Amino acid and vitamin determinations by TLC/HPTLC: review of the current state

Mohammad, Ali; Moheman, Abdul; Eldesoky, Gaber E.

doi:10.2478/s11532-012-0019-0

Cited by 26 publications

(22 citation statements)

References 83 publications

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“…[4][5][6][7][8][9] However, the ninhydrin method still has the disadvantage of limited sensitivity. Continual advances have been made by fluorescence detection of amino acids based on reactions with various derivatization reagents including OPA, 5 phenyl isothiocyanate (PITC), 27 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate (AQC).…”

Section: Resultsmentioning

confidence: 99%

“…3 Numerous chromatographic and electrophoretic methods have been developed for the separation and determination of amino acids. [4][5][6][7][8][9] Although these methods have high sensitivity for an individual amino acid, they usually require time-consuming separation procedures or specialized equipment. 10 Therefore, a simple and sensitive method for total amino acids detection without the separation of individual amino acid is a matter of concern.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Determination of Total Amino Acids Based on Surface-Enhanced Raman Scattering and Ninhydrin Derivatization

et al. 2017

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative Determination of Total Amino Acids Based on Surface-Enhanced Raman Scattering and Ninhydrin Derivatization

et al. 2017

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“…Among different chiral selectors used as the components of mobile phases in enantiomeric separation of d ‐ and l ‐amino acids, the most important is macrocyclic antibiotic (vancomycin). It has been found very useful as a mobile phase additive in the separation of dansyl‐ dl ‐amino acids on RP‐TLC plates (Bhushan and Parshad, ; Mohammad et al ., ).…”

Section: Thin‐layer Chromatographymentioning

confidence: 97%

Application of TLC, HPLC and GC methods to the study of amino acid and peptide enantiomers: a review

Dołowy

Pyka

2013

Biomedical Chromatography

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Amino acids are very important organic compounds in nature. The biological activity of amino acids depends mainly on their stereoisomeric configuration (d- or l-). Thus, the stereochemical analysis of amino acids and peptides is an important aspect of their characterization. Owing to the increasing role of amino acid configuration in biomedical and pharmaceutical studies, numerous analytical methods have been described in the literature. Among a wide range of analytical techniques available for the steroselective separation of different amino acids, which were obtained from plants or biological samples, chromatographic methods such as thin-layer chromatography, high-performance chromatography and also gas chromatography are very useful. This review presents such systems developed for direct stereoisomeric separation and quantitative determination of amino acid and peptide enantiomers with emphasis on selected literature published during two last decades. Almost all aspects, including sample preparation prior chromatographic analysis, stationary phase, solvent system and detection system, are discussed. New possibilities in chiral amino acid analysis have been opened up by the application of mass spectrometry and infrared detection in thin-layer chromatography and mass spectrometry-selected ion monitoring detection system in gas chromatography. A modern trend in high-performance chromatography is two-dimensional chromatography. These innovations have led to decreased development time and increased amino acid resolution and detectability.

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“…[8][9][10][11] According to the literature, the majority of TLC methods for the analysis of amino acids have been performed on silica gel layers as compared to other sorbent phases. Silica gel has been found harmful to humans, animals, and the environment.…”

Section: Introductionmentioning

confidence: 99%

“…[8][9][10][11] As one of the twelve principles of green chemistry stipulates the use of safer solvents and auxiliaries. [12] Therefore, there is a need to replace traditional organic solvents as previously mentioned by more environmentally 830 A.…”

Section: Introductionmentioning

confidence: 99%

On-Plate Identification of Amino Acids With Preliminary Separation Using Green Solvents

Mohammad

Siddiq

Moheman

et al. 2014

Journal of Liquid Chromatography & Related Technologies

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& A green thin layer chromatography (TLC) has been used as an analytical method for the identification and separation of amino acids through silica static flat bed in contact of n-butyl acetate, n-butyl alcohol, or ethylene glycol and their mixtures. The chromatographic system constituting silica gel as stationary phase, and n-butyl acetate-n-butyl alcohol-ethylene glycol in 3:5:2 ratio (v=v) as mobile phase with solvent ascent 5.0 cm was most favorable for on-plate identification of amino acids (lysine, alanine, and tryptophan) with preliminary separation. The presence of impurities such as metal cations, anions, and vitamins in the sample on the resolution of lysine, alanine, and tryptophan was also examined. The chromatographic parameters like DR F , separation factor (a), and resolution (Rs) of separated components of the mixture of these three amino acids were calculated. Densitogram of the separation of lysine, alanine, and tryptophan was recorded. The limit of detection for lysine was 0.6 mg spot À1 , whereas for alanine and tryptophan it was 1.0 mg spot À1 . The applicability of the proposed method was established by achieving separation from real and spiked pharmaceutical product. The proposed method is rapid and free from the use of volatile organic solvents and is therefore environmentally acceptable.

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Amino acid and vitamin determinations by TLC/HPTLC: review of the current state

Abstract: Abstract:© Versita Sp. z o.o.

Cited by 26 publications

References 83 publications

Quantitative Determination of Total Amino Acids Based on Surface-Enhanced Raman Scattering and Ninhydrin Derivatization

Quantitative Determination of Total Amino Acids Based on Surface-Enhanced Raman Scattering and Ninhydrin Derivatization

Application of TLC, HPLC and GC methods to the study of amino acid and peptide enantiomers: a review

On-Plate Identification of Amino Acids With Preliminary Separation Using Green Solvents

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