Alterations in Specific Gravity during the Ripening of Bull Spermatozoa

Lindahl, Per; Kihlström, J. E.

doi:10.3168/jds.s0022-0302(52)93721-1

Cited by 50 publications

(33 citation statements)

References 6 publications

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“…The determined density of 1.233 g/ml is similar to the values obtained by Lindahl and Kihlström [1] and Lindahl and Thungvist [4]). As the gradients used by these workers killed the sperm these values were presumably for killed sperm.…”

Section: Discussionsupporting

confidence: 76%

“…Under the conditions studied the density of bull sperm was reported by Lindahl and Kihlström [1] and Lavon et al [5] reported that bull sperm increase in density as they mature. Benedict et al [15], using older samples of bull sperm, reported buoyant density of a low density class of sperm to be 1.16 to 1.19 g/ml and of a high density class to be 1.25 to 1.32 g/ml.…”

Section: Discussionmentioning

confidence: 72%

“…Oshio [3] reported a mean value of bull sperm density of 1.12 g/ml with a considerable range in densities associated with sperm motility. The density of sperm changes during maturation in the epididymis [1,4,5]. So differences in estimated density of sperm can be accounted for partly by the age and maturity of sperm as well as the techniques used to estimate sperm density.…”

mentioning

confidence: 99%

“…Lindahl and Kihlström [1] reported that the density of bull sperm varied between 1.241 and 1.334 g/ml. Lavon et al [2] reported a bull sperm density range from 1.0376 to 1.0927 g/ml.…”

mentioning

confidence: 99%

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Effect of Density Gradient Osmolality on Specific Gravity of Bull Sperm and Their Separation, but Not on the Basis of Sex.

Hough

Foote

2002

Journal of Reproduction and Development

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Abstract.The most effective quantitative separation of ejaculates of semen into subsets of sperm differing in quality by density gradients requires information on the specific effects of processing conditions on sperm density. Reported density of bull sperm varies greatly. This research was undertaken to study the effects of gradient osmolality on sperm density and on separation using gradients with low viscosity and low toxicity to sperm. Semen was available from bulls used for artificial insemination. Cell volume studies revealed that live sperm volume was altered by more than 25% by exposure to solutions differing in osmolality, resulting in predictable differences in density measurable by density gradients. Control studies indicated that discontinuous Nycodenz ® gradients differing by less than 1% in density could be prepared. Following centrifugation at 100,000 × g for 1 h fractions containing 96-99% unstained (live) and 91% motile sperm versus a fraction with 100% nonmotile sperm were obtained. The densities (g/ml) of live sperm separated on low osmolality gradients (220-237 mOsm), and on high osmolality gradients (342-345 mOsm) were 1.125 versus 1.145, respectively. Killed sperm on both gradients had a density of 1.233 g/ml. These gradients permit quantitative separation of sperm varying in membrane integrity and ability to function under different conditions, but do not permit sexing.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 72%

mentioning

confidence: 99%

“…Lindahl and Kihlström [1] reported that the density of bull sperm varied between 1.241 and 1.334 g/ml. Lavon et al [2] reported a bull sperm density range from 1.0376 to 1.0927 g/ml.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Effect of Density Gradient Osmolality on Specific Gravity of Bull Sperm and Their Separation, but Not on the Basis of Sex.

Hough

Foote

2002

Journal of Reproduction and Development

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show abstract

“…The physiological maturation of mammalian spermatozoa implies inter alia an elimination of water from the cell constituents (Lindahl & Kihlström, 1952a). It is to be expected that this process involves also the outermost layers of the cells, i.e.…”

Section: Methodsmentioning

confidence: 99%