Quantitative Determination of Total Amino Acids Based on Surface-Enhanced Raman Scattering and Ninhydrin Derivatization

Sui, Huimin; Zhong, Hua; Han, Xiao; Zhang, Xiaolei; Wang, Xiaolei; Zhao, Bing

doi:10.2116/analsci.33.53

Cited by 9 publications

(3 citation statements)

References 32 publications

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“…912 Other amino acids generally lack clear spectroscopic signatures but can be detected via noncovalent interactions with a chemosensor. For example, fluorescent chemosensors bind with and detect a broad variety of amino acids, 913 Raman-active metal nanoparticles bind and detect amino acids via changes in the Raman spectral signals, 914 and electrochemical sensors detect the presence of amino acids via changes in their electrochemical signals (Figure 26). 915 4.7.…”

Section: Pharmaceutical Agentsmentioning

confidence: 99%

Supramolecular Luminescent Sensors

2018

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There is great need for stand-alone luminescence-based chemosensors that exemplify selectivity, sensitivity, and applicability and that overcome the challenges that arise from complex, real-world media. Discussed herein are recent developments toward these goals in the field of supramolecular luminescent chemosensors, including macrocycles, polymers, and nanomaterials. Specific focus is placed on the development of new macrocycle hosts since 2010, coupled with considerations of the underlying principles of supramolecular chemistry as well as analytes of interest and common luminophores. State-of-the-art developments in the fields of polymer and nanomaterial sensors are also examined, and some remaining unsolved challenges in the area of chemosensors are discussed. CONTENTS 4.4. Explosives 4.5. Pharmaceutical Agents 4.6. Biologically Relevant 4.6.1. Adenosine Triphosphate (ATP)

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Section: Pharmaceutical Agentsmentioning

confidence: 99%

Supramolecular Luminescent Sensors

2018

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“…The total polyphenol contents of dark tea were determined through the iron tartrate colorimetric method (22). Free amino acids were detected through ninhydrin colorimetry (23). The active ingredients in dark tea extract are listed in Supplementary Table 1.…”

Section: Active Ingredients In Dark Tea Extractmentioning

confidence: 99%

Defense against oxidative stress in Caenorhabditis elegans by dark tea

Wang,

Zhang,

Zhang

et al. 2024

Front. Vet. Sci.

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Dark tea, rich in nutricines including tea polyphenols and free amino acids, is a kind of post-fermented tea. The potential application of nutricines against oxidative damage and senescence, which drives animal health maintenance and disease prevention, has attracted considerable interest. In this study, the effect of dark tea and its effects on longevity and defense against oxidative stress was investigated in the Caenorhabditis elegans (C. elegans) model. Under normal conditions, dark tea extended the lifespan without significant impairment of propagation. It also improved the motility, alleviated the fat accumulation and apoptosis. Additionally, orally administered dark tea could significantly decrease the level of reactive oxygen species (ROS) and resulted in a superior lifespan in H2O2-induced oxidative stressed C. elegans. In antioxidant assays in vitro, dark tea was found to be rich in strong hydroxyl, DPPH and ABTS+ free radical scavenging capacity. Interestingly, mRNA sequence analyses further revealed that dark tea may catalyze intracellular relevant oxidative substrates and synthesize antioxidants through synthetic and metabolic pathways. These results suggest that dark tea is worth further exploration as a potential dietary supplement for the maintenance of animal health and the prevention of related diseases.

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“…The ninhydrin method was used to determine the free amino acids (Sui et al, 2017). The ninhydrin reagent reacts with the amino acids in the samples to form a purple color, and the free amino acids were estimated colorimetrically at 570 nm of absorbance.…”

Section: Soluble Sugars and Free Amino Acidsmentioning

confidence: 99%

Impacts of Cobalt, Selenium and Silicon Biofortification on the Growth, Productivity and Nutritional Value of Lettuce

Abdalla,

Mousa,

Ibrahim

et al. 2024

Egyptian Journal of Horticulture

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R ECENTLY, beneficial elements have received much attention because of their pivotal contribution in crop sustainability. The biofortification of plants with these elements is one of the brilliant strategies that provide these elements in the food chain for human consumption. Additionally, an unprecedented approach has been made to study the impact of the beneficial elements on plant growth, development, yield, and quality. Accordingly, this study was conducted to investigate the separate efficacy of the cobalt, selenium, and silicon on the biofortification of lettuce cv. Limor plants and scrutinize their potential impacts on growth and productivity during the 2020 and 2021 seasons. Ten foliar treatments, including cobalt sulphate at 100, 200 and 300 μM, sodium selenite at 10, 20 and 30 μM, and sodium silicate at 0.5, 1 and 2 mM, and distilled water as a control treatment. The experimental design was a completely randomized block with three replicates. Results clarified that the spraying of these beneficial elements significantly enhanced the vegetative growth parameters, mineral content, chlorophyll content, sugars and free amino acids as compared to the control. The treatments did not affect the content of nitrate and nitrite in the lettuce heads. The increases in head fresh weight ranged from 11.35 to 14.54% in the first season, and from 13.07 to 16.95% in the second season. In conclusion, supplying cobalt sulphate at 100 μM, sodium selenite at 20 μM or sodium silicate at 2 mM as foliar sprayings not only improves the vegetative growth and yield of lettuce plants but also enhances the nutritional quality of plants and fortifies the content of these elements in the lettuce heads.

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Quantitative Determination of Total Amino Acids Based on Surface-Enhanced Raman Scattering and Ninhydrin Derivatization

Cited by 9 publications

References 32 publications

Supramolecular Luminescent Sensors

Supramolecular Luminescent Sensors

Defense against oxidative stress in Caenorhabditis elegans by dark tea

Impacts of Cobalt, Selenium and Silicon Biofortification on the Growth, Productivity and Nutritional Value of Lettuce

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