Ameliorative effect of protein and calcium on fluoride-induced hepatotoxicity in rabbits

Abstract

To investigate whether protein (Pr) or calcium (Ca) supplementation could ameliorate hepatic damage induced by excessive fluoride (F); thirty-two 30-day-old healthy New Zealand rabbits were randomly divided into four groups (female: male = 1:1). The four groups were maintained on distilled water and fed the following diets for 120 days: (1) a malnutrition control (MC) diet (8.58% Pr, 0.49% Ca); (2) the MC diet plus HiF (high fluoride in their diet, 200 mg F ion/kg from NaF); (3) a Ca deficient MC diet plus HiPr+HiF (0.46% Ca, 18.41% Pr, plus HiF); and (4) a Pr deficient MC diet plus HiCa+HiF (2.23% Ca, 8.35% Pr, plus HiF). Results show that in HiF group, the serum total Pr (TPr) and albumin (ALB) content significantly decreased, whereas both Pr and Ca rich diets significantly enhanced their levels. In liver, low superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, high malondialdehyde (MDA) content, and evident mitochondria lesions in HiF group indicated a significant oxidative stress, while Pr or Ca supplementation brought an ultrastructural repair and a recovery antioxidant defense in liver. The findings in the present work implied the ameliorative effects of Pr or Ca supplementation on F-induced hepatotoxicity in rabbits.

Effects of Maalox Plus® Antacid and PureCal® Calcium Supplement on Physical Characteristics, Body Weight, Tissue Minerals and Histopathology of Rats Subjected to Alcohol Intoxication

“…Similar findings of an earlier study have seen MgSO 4 combating inflammatory responses and oxidative damage that are experienced when suffering from cholestasis liver injury [53]. The ameliorative properties of calcium on hepatotoxicity induced by fluoride on rabbit subjects have also been demonstrated [54], where just like with magnesium, calcium was able to increase the activity of superoxide dismutase, glutathione peroxidase and brought about ultra-structural repair of the liver. It is thus evident that both elements have a curative effect on liver pathophysiology and that it was the 1:2 ratio of Mg:Ca that turned out to be more effective in repairing the liver architecture.…”

Effect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosis

“…37 Many studies have shown that adequate or supplementary ingestion of Pr or Ca can reduce toxic effects of F on the ribs and femurs of rabbits, 21,38 and the thyroid, kidney and thymus of rats, 12,41,42 and the liver and immune functions of rabbits. [43][44][45] The question then arises: whether Pr and Ca will affect the expression of COL and DPP in F? A model of normal nutrition and malnutrition can be set up to observe the effect of F on the gene and protein expressions of COL I and DPP in incisors in rats by quantitative real-time reverse transcription polymerase chain reaction (QRT-PCR) and immunohistochemistry (IHC).…”