Alveolar bone osteoblast differentiation and Runx2/Cbfa1 expression

Perinpanayagam, Hiran; Martın, Thomas; Mithal, Vartika; Dahman, M.; Marzec, N.; Lampasso, Judith; Dziak, Rosemary

doi:10.1016/j.archoralbio.2005.09.004

Cited by 30 publications

(22 citation statements)

References 46 publications

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“…Osteoblast differentiation is a finely regulated process in which the expression of several genes is highly controlled [37][38][39]. In this scenario, the transcription factor RUNX2 plays an essential role since it binds and regulates the expression of multiple genes expressed in osteoblasts [40].…”

Section: Discussionmentioning

confidence: 99%

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

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Bacterial infection in dental implants along with osseointegration failure usually leads to loss of the device. Bioactive molecules with antibacterial properties can be attached to titanium surfaces with anchoring molecules such as silanes, preventing biofilm formation and improving osseointegration. Properties of silanes as molecular binders have been thoroughly studied, but research on the biological effects of these coatings is scarce. The aim of the present study was to determine the in vitro cell response and antibacterial effects of triethoxysilypropyl succinic anhydride (TESPSA) silane anchored on titanium surfaces. X-ray photoelectron spectroscopy confirmed a successful silanization. The silanized surfaces showed no cytotoxic effects. Gene expression analyses of Sarcoma Osteogenic (SaOS-2) osteoblast-like cells cultured on TESPSA silanized surfaces reported a remarkable increase of biochemical markers related to induction of osteoblastic cell differentiation. A manifest decrease of bacterial adhesion and biofilm formation at early stages was observed on treated substrates, while favoring cell adhesion and spreading in bacteria-cell co-cultures.Surfaces treated with TESPSA could enhance a biological sealing on implant surfaces against bacteria colonization of underlying tissues. Furthermore, it can be an effective anchoring platform of biomolecules on titanium surfaces with improved osteoblastic differentiation and antibacterial properties.

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Section: Discussionmentioning

confidence: 99%

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

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“…Cell attachment is one of the most sensitive methods for measuring toxicity. Different cells, such as MG-63 osteosarcoma cells (Koh et al 1998), Saos-2 osteosarcoma cells (Zhu et al 2000), human gingival fibroblasts, periodontal ligament fibroblasts (Bonson et al 2004), human alveolar bone cells (Perinpanayagam et al 2006), and cementoblast cell lines (Thomson et al 2003), have been used for the evaluation of MTA biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

Morphological behavior and attachment of p19 neural cells to root‐end filling materials

Saghiri¹,

Asgar²,

Daliri³

et al. 2010

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Some techniques and instruments like stereomicroscopy and confocal microscopy used for observing neural cells are too complicated and dependent on preparation and cell fixation methods. This may question the results of these methods. Though, we have used scanning electron microscopy on replicated specimens to observe p19 neural cells and their cellular extensions. This manuscript has shown the feasibility of using replica (indirect) method instead of direct methods for observing morphological characteristics of this high sensitive cell line. As neural cells are very sensitive to fixation solutions and processes, we have used replica mode and observed neural cells with a novel indirect method. We have used replica mode in this study to indirectly and noninvasively evaluate the state of p19 neural cells and their cellular extensions.

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“…RUNX2 expression can also be detected in the osteoblasts of alveolar bone of adult humans ( Figure 1). If these are osteogenically stimulated, expression is increased [65]. RUNX2 can also be demonstrated in newly-formed fibrous bone around radicular cysts or peri-apical processes [48,64].…”

Section: Funktionen Von Runx2mentioning

confidence: 99%

“…Eine RUNX2-Expression lässt sich auch in Osteoblasten des Alveolarknochens erwachsener Menschen nachweisen (Abbildung 1). Werden diese osteogen stimuliert, kommt es zu einer verstärkten Expression [65]. Auch im neu gebildeten fibrösen Knochen um radikuläre Zysten oder periapikale Prozesse lässt sich RUNX2 nachweisen [48,64].…”

Section: Funktionen Von Runx2unclassified

Die Bedeutung von RUNX2 in der postnatalen Entwicklung des Kondylus

et al. 2010

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We observed that RUNX2 is involved in normal condylar growth in the mouse and probably plays a significant role in osteogenesis and angiogenesis. The RUNX2 also has a biomechanical correlation in relation to cartilage compartmentalization. At the protein level, we noted no differences in the occurrence and distribution of RUNX2 in the condyle, except for a short phase during the 4th and 6th postnatal weeks, so that one allele might suffice for largely normal growth; other biological factors may have compensatory effects. However, we did observe small changes in a few cephalometric parameters concerning the mandibles of heterozygous knockout animals. We discuss potential correlations to our findings by relating them to the most current knowledge about the RUNX2 biology.

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Alveolar bone osteoblast differentiation and Runx2/Cbfa1 expression

Cited by 30 publications

References 46 publications

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Morphological behavior and attachment of p19 neural cells to root‐end filling materials

Die Bedeutung von RUNX2 in der postnatalen Entwicklung des Kondylus

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