Alterations in the Growth and Adhesion Pattern of Vero Cells Induced by Nutritional Stress Conditions

Abstract

The pattern of growth, adhesion and protein synthesis in Vero cells submitted to nutritional stress conditions was investigated. The control cells presented a characteristic pattern, with monolayer growth, while the stressed cells presented multilayered growth, with aggregate or spheroid formation which detached on the flask surface and continued their growth in another region. In the soft agar assay, with reduced amount of nutrients, only the stressed cells presented growth, indicating physical and nutritional independence. A 44-kDa protein was observed in stressed cells and was absent in non-stressed cells. The adhesion index and fibronectin synthesis and distribution were altered in stressed cells. After confluence, control cells presented fibronectin accumulation in lateral cell-cell contact regions, while this fibronectin accumulation pattern was not observed in stressed cells. These alterations may be responsible for the multilayered growth and decreased adhesion index observed in stressed cells which were transformed by nutritional stress conditions.

Volume 12, Issue 3

“…In summary, the results presented here confirm the essential roles that media change and sub-culturing procedures play in maintaining the morphological integrity of Vero cells, as shown previously by Genari et al (1998). 10 These results also demonstrate the utility of Vero cells as a model to study cell transformation induced by artificially implemented nutritional stress paradigms in vitro.…”

Cytotoxicity of tamoxifen in normal and tumoral cell lines and its ability to induce cellular transformation in vitro

“…This may eventually trigger uncontrolled cell growth and the formation of multiple-layers (Genari et al, 1996(Genari et al, , 1998. Such changes were observed in scanning electron micrographs of V79 cells exposed to TAM, DMBA and DMBA C TAM.…”

“…Studies with fibroblasts transformed in vitro have shown a decrease in fibronectin on the cell surface (Santi and Zardi 1985), that can adversely effect cellecell and cellesubstrate interactions and lead to a loss of growth by contact inhibition and anchorage-dependency. This may eventually trigger uncontrolled cell growth and the formation of multiple-layers (Genari et al, 1996(Genari et al, , 1998. Such changes were observed in scanning electron micrographs of V79 cells exposed to TAM, DMBA and DMBA C TAM.…”

Cell Adhesion to Plasma-Coated PVC

“…The cells are elongated and present cytoplasmatic projections. Vero cells which normally grow in monolayers are elongated and present particulates, being similar to fibroblastic cells [32]. …”