Adapting a commercial shear rheometer for applications in cartilage research

Boettcher, Kathrin; Grumbein, Stefan; Winkler, U; Nachtsheim, Julia; Lieleg, Oliver

doi:10.1063/1.4894820

Cited by 31 publications

(21 citation statements)

References 22 publications

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“…Furthermore, the gelation profile showed that the hydrogel system provided a suitable time window between the gelation point at about 2 min, after which embedded cells do not rapidly sink to the bottom any more, and an ongoing gelation and crosslinking for over 1 h, to result in well‐distributed cells incorporated within the hydrogels. The hydrogels were observed to be considerably softer in comparison to native cartilage, for which an elastic modulus of ≈0.4–0.6 MPa was reported . However, a hydrogel stiffness similar to that determined for the hydrogels developed in this study has previously been shown to be suitable in cartilage engineering for robust chondrogenic differentiation of MSCs …”

Section: Resultssupporting

confidence: 68%

TGF‐β1‐Modified Hyaluronic Acid/Poly(glycidol) Hydrogels for Chondrogenic Differentiation of Human Mesenchymal Stromal Cells

Böck

Schill

Krähnke

et al. 2018

Macromolecular Bioscience

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In cartilage regeneration, the biomimetic functionalization of hydrogels with growth factors is a promising approach to improve the in vivo performance and furthermore the clinical potential of these materials. In order to achieve this without compromising network properties, multifunctional linear poly(glycidol) acrylate (PG-Acr) is synthesized and utilized as crosslinker for hydrogel formation with thiol-functionalized hyaluronic acid via Michael-type addition. As proof-of-principle for a bioactivation, transforming growth factor-beta 1 (TGF-β1) is covalently bound to PG-Acr via Traut's reagent which does not compromise the hydrogel gelation and swelling behavior. Human mesenchymal stromal cells (MSCs) embedded within these bioactive hydrogels show a distinct dose-dependent chondrogenesis. Covalent incorporation of TGF-β1 significantly enhances the chondrogenic differentiation of MSCs compared to hydrogels with supplemented noncovalently bound TGF-β1. The observed chondrogenic response is similar to standard cell culture with TGF-β1 addition with each medium change. In general, multifunctional PG-Acr offers the opportunity to introduce a range of biomimetic modifications (peptides, growth factors) into hydrogels and, thus, appears as an attractive potential material for various applications in regenerative medicine.

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Section: Resultssupporting

confidence: 68%

TGF‐β1‐Modified Hyaluronic Acid/Poly(glycidol) Hydrogels for Chondrogenic Differentiation of Human Mesenchymal Stromal Cells

Böck

Schill

Krähnke

et al. 2018

Macromolecular Bioscience

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“…[ 53 ] and shown as an inset in Figure 2 B. As friction partners in the tribology setup, PDMS cylinders and steel spheres with a diameter of 12.7 mm (Kugel Pompel) were chosen.…”

Section: −3mentioning

confidence: 99%

Modulating Mucin Hydration and Lubrication by Deglycosylation and Polyethylene Glycol Binding

Crouzier

Boettcher

Geonnotti

et al. 2015

Adv Materials Inter

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glycoproteins, a family of high molecular weight and densely glycosylated biopolymers. Those mucins can act as an infection shield by trapping various viruses in the biopolymer matrix [ 1 ] and they reduce bacterial adhesion to surfaces. [ 2,3 ] Mucins also form highly hydrated and lubricative layers that protect the underlying epithelium from dehydration and shear stress that emerge during eye blinking or when swallowing food. Water typically accounts for up to 95% of the total mass in the mucus gel. [ 4,5 ] Shifts in the mucus water content correlate with substantial changes of the mucus barrier function [6][7][8] and result in important pathological disorders such as dry mouth [ 9 ] and dry eye. [ 10 ] Mucin-associated glycans contribute up to 80% of the molecular weight of mucins [ 11 ] and contain highly hydrated hydroxyl groups. Differences in the amount and composition of glycans from mucins extracted from pig stomachs and mucins from bovine submaxillary glands correlate with their capacity to adsorb water. [ 12 ] However, other properties such as protein composition also differ between these two mucin species, precluding the establishment of a defi nitive link between the presence of mucin glycans and hydration.Mucins, and other well-hydrated molecules such as zwitterionic polymers, polysaccharides, and polyelectrolytes are generally good boundary lubricants when adsorbed [ 13,14 ] or assembled in brush structures [15][16][17][18] on surfaces. Hence, mucin-associated glycans could also be essential for the lubricative function of mucus. In a related system, the mucin-like macromolecule lubricin showed a signifi cant decrease in lubricity when its associated glycans were removed. [ 19 ] Moreover, the lubricity of salivary fi lms, which contain mucins, has also been correlated with the total amount of glycosylation present in the fi lm. However, also here, the complexity of saliva composition precluded the identifi cation of the specifi c role of mucin glycosylation. [ 20 ] Hence, there is a need for a more defi ned experimental system to determine if mucin glycosylation is essential to the hydration and lubrication properties of mucins.This study explores the role of glycosylation in mucin hydration and lubricity, using mucin coatings as a simplifi ed model system. Such mucin coatings can reconstitute levels of surface hydration [ 21,22 ] and lubrication [23][24][25] similar to those found in native mucus. Our data show that the removal of mucin-bound glycans results in a signifi cant reduction in hydration and lubricity of the mucin-coated surface. We show that the hydration and lubricity of deglycosylated mucins can be partially A key property of mucin glycoproteins is their exceptional capacity to hydrate and lubricate surfaces. In vivo, mucins assemble into mucus hydrogels that cover the epithelium and protect it from dehydration and shear stress. A better understanding of the origin of these properties could lead to new treatment strategies for patients with poor mucus coverage, defective mucus produ...

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“…We next repeated our comparison of different lubricants with a macroscopic method, i.e. a rotational macrotribometer (see Boettcher et al, 2014 for more details). Here, a glass sphere in contact with three cartilage samples was rotated with a constant velocity (Fig.…”

Section: Macro-friction Experimentsmentioning

confidence: 99%

“…The test procedure was described in detail by Boettcher et al (2014). In short, a glass sphere with a diameter of ½ in.…”

Section: Rotational Tribometermentioning

confidence: 99%

“…We chose two complementary methods: atomic force microscopy (AFM) and a rotational tribology setup (Boettcher et al, 2014). Owing to the small contact area, the AFM allows to probe boundary lubrication (Park et al, 2004) whereas with the static contact of the tribometer the effect of the interstitial fluid pressurization can be investigated (Caligaris and Ateshian, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of friction and wear of articular cartilage on different length scales

Kienle

Boettcher

Wiegleb

et al. 2015

Journal of Biomechanics

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Adapting a commercial shear rheometer for applications in cartilage research

Cited by 31 publications

References 22 publications

TGF‐β1‐Modified Hyaluronic Acid/Poly(glycidol) Hydrogels for Chondrogenic Differentiation of Human Mesenchymal Stromal Cells

TGF‐β1‐Modified Hyaluronic Acid/Poly(glycidol) Hydrogels for Chondrogenic Differentiation of Human Mesenchymal Stromal Cells

Modulating Mucin Hydration and Lubrication by Deglycosylation and Polyethylene Glycol Binding

Comparison of friction and wear of articular cartilage on different length scales

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