Alveolar Cleft Restoration Using Autogenous Tooth Bone Graft Material for Implant Placement: A Case Report

Jeong, Kyung-In; Lee, Junho; Um, In-Woong; Kim, Young-Kyun

doi:10.1563/aaid-joi-d-12-00265

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…[ 3 ] Various in vivo and animal studies have demonstrated its biocompatibility, osteoinductive, and osteoconductive potential. [ 21 22 23 24 25 ]…”

Section: Discussionmentioning

confidence: 99%

Comparative alveolar ridge preservation using allogenous tooth graft versus free-dried bone allograft: A randomized, controlled, prospective, clinical pilot study

et al. 2017

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Background:For the first time in India, allografts from human extracted teeth were prepared. A randomized, prospective, clinicoradiographical, histological study was conducted to evaluate their efficacy in comparison with freeze-dried bone allograft (FDBA) in alveolar ridge preservation.Materials and Methods:Graft preparation: with written consent, teeth were collected from three donors (full mouth extraction cases). Once donors’ serums were tested negative for HIV, HBV, HCV, and Venereal disease research laboratory (VDRL), mineralized whole tooth allograft (WTA) and dentin allograft (DA) were prepared using the standard protocol of Tissue Bank at Tata Memorial Hospital, Mumbai, India.Study Design:In this randomized controlled trial, 15 patients undergoing extraction of at least four teeth were selected. In each patient after atraumatic extractions, one socket was grafted with WTA, second with DA, third with FDBA, and fourth was left ungrafted (control site). All the sites were covered with chorion membrane. To estimate three-dimensional alveolar crest changes, cone beam computed tomography scans were taken immediately after grafting and 4 months postoperatively. Bone biopsies using 3 mm trephine bur were obtained from four patients at the time of implant placement and evaluated histologically.Results:Clinically uneventful healing was observed at all sites. Compared to other sites, WTA and DA consistently showed superior results demonstrating least reduction in alveolar crest height and width which was statistically significant (P < 0.05). Between WTA and DA sites, there was no statistically significant difference. Histological analysis also confirmed more new bone formation at WTA and DA sites.Conclusions:Rather than disposing extracted human teeth as a biomedical waste (common practice), they can be collected from suitable systemically healthy donors. With the help of tissue bank, they can be processed into an allograft, serving as an excellent alternative to conventional allografts.

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“…[ 3 ] Various in vivo and animal studies have demonstrated its biocompatibility, osteoinductive, and osteoconductive potential. [ 21 22 23 24 25 ]…”

Section: Discussionmentioning

confidence: 99%

Comparative alveolar ridge preservation using allogenous tooth graft versus free-dried bone allograft: A randomized, controlled, prospective, clinical pilot study

et al. 2017

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“…Consistent with this study, the present study demonstrated suitable properties for the tooth powder; however, the teeth did not undergo any chemical or thermal processes. The use of autogenous tooth as a bone substitute has been shown in several case-report clinical studies, 15,16 but more research is necessary to find the best and inexpensive processing method to produce a toothderived bone material. 17…”

Section: Discussionmentioning

confidence: 99%

“…The use of autogenous tooth as a bone substitute has been shown in several case-report clinical studies, 15 , 16 but more research is necessary to find the best and inexpensive processing method to produce a tooth-derived bone material. 17 …”

Section: Discussionmentioning

confidence: 99%

Comparison of osteopromoting ability of human tooth powder with the demineralized freeze-dried bone allograft, a bovine xenograft, and a synthetic graft: An in vitro study

Kadkhodazadeh

Fathiazar

Yadegari

et al. 2020

J Adv Periodontol Implant Dent

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Background. The present study aimed to evaluate the osteopromoting ability of human tooth powder and compare it to a bovine xenograft, a synthetic material, and the DFDBA allograft. Methods. In this in vitro study, 30 teeth without caries, inflammation, and infection, which had been extracted for orthodontic reasons, were collected. The crowns were removed, pulpectomy was carried out, and the samples were ground to a powder with particles <500 µm. Osteoblast-like cells of MG-63 were cultured with the tooth powder, Cerabone, DFDBA, and Osteon II. Cell proliferation was assessed by the MTT assay at 24- and 72-hour intervals. The alizarin red test was carried out after three and five days. The alkaline phosphatase level was measured after 24, 48, and 72 hours to assess the osteoblastic activity. The results were analyzed with one-way ANOVA. Results. According to the MTT assay, all the materials exhibited a higher proliferation rate than the control group in 24 hours. In 72 hours, DFDBA had the lowest cell proliferation rate at concentrations of 40 and 80 mg/mL. DFDBA and the positive control group were able to create calcified nodules by the alizarin red test. At the 48- and 72-hour intervals, DFDBA had the lowest alkaline phosphatase activity at a concentration of 40 mg/mL. At the 72-hour interval, bovine xenograft had the highest alkaline phosphatase level, followed by the synthetic material and tooth powder. Conclusion. The tooth powder was able to increase cell proliferation in comparison with the bovine xenograft, the synthetic graft, and the DFDBA. However, its osteopromoting ability was less than that of the osteogenic materials.

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“…Tooth-derived autologous bone graft material has been developed and applied clinically in reconstructive surgery such as alveolar ridge preservation, 5,6 alveolar ridge augmentation, 3,7–9 maxillary sinus augmentation 10 and alveolar cleft reconstruction. 11 The recent systematic review demonstrated that, autologous tooth used for bone grafting showed clinically safe and bone forming capacity with good implant stability. 12…”

Section: Introductionmentioning

confidence: 99%

Chemical and thermal deproteinization of human demineralized tooth matrix: Physicochemical characterization and osteoblast cell biocompatibility

2019

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Although human tooth was developed for using as a bone graft material due to their similarity in chemical composition compared to human bone, the clinical application was limited to autologous use. The aims of this study were to develop the deproteinized human demineralized tooth matrix to be used as a bone graft substitution. The physicochemical characteristic and osteoblastic cell biocompatibility of these materials were analyzed and compared. Human demineralized tooth matrix was prepared by using 0.5 M HCl. Deproteinization of demineralized tooth matrix was performed via three protocols; (a) thermal treatment, (b) NaOH/thermal treatment and (c) H2O2/thermal treatment. Phase and chemical composition were assessed by X-ray diffraction spectroscopy, X-ray fluorescence spectroscopy and Fourier transform infrared analysis. Structural modification was evaluated by scanning electron microscopy. Degradation behavior was evaluated over 60 days. The adhesion and cell proliferation of osteoblast cells were performed on the most appropriate protocol in which proteins were totally eliminated. The results showed that thermal and chemical-thermal deproteinization yielded no distinct morphological and physical changes in deproteinized human demineralized tooth matrix. The scanning electron microscopy examination demonstrated open dentinal tubules with smooth dentin surface in thermal and H2O2/thermal group, while NaOH/thermal group showed rough dentin surface. The X-ray diffraction spectroscopy confirmed that there was only hydroxyapatite phase. The X-ray fluorescence spectroscopy showed that the main components of tooth were calcium (Ca) and phosphorous(P) and the Ca/P molar ratio was about 1.5–1.8. The degradation of the deproteinized human demineralized tooth matrix was slow (Thermal = 9.506 ± 2.568%, NaOH/thermal = 10.244 ± 2.609%, H2O2/thermal = 8.976 ± 2.354%, p < 0.05) at day 60. However, only NaOH/thermal deproteinization demonstrated no protein content. The cells attached and grew on the NaOH/thermal deproteinized human demineralized tooth matrix. The deproteinized human demineralized tooth matrix prepared from the NaOH/thermal protocol demonstrated good physicochemical, and degradation properties that were suitable for preparing bone substitution. In addition, the deproteinized human demineralized tooth matrix scaffold is also favorable for cell attachment and proliferation.

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Alveolar Cleft Restoration Using Autogenous Tooth Bone Graft Material for Implant Placement: A Case Report

Cited by 6 publications

References 13 publications

Comparative alveolar ridge preservation using allogenous tooth graft versus free-dried bone allograft: A randomized, controlled, prospective, clinical pilot study

Comparative alveolar ridge preservation using allogenous tooth graft versus free-dried bone allograft: A randomized, controlled, prospective, clinical pilot study

Comparison of osteopromoting ability of human tooth powder with the demineralized freeze-dried bone allograft, a bovine xenograft, and a synthetic graft: An in vitro study

Chemical and thermal deproteinization of human demineralized tooth matrix: Physicochemical characterization and osteoblast cell biocompatibility

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