Morphological behavior and attachment of p19 neural cells to root‐end filling materials

Abstract

Some techniques and instruments like stereomicroscopy and confocal microscopy used for observing neural cells are too complicated and dependent on preparation and cell fixation methods. This may question the results of these methods. Though, we have used scanning electron microscopy on replicated specimens to observe p19 neural cells and their cellular extensions. This manuscript has shown the feasibility of using replica (indirect) method instead of direct methods for observing morphological characteristics of this high sensitive cell line. As neural cells are very sensitive to fixation solutions and processes, we have used replica mode and observed neural cells with a novel indirect method. We have used replica mode in this study to indirectly and noninvasively evaluate the state of p19 neural cells and their cellular extensions.

Effect of biomaterials on angiogenesis during vital pulp therapy

“…It is defined as the surgical removal of inflamed coronal part of the dental pulp in the exposed pulpal tissue to save the remaining healthy tissue 5) . In dentin-pulp complex regeneration, clinicians attempt to provide an effective pulp capping with appropriate sealing ability 5) , and maintain the vitality of irritated pulp tissues and promote the formation of a dentinal bridge 8–10) and other tissues including neural cells 11) . In these procedures, the progenitor dental pulp stem cells (DPSCs) are migrated, recruited, and differentiated into odontoblast-like cells, which have the ability to produce reparative dentin 9,12) .…”

Mechanical Response of Dental Cements as Determined by Nanoindentation and Scanning Electron Microscopy

“…There is almost no doubt that calcium silicate cements such as mineral trioxide aggregate (MTA) as a root-end filling and repair material are biocompatible (Saghiri et al, 2010 a ), have dentinogenic properties (Economides et al, 2003), and have superior sealing ability in comparison with other cements (Torabinejad et al, 1993; Bates et al, 1996). Microleakage prevention and antibacterial efficiency of this cement against microorganisms and their by-products can play an important role in reducing the failure of endodontic treatments (Torabinejad et al, 1995 a , 1995 b ; Bates et al, 1996).…”

Effect of particle size on calcium release and elevation of pH of endodontic cements

“…One such material, introduced in 1993, was mineral trioxide aggregate (MTA) . MTA soon became a reliable alternative for calcium hydroxide due to its remarkable and superior characteristics . MTA cement has an insoluble matrix made of silica, which gives MTA better stability than calcium hydroxide, and prevents it from being washed out from applied areas .…”