Ameliorative effect of chitosan-conjugated 17α-methyltestosterone on testicular development in Clarias batrachus

Saha, Ratnadeep; Bhat, Irfan Ahmad; Ravi, Charan; Purayil, Suresh Babu Padinhate; Krishna, Gopal; Pavan‐Kumar, A.; Sharma, Rupam

doi:10.1016/j.anireprosci.2018.04.078

Cited by 14 publications

(9 citation statements)

References 37 publications

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“…In this study, we aimed to evaluate the efficiency of the Ovsynch estrous synchronization protocol as one of the most crucial estrous synchronization protocols in goats when GnRH and PGF 2α are delivered using a nano-based drug delivery system. Several studies have reported the advantages of engineered nanodrugs, including hormones, and their efficient biological activities due to the changes in physicochemical properties of materials in nanoforms [ 18 , 19 ]. Engineered nanohormones have a longer half-life time and sustained release, greater cellular uptake, and more efficient passage across epithelial or endothelial barriers [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

Nanodelivery System for Ovsynch Protocol Improves Ovarian Response, Ovarian Blood Flow Doppler Velocities, and Hormonal Profile of Goats

Hashem

El‐Sherbiny

Fathi

et al. 2022

Animals

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Fifteen cyclic, multiparous goats were equally stratified and received the common Ovsynch protocol (GPG: intramuscular, IM, injection of 50 mg gonadorelin, followed by an IM injection of 125 µg cloprostenol 7 days later, and a further IM injection of 50 mg gonadorelin 2 days later) or the Ovsynch protocol using nanofabricated hormones with the same dosages (NGPG) or half dosages (HNGPG) of each hormone. The ovarian structures and ovarian and luteal artery hemodynamic indices after each injection of the Ovsynch protocol using B-mode, color, and spectral Doppler scanning were monitored. Levels of blood serum progesterone (P4), estradiol (E2), and nitric oxide (NO) were determined. After the first gonadotrophin-releasing hormone (GnRH) injection, the number of large follicles decreased (p = 0.02) in NGPG and HNGPG, compared with GPG. HNGPG resulted in larger corpus luteum (CL) diameters (p = 0.001), and improved ovarian and luteal blood flow, compared with GPG and NGPG. Both NGPG and HNGPG significantly increased E2 and NO levels compared with GPG. HNGPG increased (p < 0.001) P4 levels compared with GPG, whereas NGPG resulted in an intermediate value. After prostaglandin F2α (PGF2α) injection, HNGPG had the largest diameter of CLs (p = 0.001) and significantly improved ovarian blood flow compared with GPG and NGPG. Both NGPG and HNGPG increased (p = 0.007) NO levels, compared with GPG. E2 level was increased (p = 0.028) in HNGPG, compared with GPG, whereas NGPG resulted in an intermediate value. During the follicular phase, HNGPG increased (p = 0.043) the number of medium follicles, shortened (p = 0.04) the interval to ovulation, and increased (p < 0.001) ovarian artery blood flow and levels (p < 0.001) of blood serum P4, E2, and NO, compared with GPG and NGPG. During the luteal phase, the numbers of CLs were similar among different experimental groups, whereas the diameter of CLs, luteal blood flow, and levels of blood serum P4 and NO increased (p < 0.001) in HNGPG, compared with GPG and NGPG. Conclusively, the nanodelivery system for the Ovsynch protocol could be recommended as a new strategy for improving estrous synchronization outcomes of goats while enabling lower hormone dose administration.

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Section: Discussionmentioning

confidence: 99%

Nanodelivery System for Ovsynch Protocol Improves Ovarian Response, Ovarian Blood Flow Doppler Velocities, and Hormonal Profile of Goats

Hashem

El‐Sherbiny

Fathi

et al. 2022

Animals

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“…Three types of er (era, erb1 and erb2) are found to regulate spermatogenesis and are expressed in testis and in germ cells (Menuet et al 2002). Androgens such as testosterone and 11-KT strongly regulate testicular development, steroidogenesis and spermatogenesis in fish (Schulz et al 2010;Saha et al 2018). Androgens downregulate the amh expression which is a negative regulator of spermatogenesis in fish (Schulz et al 2010).…”

Section: Molecular Regulation Of Spermatogenesis In Fishmentioning

confidence: 99%

“…2010; Saha et al . 2018). Androgens downregulate the amh expression which is a negative regulator of spermatogenesis in fish (Schulz et al .…”

Section: Molecular Regulation Of Spermatogenesis In Fishmentioning

confidence: 99%

Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Bhat

Dar

Ahmad

et al. 2021

Reviews in Aquaculture

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In fish, as in higher animals, the normal ontogenesis of the gonads is the basis for successful reproduction. Gonadal differentiation and development depend on the genetic cascade that programmes the events. Eventually, gonads become the sites for the maturation of gametes, which are originally derived from the primordial germ cells (PGCs) and form the cellular basis of reproduction. In fish hormones of the hypothalamic-pituitary-gonadal (HPG) axis regulate reproduction and fertility by promoting or inhibiting the production of gonadal sex steroids and their function. Gonadal sex steroids not only guide proper gonadal development and function but also play a key role in the activation and regulation of the HPG axis through two feedback control systems. In this review, we discuss the general outline and expression pattern of the major genes involved in testicular development and gonadal steroidogenesis in fish. The review attempts, for the first time, to collect and discuss recent information regarding the regulation of sex differentiation and steroidogenic genes during the stages of testicular development and in response to different external factors. Furthermore, clear insights into the important genes and their regulation involved in the HPG axis and spermatogenesis are presented briefly. This review can serve as a guide for fish breeders on the substances that have a positive impact on reproduction in males.

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“…The use of nano-drug, or nano-hormone delivery systems (NHDS) may be useful for alleviating the incidence of these factors. Firstly, enhancing the biological efficiency of the hormonal treatment; conjugation of the hormone with a suitable nano-particle is hypothesized to increase its half-life, improve its passage across epithelial or endothelial barriers into blood or lymph circulation and sustain its delivery to the target sites, improving their uptake by cells [35]; hence, it is possible to use lower doses. As an example, gonadotrophin releasing hormone (GnRH) is included in some prostaglandin-based protocols, since it induces ovulation and has luteotrophic effects favoring progesterone secretion and the maintenance of pregnancy [36].…”

Section: Nano-hormone Delivery Systems and Cycle Managementmentioning

confidence: 99%

State-of-the-Art and Prospective of Nanotechnologies for Smart Reproductive Management of Farm Animals

Hashem

González-Bulnes

2020

Animals

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Many biotechnological assisted reproductive techniques (ART) are currently used to control the reproductive processes of farm animals. Nowadays, smart ART that considers technique efficiency, animal welfare, cost efficiency and environmental health are developed. Recently, the nanotechnology revolution has pervaded all scientific fields including the reproduction of farm animals, facilitating certain improvements in this field. Nanotechnology could be used to improve and overcome many technical obstacles that face different ART. For example, semen purification and semen preservation processes have been developed using different nanomaterials and techniques, to obtain semen doses with high sperm quality. Additionally, nanodrugs delivery could be applied to fabricate several sex hormones (steroids or gonadotrophins) used in the manipulation of the reproductive cycle. Nanofabricated hormones have new specific biological properties, increasing their bioavailability. Applying nanodrugs delivery techniques allow a reduction in hormone dose and improves hormone kinetics in animal body, because of protection from natural biological barriers (e.g., enzymatic degradation). Additionally, biodegradable nanomaterials could be used to fabricate hormone-loaded devices that are made from non-degradable materials, such as silicon and polyvinyl chloride-based matrixes, which negatively impact environmental health. This review discusses the role of nanotechnology in developing some ART outcomes applied in the livestock sector, meeting the concept of smart production.

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Ameliorative effect of chitosan-conjugated 17α-methyltestosterone on testicular development in Clarias batrachus

Cited by 14 publications

References 37 publications

Nanodelivery System for Ovsynch Protocol Improves Ovarian Response, Ovarian Blood Flow Doppler Velocities, and Hormonal Profile of Goats

Nanodelivery System for Ovsynch Protocol Improves Ovarian Response, Ovarian Blood Flow Doppler Velocities, and Hormonal Profile of Goats

Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

State-of-the-Art and Prospective of Nanotechnologies for Smart Reproductive Management of Farm Animals

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