Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Bhat, Irfan Ahmad; Dar, Jaffer Yousuf; Ahmad, Irshad; Mir, Ishfaq Nazir; Bhat, Hussna; Bhat, Raja Aadil Hussain; Ganie, Parvaiz Ahmad; Sharma, Rupam

doi:10.1111/raq.12563

Cited by 16 publications

(14 citation statements)

References 318 publications

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“…In the testes, these neurotransmitters can trigger a series of syntheses and secretion of sex steroids regulated by the HPG axis. Moreover, sex steroids produced by the testes feedback both positively and negatively to the brain, which in-turn finely controls gonadotropin secretion ( 74 ). In this study, several genes involved in steroidogenesis in the testes of common carp were identified, including cytochrome P450 family 11 subfamily b ( cyp11b ), hydroxysteroid 11β-dehydrogenase 2 ( hsd11b2 ), and hydroxysteroid 17-β dehydrogenase 3 ( hsd17b3 ) ( Figure 9 ).…”

Section: Discussionmentioning

confidence: 99%

Dynamic Gene Expression and Alternative Splicing Events Demonstrate Co-Regulation of Testicular Differentiation and Maturation by the Brain and Gonad in Common Carp

et al. 2022

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The common carp (Cyprinus carpio) accounts for approximately 10% of the annual freshwater aquaculture production and is an ideal model to study cyprinidae reproduction. Female common carp grow faster than the males; therefore, related research presents an opportunity with high application value. Although we have a detailed understanding of common carp’s early gonadal differentiation process, information about genome-wide gene expression, regulation, and underlying molecular mechanisms during this process remain limited. Here, time-course data comprising six key stages during testicular differentiation and maturation were investigated to further understand the molecular mechanisms underlying the testicular development in cyprinid species. After integrating these time-series data sets, common carp genome, including 98,345 novel transcripts and 3,071 novel genes were re-annotated and precisely updated. Gene co-expression network analysis revealed that the ubiquitin-mediated proteolysis pathway was essential for metabolism during testicular differentiation in the endocrine system of C. carpio. Functional enrichment analyses indicated that genes mainly related to amino acid metabolism and steroid hormone synthesis were relatively highly expressed at the testicular undifferentiation stages, whereas genes associated with cell cycle and meiosis were expressed from the beginning of testicular differentiation until maturation. The dynamics of alternative splicing events demonstrated that exon skipping accounted for majority of the alternative splicing events in the testis and the brain during gonad development. Notably, several potential male-specific genes (fanci and sox30) and brain-specific genes (oxt, gad2, and tac1, etc.) were identified. Importantly, we traversed beyond the level of transcription to test for stage- and gonad-specific alternative splicing patterns between the brain and testis. This study is the first to describe a comprehensive landscape of alternative splicing events and gene expression patterns during gonadogenesis in common carp. This work is extremely valuable to elucidate the mechanisms underlying gonadal differentiation in Cyprinidae as well as other fish species.

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Section: Discussionmentioning

confidence: 99%

Dynamic Gene Expression and Alternative Splicing Events Demonstrate Co-Regulation of Testicular Differentiation and Maturation by the Brain and Gonad in Common Carp

et al. 2022

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“…Similarly, the transcript profile of some important transcription factors required in SD and gonadal development in fish were analyzed during embryogenesis in A. salmon. The genes included gonadal somatic derived factor ( gsdf ), doublesex and mab-3 related transcription factor ( dmrt1 )), anti-Mullerian hormone ( amh ), forkhead box protein L2 ( foxl2 ) and cytochrome P450arom ( cyp19a ) [ 41 , 42 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into Early Ontogenesis of Salmo salar: RNA Extraction, Housekeeping Gene Validation and Transcriptional Expression of Important Primordial Germ Cell and Sex-Determination Genes

Bhat

Dubiel

Rodrı́guez³

et al. 2023

Animals

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The challenge in extracting high-quality RNA impedes the investigation of the transcriptome of developing salmonid embryos. Furthermore, the mRNA expression pattern of important PGC and SD genes during the initial embryonic development of Salmo salar is yet to be studied. So, in the present study, we aimed to isolate high-quality RNA from eggs and developing embryos to check vasa, dnd1, nanos3a, sdf1, gsdf, amh, cyp19a, dmrt1 and foxl2 expression by qPCR. Additionally, four HKGs (GAPDH, UB2L3, eEf1a and β-actin) were validated to select the best internal control for qPCR. High-quality RNA was extracted, which was confirmed by spectrophotometer, agarose gel electrophoresis and Agilent TapeStation analysis. UB2L3 was chosen as a reference gene because it exhibited lower intra- and inter-sample variation. vasa transcripts were expressed in all the developmental stages, while dnd1 was expressed only up to 40 d°C. Nanos3a was expressed in later stages and remained at its peak for a shorter period, while sdf1 showed an irregular pattern of mRNA expression. The mRNA expression levels of SD genes were observed to be upregulated during the later stages of development, prior to hatching. This study presents a straightforward methodology for isolating high-quality RNA from salmon eggs, and the resulting transcript profiles of significant PGC and SD genes in S. salar could aid in improving our comprehension of reproductive development in this commercially important species.

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“…Vertebrate reproduction requires a myriad of precisely orchestrated events, including the production of oocytes in females and the production of sperm in males. Sperm are unique cells, produced through the complex process of spermatogenesis, in which there are a number of checkpoints in place to guarantee delivery of a high-quality product (Bhat et al, 2021). Gamete quality has become a focus in aquaculture and fish population studies, due to its impact on fertilization success and larval survival.…”

Section: Introductionmentioning

confidence: 99%

Sperm collection and computer-assisted sperm analysis in the teleost model Japanese medaka (Oryzias latipes)

Closs

Sayyari

Fontaine

2022

Preprint

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Japanese medaka (Oryzias latipes) are a teleost fish and an emerging vertebrate model for ecotoxicology, developmental, genetics, and physiology research. Medaka are also used extensively to investigate vertebrate reproduction, which is an essential biological function as it allows a species to perpetuate. Sperm quality is an important indicator of male fertility and thus reproduction success. Techniques for extracting sperm and sperm analysis are well documented for many species, including for teleost fish. Collecting semen is relatively simple in larger fish but can be more complicated in small model fish as they produce less sperm and are more delicate. This article therefore describes two methods of sperm collection in the small model fish Japanese medaka: testes dissection and abdominal massage. We demonstrate that both approaches are viable for medaka and show that abdominal massage can be performed a repeated number of times as the fish quickly recover from the procedure. We also describe a protocol for computer-assisted sperm analysis in medaka to objectively assess several important indicators of medaka sperm quality (motility, progressivity, duration of motility, relative concentration). The use of these procedures combined with the other advantages of using this small teleost model will greatly improve the understanding of the environmental, physiological, and genetic factors influencing fertility in vertebrate males.

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Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Cited by 16 publications

References 318 publications

Dynamic Gene Expression and Alternative Splicing Events Demonstrate Co-Regulation of Testicular Differentiation and Maturation by the Brain and Gonad in Common Carp

Dynamic Gene Expression and Alternative Splicing Events Demonstrate Co-Regulation of Testicular Differentiation and Maturation by the Brain and Gonad in Common Carp

Insights into Early Ontogenesis of Salmo salar: RNA Extraction, Housekeeping Gene Validation and Transcriptional Expression of Important Primordial Germ Cell and Sex-Determination Genes

Sperm collection and computer-assisted sperm analysis in the teleost model Japanese medaka (Oryzias latipes)

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