56.6 ± 20.0%; and 22.6 ± 10.3%, respectively) were significantly higher (p < 0.05, p < 0.0001, and p < 0.0001, respectively) than with HM-0 extender (42,6 ± 11.4%; 14.9 ± 5.1%; and 1.0 ± 2.5%, respectively). We report new information on Poitou donkey semen and cryopreservation in the Southern Hemisphere that could be useful in donkey breeding and conservation programs to develop strategies that improve the effectiveness of population management of this breed.

show abstract

Influence of γ-glutamyltransferase and alkaline phosphatase activity on in vitro fertilisation of bovine frozen/thawed semen

Cited by 11 publications

References 11 publications

Effects of cysteine and ascorbic acid in freezing extender on sperm characteristics and level of enzymes in post‐thawed stallion semen

Effects of cysteine and ascorbic acid in freezing extender on sperm characteristics and level of enzymes in post‐thawed stallion semen

Effect of caspase inhibitor Z‐VAD‐FMK on bovine sperm cryotolerance

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

Contact Info

Product

Resources

About