Effect of Density Gradient Osmolality on Specific Gravity of Bull Sperm and Their Separation, but Not on the Basis of Sex.

Hough, Shelley R.; Foote, R.H.

doi:10.1262/jrd.48.399

Cited by 4 publications

(1 citation statement)

References 23 publications

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“…Lindahl and Kihlström [ 97 ] reported normal bull sperm density varying between 1.241 and 1.334 g/mL. On the other hand, Lavon et al [ 98 ] reported a range from 1.0376 to 1.0927 g/mL, because the density of sperm may change with age [ 99 ]. The basis of this separation type is a colloidal medium formed by silica particles coated with covalently bonded hydrophilic silane in HEPES [ 100 ].…”

Section: Centrifugation Techniquesmentioning

confidence: 99%

Strategies for Bacterial Eradication from Human and Animal Semen Samples: Current Options and Future Alternatives

Ďuračka

Benko

Chňapek

et al. 2023

Sensors

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The primary role of semen processing and preservation is to maintain a high proportion of structurally and functionally competent and mature spermatozoa, that may be used for the purposes of artificial reproduction when needed, whilst minimizing any potential causes of sperm deterioration during ex vivo semen handling. Out of a multitude of variables determining the success of sperm preservation, bacterial contamination has been acknowledged with an increased interest because of its often unpredictable and complex effects on semen quality. Whilst antibiotics are usually the most straight-forward option to prevent the bacterial contamination of semen, antimicrobial resistance has become a serious threat requiring widespread attention. As such, besides discussing the consequences of bacteriospermia on the sperm vitality and the risks of antibiotic overuse in andrology, this paper summarizes the currently available evidence on alternative strategies to prevent bacterial contamination of semen prior to, during, and following sperm processing, selection, and preservation. Alternative antibacterial supplements are reviewed, and emphasis is given to modern methods of sperm selection that may be combined by the physical removal of bacteria prior to sperm preservation or by use in assisted reproductive technologies.

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