Background. The present study aimed to evaluate the osteopromoting ability of human tooth powder and compare it to a bovine xenograft, a synthetic material, and the DFDBA allograft. Methods. In this in vitro study, 30 teeth without caries, inflammation, and infection, which had been extracted for orthodontic reasons, were collected. The crowns were removed, pulpectomy was carried out, and the samples were ground to a powder with particles <500 µm. Osteoblast-like cells of MG-63 were cultured with the tooth powder, Cerabone, DFDBA, and Osteon II. Cell proliferation was assessed by the MTT assay at 24- and 72-hour intervals. The alizarin red test was carried out after three and five days. The alkaline phosphatase level was measured after 24, 48, and 72 hours to assess the osteoblastic activity. The results were analyzed with one-way ANOVA. Results. According to the MTT assay, all the materials exhibited a higher proliferation rate than the control group in 24 hours. In 72 hours, DFDBA had the lowest cell proliferation rate at concentrations of 40 and 80 mg/mL. DFDBA and the positive control group were able to create calcified nodules by the alizarin red test. At the 48- and 72-hour intervals, DFDBA had the lowest alkaline phosphatase activity at a concentration of 40 mg/mL. At the 72-hour interval, bovine xenograft had the highest alkaline phosphatase level, followed by the synthetic material and tooth powder. Conclusion. The tooth powder was able to increase cell proliferation in comparison with the bovine xenograft, the synthetic graft, and the DFDBA. However, its osteopromoting ability was less than that of the osteogenic materials.
Background and AIM: Plaque-induced gingivitis is a common gingival disease. There are some limitations in mechanical methods to promote plaque control which is why chemical methods have been introduced. Chemical toothpastes have side effects like hypersensitivity and staining, so the tendency is growing towards herbal toothpastes. Our aim in this study was to evaluate the effectiveness of Aloevera tooth paste on gingivitis. Materials and methods: This was a Single blind cross over clinical trial study which was conducted on 20 dental students (10 male, 10 female with the average of 24.5±4 years) who had gingivitis. Then, they were divided into 2 groups: The first group used the Aloevera toothpaste for 30 days and PI and GI was recorded. They had been washed out for 2 weeks and after that they used the Fluoride toothpaste for another 30 days. Again, PI and GI was recorded. In the second group the toothpastes were used in an opposite order. The Wilcoxon test was used to evaluate statistical analysis.the level of significance was set as 0.06. Results:There was no significant difference in periodontal records between toothpastes after 30 days of administration. After 30 days, PI changed from 2.14±1.3 to 1.84±1.02(P<0.098) and GI changed from 0.74 ±0.62 to 0.25±0.46(P<0.068) and the P-value between the toothpastes was P<0.07 for PI and P<0.308 for GI. Conclusion: Aloevera toothpaste may have the same effect on PI and GI as the Fluoride tooth paste, So it can be concluded the this toothpaste could be used instead of conventional chemical toothpastes.
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