Ameliorating effects of two extracts of Nigella sativa in middle cerebral artery occluded rat

Abstract

Purpose:Aqueous and hydroalcoholic extracts of Nigella sativa (400 mg/kg, orally) for 7 days were administered and evaluated for their neuroprotective effects on middle cerebral artery occluded (MCAO) rats.Materials and Methods:Cerebral ischemia was induced by middle cerebral artery occlusion for 2 h followed by reperfusion for 22 h. After 24 h of ischemia, grip strength, locomotor activity tests were performed in the surgically operated animals. After behavioral tests, animals were immediately sacrificed. Infarct volumes followed by the estimation of markers of oxidative stress in the brains were measured.Results:Locomotor activity and grip strength of animals were improved in both aqueous and hydroalcoholic extracts pretreated rats. Infarct volume was also reduced in both extracts pretreated rats as compared with MCAO rats. An elevation of thiobarbituric acid reactive substance (TBARS) and a reduction in glutathione and antioxidant enzymes, viz., superoxide dismutase (SOD) and catalase levels were observed following MCAO. Pretreatment of Nigella sativa extracts showed the reduction in TBARS, elevation in glutathione, SOD and catalase levels as compared with MCAO rats.Conclusion:The present study observed the neuroprotective effects of both the extracts of Nigella sativa in cerebral ischemia. The neuroprotective effects could be due to its antioxidant, free radical scavenging, and anti-inflammatory properties.

Nigella Sativa Has Beneficial Effect on Osteoporosis and Bone Healing. Is It Fact or Fiction? A Systematic Review

“…She reported that using of oil extract of NS as percutaneous therapy enhances bone healing by enhancing different processes of cell migration and differentiation, extracellular matrix formation, and organization toward calcification. These results are consistent with other same reports [32][33][34][35].…”

A review on therapeutic potential of Nigella sativa: A miracle herb

“…The neuroprotective effects of both the extracts of N. sativa in cerebral ischemia were observed. The neuroprotective effects could be due to its antioxidant, free radical scavenging, and antiinflammatory properties [108] .…”

Neuroprotective Activity of Valeriana officinalis against Global Cerebral Ischemia/Reperfusion Induced Oxidative Stress in Rat

“…In addition, ROS produces malondialdehyde (MDA), an end product of lipid peroxidation. The MDA reacts with TBA and was, thus, estimated as TBARS 21 . Therefore, in the present study, extent of lipid peroxidation in brain homogenate of ischemic rats (control group) was found to be elevated, but in case of EVO treated group LPO levels were significantly reduced, as compared to control group this indicates that ethanol extract of Valeriana officinalis has neuroprotective potential via anti oxidant defence mechanism.…”