Neuroprotective, lifespan and memory enhancing potential, and molecular docking studies of natural compound from Curculigo orchioides: A study on Alzheimer's disease model of Appl-GAL4 Drosophila melanogaster

GK, Pratap; Nagaraju, Pramod G; Danagoudar, Ananda; Joshi, Chandrashekhar G.; CG, Poornima Priyadarshini; Mohammed, Yasser Hussein Eissa; Kumar, L.; Shantaram, Manjula

doi:10.1016/j.sajb.2022.05.047

Cited by 3 publications

(3 citation statements)

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“…According to earlier research on the chemical makeup of various natural compounds, curcumin, Gallic acid, Diterpene, Apigenin, Luteolin, and others are essential for cell division, anti-inflammatory activity, neuroprotection, and anti-amyloidogenic activity. They also act as pro-oxidants ( Forni et al, 2019 ) ( Pratap et al, 2021 ; Gk et al, 2022 ). According to Haddadi et al (2014) , AChE contributes significantly to the aging process by promoting the production of highly neurotoxic amyloid-b fibrils and AChE-Aβ complexes.…”

Section: Discussionmentioning

confidence: 99%

“…Instruments for LC-MS (Agilent 1100 LC, Bruker-make MS model Esquire 3000) having an ESI (electrospray ionisation), an APCI (atmospheric pressure chemical ionisation), and a PDA detection source with a mass range of 100 amu in quadrupole and 10,000 amu ( Jain et al, 2016 ). The mass-charge ratio and molecular mass of the methanolic fractions were determined by using the spectrum database for organic compounds in MassBank, which was then utilised to identify the mass fragmentations ( Pratap et al, 2020b ; Gk et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…The sample was formed by uniformly dispersing the methanolic fractions within a matrix of KBr ( Owokotomo et al, 2015 ). The intensity bands were compared to standard values in order to identify the functional groups ( Pratap et al, 2020b ; Gk et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

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In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Kenchappa,

Karthik,

Vijendra

et al. 2023

Front. Pharmacol.

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Alzheimer’s disease (AD) is a type of neurodegenerative disease, associated with the hastening of ROS, acetylcholinesterase (AChE) activity, and amyloid β peptides plaques in the brain. The limitations and side effects of existing synthetic drugs incline toward natural sources. In the present communication active principles of methanolic extract of Olea dioica Roxb, leaves are explored as an antioxidant, AChE inhibitor, and anti-amyloidogenic. Furthermore, neuroprotection against the amyloid beta-peptide has been studied. The bioactive principles were identified by GC-MS and LC-MS and further subjected to antioxidant (DPPH and FRAP) and neuroprotection (AChE inhibition, ThT binding, and MTT assay, DCFH-DA and lipid peroxidation (LPO) assay using neuroblastoma (SHSY-5Y) cell lines) assays. Methanolic extract of O. dioica Roxb, leaves was found to contain polyphenols and flavonoids. In vitro assays exhibited potential antioxidant and anti-AChE (˃50%) activities. ThT binding assay indicated protection against amyloid-beta aggregation. MTT assay, Aβ1-40 (10 µM) with extract increase the cell viability (˃50%) and showed significant cytotoxicity to SHSY-5Y cells. ROS level (˃25%) significantly decreased in the Aβ1-40 (10 µM) + extract (15 and 20 μM/mL) and LPO assay (˃50%) suggesting prevention of cell damage. Results advocate that O. dioica leaves are a good source of antioxidants, anti-AChE, and anti-amyloidogenic compounds which may be further evaluated as a natural medicine for the treatment of AD.

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