Ameliorative activity of medicinal plant fraction for neuroprotection against acrylamide-induced neurotoxicity in Drosophila melanogaster—a comparative study

Abstract

Background Medicinal plant extracts used in folk medicine seem to be crucial since those are generally accepted by people without worrying about the toxicity. In our present study, we have compared the neuroprotective role of the rhizome of Curculigo orchioides Gaertn. and leaf extracts of Olea dioica Roxb., against acrylamide-induced neurotoxicity in Drosophila melanogaster. Results In-vivo neurotoxic study was carried out using 7-day-old flies (wild-type D. melanogaster). Prior to co-after exposing the flies with acrylamide (8 mM) along with or without OLE-2 and CU-3 fractions (0.2, 0.4 and 0.6% w/v), the heads of flies of both the control and treated groups were homogenized for biochemical assay. ACR-treated groups have shown higher elevation in AChE, SOD, LPO, and CAT activity when compared to control and treated (plant fraction) groups. Biochemical and histopathology studies show that both the plant fractions (OLE-2 and CU-3) have neuroprotective action against acrylamide. Conclusion The present study has demonstrated that dietary supplementation with plant fraction of OLE-2 and CU-3 has increased antioxidant enzymes and decreased AChE activity in D. melanogaster. This activity could be part of the probable mechanisms of action by which OLE-2 and CU-3 fractions have extended the lifespan and locomotory activity in fruit flies. These fruit flies continue to provide an exciting system for metabolic studies and should be more extensively exploited. Based on these results, further studies on the identified bioactive compounds from these two plants are being planned.

In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

“…The extracts demonstrated strong AChE inhibition, with values > 60% at various sample concentrations (10, 20, 40, 60, 80, and 100 μg/mL). The phenolic compounds are known to be neuroprotective exhibiting AChE inhibitory activity and could be one of the main approaches for the treatment of AD ( Pratap and Shantaram, 2020b ) ( Pratap et al, 2021 ). The neuroprotective action of the methanolic extract of Olea dioica Roxb.…”

“…According to earlier research on the chemical makeup of various natural compounds, curcumin, Gallic acid, Diterpene, Apigenin, Luteolin, and others are essential for cell division, anti-inflammatory activity, neuroprotection, and anti-amyloidogenic activity. They also act as pro-oxidants ( Forni et al, 2019 ) ( Pratap et al, 2021 ; Gk et al, 2022 ). According to Haddadi et al (2014) , AChE contributes significantly to the aging process by promoting the production of highly neurotoxic amyloid-b fibrils and AChE-Aβ complexes.…”

“…The fruits of Olea dioica are used to cure skin diseases ( Yesodharan & Sujana, 2007 ). The plant extract of Olea dioica exhibits neuroprotective effects in Drosophila against acrylamide-induced neurotoxicity ( Pratap et al, 2021 ). According to a literature survey, Olea dioica Roxb, is a very important ethnomedicinal plant owing several pharmacological activities and could be a source of anti-AChE and anti-amyloidogenic compounds which could be a promising candidate for creating new drug lead for the controlling progression of AD in humans.…”

Inhibition of phosphodiesterase: A novel therapeutic target for the treatment of mild cognitive impairment and Alzheimer’s disease

“…However, the synthetic PDE inhibitors adverse effects have limited their usage in clinical practice. Natural products ingredients are widely accepted and regarded harmless ( Pratap and Shantaram, 2020 ; Pratap et al, 2021 ). Several plant derived components have been investigated pre-clinically for PDE inhibitory action throughout the last decade ( Temkitthawon et al, 2011 ), and they were proved to be as effective as synthetic drugs in inhibiting PDEs ( Figure 4 ).…”