Ameliorative effect of Annona reticulata L. leaf extract on antihyperglycemic activity and its hepato-renal protective potential in streptozotocin induced diabetic rats

Abstract

Background Annona reticulata L. is a traditionally important plant due to its versatile source of medicine and industrial products. It is used to treat cardiac problems, wound healing, diabetes, ulcers and bacterial infections. As it is a commercial fruit bearing plant, wide range studies on this plant reaches the mankind efficiently. Objective(s) The present study was focussed on antihyperglycemic potential of A. reticulata leaves under in vitro and in vivo . Material and methods The in vitro phytochemical analysis, total phenolic, flavonoid content, inhibition activity on alpha amylase and alpha glucosidase enzymes were determined for various solvent extracts, followed by in vivo oral toxicity, short term, dose dependant antihyperglycemic studies, oral glucose tolerance tests were performed. The activity of methanolic extract of A.reticulata (MeEAR)-500 mg/kg b.wt was studied for 28 days in diabetic rat model. Histopathological examinations and serum biochemical assays were performed. Gas chromatography–Mass spectrometry (GC–MS) analysis was performed to identify the compounds present in MeEAR. Results Among the various extracts, MeEAR possesses higher amount of phenols and flavonoids with effective inhibition on carbohydrate hydrolysing enzymes (P < 0.05) and also exhibited higher glycemic control in vivo , with simultaneous improvement in the hepatic and renal activities in diabetic rats. GC–MS analysis revealed the presence of 63 bioactive compounds including carboxylic-acids, alcoholic groups, fattyacid esters, amino acid derivatives. Conclusion Altogether, our study demonstrated that leaves of A.reticulata possess better antihyperglycemic activity and could be developed in to a potential antidiabetic drug with further studies.

Preventive and Ameliorative Effects of Diet Supplemented with Cucurbita maxima Leaf on Hyperglycemia and Hepatotoxicity in STZ-Induced Diabetic Rats

“…In the current investigation, hepatocyte injury was indicated by a substantial rise in liver function parameters (p<0.05) in the serum of the STZ-induced diabetic control rat group which collaborated with the report of Pulivarthi et al 39 . According to Al-Bahrani 40 and Saleh et al 38 , high levels of these enzymes in the serum of STZ-induced diabetic rats represent hepatocyte damage.…”

Aqueous extract of Polygonatum sibiricum ameliorates glucose and lipid metabolism via PI3K / AKT signaling pathway in high‐fat diet and streptozotocin‐induced diabetic mice

“…It is reported that 463 million people are suffering from diabetes mellitus around the world until 2019 (IDF, 2019) while the main patient is type 2 diabetes mellitus (T2DM), which accounts for 90% of all diabetic cases (Lee & Li, 2021). T2DM is principally caused by insulin resistance (IR) and insulin secretion disorders and IR is believed as the initiating factor in the development of T2DM (American Diabetes Association, 2016; Pulivarthi & Naidu, 2021). When IR occurs in the body, islet beta cells will facilitate insulin production to compensate for IR which to maintain blood glucose level (Diabetes Care, 2015).…”