Altered osmotic swelling behavior of proteoglycan-depleted bovine articular cartilage using high frequency ultrasound

Wang, Q.; Zheng, Yongping; Leung, Gina; Lam, W.L.; Guo, Xia; Lu, Hongbin; Qin, Ling; Mak, Arthur F.T.

doi:10.1088/0031-9155/53/10/006

Cited by 21 publications

(17 citation statements)

References 43 publications

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“…Furthermore, rats in the TS group stayed in a head-down position, which induced changes in fluid distribution. Because 60-80% of ECM is interstitial water and articular cartilage contacts with synovial fluid, the fluid shifts affect PG synthesis (Buschmann et al 1999) and induce the swelling behavior of articular cartilage (Mow et al 2005;Wang and Zheng 2006;Wang et al 2008b). However, the thickness of articular cartilage at different anatomical locations did not alter at the same extent.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound Evaluation of Site-Specific Effect of Simulated Microgravity on Articular Cartilage

Wang

Zheng

Wang

et al. 2010

Ultrasound in Medicine & Biology

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Section: Discussionmentioning

confidence: 99%

Ultrasound Evaluation of Site-Specific Effect of Simulated Microgravity on Articular Cartilage

Wang

Zheng

Wang

et al. 2010

Ultrasound in Medicine & Biology

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“…Already the determination of cartilage thickness as well as observing its osmotic swelling behavior is performed with a multitude of techniques. Methods described in the literature include a digital micrometer, high frequency ultrasound, 8,9 a laser-based system, 10 a confined chamber, 11 and a needle probe measurement. 12 All of these techniques come with certain advantages and disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Adapting a commercial shear rheometer for applications in cartilage research

Boettcher

Grumbein

Winkler

et al. 2014

Review of Scientific Instruments

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Cartilage research typically requires a broad range of experimental characterization techniques and thus various testing setups. Here, we describe how several of those tests can be performed with a single experimental platform, i.e. a commercial shear rheometer. Although primarily designed for shear experiments, such a rheometer can be equipped with different adapters to perform indentation and creep measurements, quantify alterations in the sample thickness, and conduct friction measurements in addition to shear rheology. Beyond combining four distinct experimental methods into one setup, the modified rheometer allows for performing material characterizations over a broad range of time scales, frequencies, and normal loads.

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“…Cartilage-bone plugs with a diameter of approximately 6-6.35 mm were commonly adopted in the studies on cartilage swelling using various methods such as compression [6], water weight gain [7,8], and ultrasound scanning [9][10][11][12][13]. Among those methods, ultrasound has advantages such as noninvasiveness and easy access with acceptably low cost.…”

Section: Introductionmentioning

confidence: 99%

“…Secondly, different from the osmotic-mechanical combined loading, only an osmotic loading by varying the concentration of the bathing saline solution without external mechanical loading has been applied to explore the free swelling of articular cartilage [5,9,10]. Ultrasound has been used to monitor the free swelling in a noncontact way [9][10][11][12][13]. In those studies, the scanning site was always chosen at the central area of the sample to avoid the effect of the cut edges, which greatly depends on the integrity of the sample itself.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound Assessment of Boundary Effect on Osmosis-Induced Shrinkage and Swelling of Articular CartilageIn Vitro

Wang

Zheng

2012

Connective Tissue Research

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This study aims to use ultrasound to investigate the boundary effect of the cut edge on the osmosis-induced shrinkage and swelling of articular cartilage while the distance from the scanning site to the edge decreases with the reduction of specimen size. Sixteen cartilage-bone specimens (of diameter 6.35 mm) were prepared from normal bovine patellae. The cartilage width was gradually reduced to 4.35 mm and to 2.35 mm. Shrinkage and swelling were induced by changing the concentration of the saline solution and monitored using nominal 50 MHz focused ultrasound. The parameters including shrinkage and swelling peak strains (ϵ(P1) and ϵ(P2), respectively), shrinkage and swelling equilibrium strains (ϵ(E1) and ϵ(E2), respectively), and shrinkage and swelling slopes (k(1) and k(2), respectively) were extracted. The ϵ(P1), ϵ(E1), ϵ(E2), k(1), and k(2) of the 2.35 mm specimens were significantly different (p < 0.05) from those of the 6.35 mm specimens. For the 4.35 mm specimens, ϵ(E1) and ϵ(P1) were, respectively, significantly different (p < 0.05) from ϵ(E1) of the 6.35 mm specimens and ϵ(P1) of the 2.35 mm specimens. The percentage of coefficient of variation (18.5% for shrinkage and 16.3% for swelling) of the 2.35 mm specimens was much higher than that (<8.5%) of the 6.35 mm specimens. The relative root mean square difference (rRMSD%, 12.0% for shrinkage and 10.6% for swelling) of the 2.35 mm specimens was also much higher than that (<5.5%) of the 6.35 mm specimens. The results indicated that the boundary effect of the cut edge on the osmosis-induced shrinkage and swelling of articular cartilage increases with the reduction of specimen size.

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Altered osmotic swelling behavior of proteoglycan-depleted bovine articular cartilage using high frequency ultrasound

Cited by 21 publications

References 43 publications

Ultrasound Evaluation of Site-Specific Effect of Simulated Microgravity on Articular Cartilage

Ultrasound Evaluation of Site-Specific Effect of Simulated Microgravity on Articular Cartilage

Adapting a commercial shear rheometer for applications in cartilage research

Ultrasound Assessment of Boundary Effect on Osmosis-Induced Shrinkage and Swelling of Articular CartilageIn Vitro

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