Alveolar bubble-stability method in the study of lung alveolar surfactant.

Slavkovic, V.S.; Wingo, L E; Ellison, Robert G.; Ellison, Lois T.

doi:10.1152/jappl.1968.24.4.510

Cited by 13 publications

(3 citation statements)

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“…There are different opinions on the reliability of SRs values of samples, which were not examined fresh. Slavkovic et al [1968] indicated slightly decreased values 6 h after death of a dog; a significant decrease in values was demonstrated after I week of freezer storage and after 2 months again. Pattle [1962] found no important changes in SR after 3 weeks" refrigeration and G r ü n e w a l d el al.…”

Section: Methodsmentioning

confidence: 90%

“…The shorter the time inter val studied, the higher was the number of bubbles of lower SR. As the time of the 1st bubble measurement is usually not indicated in the literature, a variability of this factor might contribute to a difference of results among various laboratories. Pattle [1958] recommended to select all suitable bubbles within 3 min; Slavkovic et al [1968] followed Pattle's suggestion regarding time and used the interval of 2 min. This interval has been quite satisfactory in our study; it has enabled us to choose a microscopic field with sufficient accuracy.…”

Section: Methodsmentioning

confidence: 99%

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Pattle’s Bubble-Stability Method in the Rat

Havránková¹,

Kunc²,

Kuncová³

1975

Respiration

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The following report deals with Pattle’s alveolar bubble-stability method for surfactant studies in rats. There was no difference in stability ratio of bubbles obtained from separate lobes without gross pathological lesions. Bubble stability was not influenced by microscopic illumination (used only briefly for photographing) and by 1-day storage at +4°C. The stability ratio was significantly lower in distilled water in comparison with the value obtained in isotonic saline. If the time interval passing from squeezing of the bubbles to the time of the first measurement is strictly retained, the reliability of the method is satisfactory. The use of cumulative distribution curves of individual stability ratios’ values and non-parametric testing of such results is recommended.

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Section: Methodsmentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%